Engineering of biological pacemaker with autonomic innervation

• Project/Area Number: 20590861
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Circulatory organs internal medicine
• Research Institution: Nagoya University
• Principal Investigator: LEE Jong-Kook Nagoya University, 医学系研究科, 寄附講座准教授 (60303608)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 心筋再生 / バイオペースメーカ / 自律神経 / 不整脈 / 細胞移植 / 交感神経

Research Abstract

The present study showed that neurotrophic factors play important roles in axon guidance of sympathetic neurons to cardiomyocytes. The results suggest that appropriate use of neurotrophic factors enable autonomic innervations in transplanted cardiomyocytes. Thus, the technique may be an important step to engineer functional myocardium in myocardial regeneration therapy.

Research Products

• [Journal Article] Glial cell line-derived neurotrophic factor (GDNF) enhances sympathetic neurite growth in rat hearts at early developmental stages. (2010)
  • Author(s): Miwa K, Lee JK, Takagishi Y, Opthof T, Fu X, Kodama I.
  • Journal Title: Biomed Res 31(6) Pages: 353-61
• [Journal Article] The cellular prion protein identifies bipotential cardiomyogenic progenitors. (2010)
  • Author(s): Hidaka K, Shirai M, Lee JK, Wakayama T, Kodama I, Schneider MD, Morisaki T.
  • Journal Title: Circ Res 106(1) Pages: 111-9
• [Journal Article] Glial cell line-derived neurotrophic factor (GDNF) enhances sympathetic neurite growth in rat hearts at early developmental stages. (2010)
  • Author(s): Miwa, et al.
  • Journal Title: Biomed Res Volume: 31(6) Pages: 353-361
  • Peer Reviewed
• [Journal Article] The cellular prion protein identifies bipotential cardiomyogenic progenitors. (2010)
  • Author(s): Hidaka, et al
  • Journal Title: Circ Res Volume: 106 Pages: 111-119
  • Peer Reviewed
• [Journal Article] Effects of aldosterone on Cx43 gap junction expression in neonatal rat cultured cardiomyocytes. (2009)
  • Author(s): Suzuki S, Ohkusa T, Sato T, Yoshida M, Yasui K, Miwa K, Lee JK, Yano M, Kodama I, Matsuzaki M.
  • Journal Title: Circ J 73 Pages: 1504-1512
• [Journal Article] Effects of aldosterone on Cx43 gap junction expression in neonatal rat cultured cardiomyocytes. (2009)
  • Author(s): Suzuki, et al
  • Journal Title: Circ J Volume: 73 Pages: 1504-1512
  • Peer Reviewed
• [Journal Article] Paracrine factors of vascular endothelial cells facilitate cardiomyocyte differentiation of mouse embryonic stem cells. (2008)
  • Author(s): Kado M, Lee JK, Hidaka K, Miwa K, Murohara T, Kasai K, Saga S, Morisaki T, Ueda Y, Kodama I.
  • Journal Title: Biochem Biophys Res Commun 377:2 Pages: 413-418
• [Journal Article] Paracrine factors of vascular endothelial cells facilitate cardiomyocyte differentiation of mouse embryonic stem cells. (2008)
  • Author(s): Kado M, et al
  • Journal Title: Biochemical and Biophysical Research Communications 377 Pages: 413-418
  • Peer Reviewed
• [Presentation] 再生心筋への交感神経支配の確立 (2010)
  • Author(s): Lee JK, et. al.
  • Organizer: 日本心不全学会学術集会シンポジウム
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2010-10-08
• [Presentation] Axon guidance to induced pluripotent stem cell-derived cardiac cells by glial cell line-derived neurotrophic factor (GDNF). (2010)
  • Author(s): Miwa K, Lee JK, et. al.
  • Organizer: The 74th Annual Scientific Meeting of the Japanese Circulation Society.
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-05
• [Presentation] Axon guidance to induced pluripotent stem cell-derived cardiac cells by glial cell line-derived neurotrophic factor (GDNF) (2010)
  • Author(s): Miwa K, Lee, et al
  • Organizer: The 74^<th> Annual Scientific Meeting of the Japanese Circulation Society
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-03-05
• [Presentation] Axon guidance of sympathetic neurons to native and induced pluripotent stem (iPS) cell-derived cardiomyocytes using glial cell line-derived neurotrophic factor (GDNF). (2009)
  • Author(s): Miwa K, Lee JK, et. al.
  • Organizer: Scientific Sessions 2009 America Heart Association.
  • Place of Presentation: Orlando, USA
  • Year and Date: 2009-11-17
• [Presentation] Axon guidance of sympathetic neurons to native and induced pluripotent stem (iPS) cell-derived cardiomyocytes using glial cell line-derived neurotrophic factor (GDNF) (2009)
  • Author(s): Miwa K, Lee, et al
  • Organizer: Scientific Sessions 2009 America Heart Association
  • Place of Presentation: Orlando, USA
  • Year and Date: 2009-11-17
• [Presentation] Axonal guidance for sympathetic neuron to cardiac myocyte by glial cell line-derived neurotrophic factor. (2008)
  • Author(s): Miwa K, Lee JK, et. al.
  • Organizer: The 25th Annual meeting of international society for heart research Japanese section.
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-05
• [Presentation] Axonal guidance for sympathetic neuron to cardiac myocyte by glial cell line-derived neurotrophic factor (2008)
  • Author(s): Miwa K, Lee, et al
  • Organizer: The 25^<th> Annual meeting of international society for heart research Japanese section
  • Place of Presentation: 横浜
  • Year and Date: 2008-12-05
• [Presentation] A novel technology to prevent proarrhythmia of myoblast transplantation : "Patterning" of cell alignment using magnetic nanoparticles. (2008)
  • Author(s): Takanari H, Lee JK, et. al.
  • Organizer: American Heart Association Scientific Session2008.
  • Place of Presentation: New Orleans, LA, USA.
  • Year and Date: 2008-11-12
• [Presentation] A novel technology to prevent proarrhythmia of myoblast transplantation : "Patterning" of cell alignment using magnetic nanoparticles (2008)
  • Author(s): Takanari H, Lee, et al
  • Organizer: American Heart Association Scientific Session 2008
  • Place of Presentation: New Orleans, LA, USA
  • Year and Date: 2008-11-12