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Enhancement of vascular vessels senescence caused by a fibrinolytic inhibitor, PAI-1.

Research Project

Project/Area Number 20590881
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

IHARA Hayato  Hamamatsu University School of Medicine, 医学部, 助教 (00223298)

Co-Investigator(Kenkyū-buntansha) 北川 雅敏  浜松医科大学, 医学部, 教授 (50294971)
Co-Investigator(Renkei-kenkyūsha) YMAMOTO Seiji  浜松医科大学, 光量子医学研究センター, 准教授 (60144094)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsPAI-1,血管老化 / 細胞周期制御因子 / レスベラトロール / 血管内皮細胞 / PAI-1 / 細胞老化 / 老齢マウス / マイクロアレイ / PAI-1 KOマウス / 細胞周期関連因子
Research Abstract

To investigate the role of PAI-1 in vascular vessels senescence, we employed a vascular endothelial cell line, UV♀2 cells, for studying molecular mechanisms of senescence and PAI-1 KO mice for studying the links between senescence and aging in vivo. After confluent, UV♀2 cells increased expression levels of senescence-associated CDKI, p16^<INK4A> and a senescence marker, SA-β-gal staining. PAI-1 knockdown by RNA interference results in reduction of p16^<INK4A> gene expression in these cells. Treatments with resveratrol, an antioxidant polyphenol found in red wine, reduced p16^<INK4A> expression levels and SA-β-gal staining. DNA microarray analysis using aorta RNA from old-aged and young mice revealed that aging blood vessels increased expression levels of genes, such as PAI-1, MnSOD, Hsp etc. compared with young one and that aging blood vessels form PAI-1 KO mice reduced expression levels of genes concerned with cytoskeleton, extracellular matrix, mitochondrial components as compared with that from wild-type mice.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 2008 Other

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (4 results) Remarks (1 results)

  • [Journal Article] Migration of mouse-induced pluripotent stem cells to glioma-conditioned medium is mediated by tumor-associated specific growth factors.2011

    • Author(s)
      Koizumi, S., Gu, C., Amano, S., Yamamoto, S., Ihara H., et.al.
    • Journal Title

      ONCOLOGY LETTERS 2

      Pages: 283-288

    • Related Report
      2010 Final Research Report
  • [Journal Article] Migration of mouse-induced pluripotent stem cells to glioma-conditioned medium is mediated by tumor-assOciated specific growth factors.2011

    • Author(s)
      Koizumi, S., et al.
    • Journal Title

      ONCOLOGY LETTERS

      Volume: 2 Pages: 283-288

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of platelet activation via P2Y12 receptor in the development of transplant arteriosclerosis in mice.2009

    • Author(s)
      Yashiro, K., Matsumoto, Y., Ihara H., et al.
    • Journal Title

      Transplantation 87

      Pages: 60-667

    • Related Report
      2010 Final Research Report
  • [Journal Article] Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vascular endothelial cells.2009

    • Author(s)
      Suzuki, Y., Mogami, H., Ihara H., Urano, T.
    • Journal Title

      Blood 113

      Pages: 470-478

    • NAID

      120005313162

    • Related Report
      2010 Final Research Report
  • [Journal Article] 機能性食品成分によるメタボリック症候群発症に関わるアディポカイン遺伝子発現抑制機構の解析-赤ワインに含まれるレスベラトロールの効果を中心として-2009

    • Author(s)
      井原勇人
    • Journal Title

      月刊フードリサーチ(特集号:メタボ対策とアンチエイジング) 645

      Pages: 22-25

    • Related Report
      2010 Final Research Report
  • [Journal Article] 脂肪細胞から分泌される生理活性物質アディポカイン遺伝子発現制御2009

    • Author(s)
      井原勇人
    • Journal Title

      比較生理生化学 26

      Pages: 47-57

    • NAID

      10025617430

    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Journal Article] Involvement of platelet activation via P2Y12 receptor in the development of transplant arteriosclerosis in mice.2009

    • Author(s)
      Yashiro, K., Matsumoto, Y., Ihara, H., et al.
    • Journal Title

      Transplantation 87(5)

      Pages: 660-667

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unique secretory dynamics of tissue plasminogen activator and its modulation by plasminogen activator inhibitor-1 in vaseular endothelial cells.2009

    • Author(s)
      Suzuki, Y., Mogami, H., Ihara, H., Urano, T.
    • Journal Title

      Blood 113

      Pages: 470-478

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 機能性食品成分によるメタボリック症候群発症に関わるアディポカイン遺伝子発現抑制機構の解析 - 赤ワインに含まれるレスペラトロールの効果を中心として-2009

    • Author(s)
      井原 勇人
    • Journal Title

      月刊 フードリサーチ(特集号 : メクボ対策とアンチエイジング) 645

      Pages: 22-25

    • Related Report
      2008 Annual Research Report
  • [Presentation] 赤ワインに含まれるレスベラトロールの抗肥満・抗老化効果2010

    • Author(s)
      井原勇人
    • Organizer
      日本技術士会食品技術士中部協議会講演
    • Place of Presentation
      名古屋
    • Year and Date
      2010-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 赤ワインに含まれるレスベラトロールの抗肥満・抗老化効果2010

    • Author(s)
      井原勇人
    • Organizer
      日本技術士会 食品技術士中部協議会
    • Place of Presentation
      名古屋
    • Year and Date
      2010-02-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] 機能性食品成分によるメタボリック症候群発症に関わるアディポカイン遺伝子発現抑制機構の解析-赤ワインに含まれるレスベラトロールの効果を中心として-2008

    • Author(s)
      井原勇人
    • Organizer
      日本技術士会食品技術士センター講演
    • Place of Presentation
      東京
    • Year and Date
      2008-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] 機能性食品成分によるメタボリック症候群発症に関わるアディポカイン遺伝子発現抑制機構の解析 -赤ワインに含まれるレスベラトロールの効果を中心として-2008

    • Author(s)
      井原 勇人
    • Organizer
      日本技術士会 食品技術士センター講演(依頼講演)
    • Place of Presentation
      東京
    • Year and Date
      2008-09-20
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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