| Project/Area Number |
20590881
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Circulatory organs internal medicine
|
|---|
| Research Institution | Hamamatsu University School of Medicine |
|---|
Principal Investigator |
IHARA Hayato Hamamatsu University School of Medicine, 医学部, 助教 (00223298)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
北川 雅敏 浜松医科大学, 医学部, 教授 (50294971)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
YMAMOTO Seiji 浜松医科大学, 光量子医学研究センター, 准教授 (60144094)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | PAI-1,血管老化 / 細胞周期制御因子 / レスベラトロール / 血管内皮細胞 / PAI-1 / 細胞老化 / 老齢マウス / マイクロアレイ / PAI-1 KOマウス / 細胞周期関連因子 |
|---|
| Research Abstract |
To investigate the role of PAI-1 in vascular vessels senescence, we employed a vascular endothelial cell line, UV♀2 cells, for studying molecular mechanisms of senescence and PAI-1 KO mice for studying the links between senescence and aging in vivo. After confluent, UV♀2 cells increased expression levels of senescence-associated CDKI, p16^<INK4A> and a senescence marker, SA-β-gal staining. PAI-1 knockdown by RNA interference results in reduction of p16^<INK4A> gene expression in these cells. Treatments with resveratrol, an antioxidant polyphenol found in red wine, reduced p16^<INK4A> expression levels and SA-β-gal staining. DNA microarray analysis using aorta RNA from old-aged and young mice revealed that aging blood vessels increased expression levels of genes, such as PAI-1, MnSOD, Hsp etc. compared with young one and that aging blood vessels form PAI-1 KO mice reduced expression levels of genes concerned with cytoskeleton, extracellular matrix, mitochondrial components as compared with that from wild-type mice.
|
