Mechanisms in epithelial-mesenchymal transition in chronic obstructive pulmonary disease

• Project/Area Number: 20590896
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kyoto University
• Principal Investigator: ITO Isao Kyoto University, 医学研究科, 助教 (40447975)
• Co-Investigator(Kenkyū-buntansha): MISHIMA Michiaki 京都大学, 医学研究科呼吸器内科, 教授 (60190625) ; NIIMI Akio 京都大学, 医学研究科呼吸器内科, 准教授 (30252513) ; MURO Shigeo 京都大学, 医学研究科呼吸器内科, 助教 (60344454) ; HOSHINO Yuma 京都大学, 医学研究科呼吸器内科, 助教 (00378746)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: II型肺胞上皮細胞 / サーファクタントプロテインC / 上皮間葉移行 / COPD / 気管支喘息 / 気道上皮細胞 / 慢性閉塞性肺疾患 / 末梢気道

Research Abstract

By adding appropriate concentration of TGF-β to cultured BEAS-2B cell, the cells exhibited epithelial-mesenchymal transition, which were enhanced by addint TLR-ligands. To perform further experiments on EMT, induced pluripotent stem cells were established from patients with COPD and inducing type II pneumocytes from iPS cells is being tried.

Research Products

• [Journal Article] Eosinophils and macrophages are involved in nitrosative stress in chronic eosinophilic pneumonia. (2011)
  • Author(s): Nakaji H, Matsumoto H, Niimi A, Handa T, Ito I, Kanai M, Tabata H, Sakai H, Kobashi Y, Mochizuki Y, Chin K, Mishima M.
  • Journal Title: Nitric Oxide : Biology and Chemistry accepted
  • Peer Reviewed
• [Journal Article] Centrilobular opacities in the asthmatic lung successfully treated with inhaled ciclesonide and tiotropium : With assessment of alveolar nitric oxide levels. (2010)
  • Author(s): Kiyokawa H, Matsumoto H, Niimi A, Ito I, et al.
  • Journal Title: Allergol International Epub ahead of print
  • NAID: 130004723051
  • Peer Reviewed
• [Journal Article] Distribution and clonal relationship of cell surface virulence genes among Streptococcus pneumoniae isolates in Japan. (2010)
  • Author(s): Imai S, Ito Y, Ishida T, Hirai T, Ito I, Yoshimura K, Maekawa K, Takakura S, Niimi A, Iinuma Y, Ichiyama S, Mishima M ; the Kansai Community Acquired Pneumococcal Pneumonia Study Group.
  • Journal Title: Clin Microbiol Infect. Epub ahead of print
  • Peer Reviewed
• [Journal Article] Association of alveolar nitric oxide levels with pulmonary function and its reversibility in stable asthma. (2010)
  • Author(s): Matsumoto H, Niimi A, Jinnai M, Nakaji H, Takeda T, Oguma T, Otsuka K, Inoue H, Yamaguchi M, Matsuoka H, Ito I, Hirai T, Chin K, Mishima M.
  • Journal Title: Respiration Epub ahead of print
  • NAID: 120005593637
  • Peer Reviewed
• [Journal Article] Desert-dust exposure is associated with increased risk of asthma hospitalization in children. (2010)
  • Author(s): Kanatani K, Ito I, Al-Delaimy WK, Adachi Y, Mathews WC, Ramsdell JW, Toyama Asian Desert Dust, Asthma Study Team.
  • Journal Title: Am J Respir Crit Care Med 182 Pages: 1475-81
  • NAID: 120005593569
  • Peer Reviewed
• [Journal Article] Inflammatory subtypes in cough variant asthma : Association with maintenance doses of inhaled corticosteroids. (2010)
  • Author(s): Matsuoka H, Niimi A, Matsumoto H, Takemura M, Ueda T, Yamaguchi M, Jinnai M, Inoue H, Ito I, Chin K, Mishima M.
  • Journal Title: Chest 138 Pages: 1418-25
  • Peer Reviewed
• [Journal Article] Tazobactam/piperacillin for moderate-to-severe pneumonia in patients with risk for aspiration : comparison with imipenem/cilastatin. (2010)
  • Author(s): Ito I, Kadowaki S, Tanabe N, Haruna A, Kase M, Yasutomo Y, Tsukino M, Nakai A, Matsumoto H, Niimi A, Chin K, Ichiyama S, Mishima M.
  • Journal Title: Pulm Pharmacol Ther 23 Pages: 403-10
  • NAID: 120002484950
  • Peer Reviewed
• [Journal Article] Lipopolysaccharide induced connective tissue growth factor gene expression in human bronchial epithelial cells. (2010)
  • Author(s): Nishioka M, Ogawa E, Kinose D, Haruna A, Ohara T, Ito I, Hoshino Y, Ito Y, Matsumoto H, Niimi A, Mio T, Chin K, Hirai T, Muro S, Mishima M.
  • Journal Title: Respirology 15 Pages: 669-76
  • Peer Reviewed
• [Journal Article] Induced sputum concentrations of mucin in patients with asthma and chronic cough. (2010)
  • Author(s): Jinnai M, Niimi A, Ueda T, Matsuoka H, Takemura M, Yamaguchi M, Otsuka K, Oguma T, Takeda T, Ito I, Matsumoto H, Mishima M.
  • Journal Title: Chest 137 Pages: 1122-9
  • Peer Reviewed
• [Journal Article] Molecular characterization of quinolone resistance-determining regions and their correlation with serotypes and genotypes among Streptococcus pneumoniae isolates in Japan. (2010)
  • Author(s): Osawa M, Ito Y, Ishida T, Imai S, Ichiyama S, Mishima M ; Kansai Community Acquired Pneumococcal Pneumonia Study Group.
  • Journal Title: Eur J Clin Microbiol Infect Dis 29 Pages: 245-8
  • Peer Reviewed
• [Journal Article] Relationship between Small Airway Function and Health Status, Dyspnea and Disease Control in Asthma. (2010)
  • Author(s): Takeda T, Oga T, Niimi A, Matsumoto H, Ito I, Yamaguchi M, Matsuoka H, Jinnai M, Otsuka K, Oguma T, Nakaji H, Chin K, Mishima M.
  • Journal Title: Respiration 80 Pages: 120-6
  • Peer Reviewed
• [Journal Article] Asian Desert Dust and Asthma Study Team. Desert-dust exposure is associated with increased risk of asthma hospitalization in children (2010)
  • Author(s): Kanatani KT
  • Journal Title: Am J Respir Crit Care Med Volume: 182 Pages: 1475-81
  • Peer Reviewed
• [Journal Article] Tazobactam/piperacillin for moderate-to-severe pneumonia in patients with risk for aspiration : comparison with imipenem/cilastatin (2010)
  • Author(s): Ito I
  • Journal Title: Pulm Pharmacol Ther Volume: 23 Pages: 403-10
  • NAID: 120002484950
  • Peer Reviewed
• [Journal Article] High prevalence of multidrug-resistant Pneumococcal molecular epidemiology network clones among Streptococcus pneumoniae isolates from adult patients with community-acquired pneumonia in Japan. (2009)
  • Author(s): Imai S, Ito Y, Ishida T, Hirai T, Ito I, Maekawa K, Takakura S, Iinuma Y, Ichiyama S, Mishima M, Kansai Community Acquired Pneumococcal Pneumonia Study Group.
  • Journal Title: Clin Microbiol Infect 15 Pages: 1039-45
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor and transforming growth factor-beta modulate the expression of matrix metalloproteinases and migratory function of human airway smooth muscle cells. (2009)
  • Author(s): Ito I, Fixman ED, Asai K, Yoshida M, Gounni AS, Martin JG, Hamid Q.
  • Journal Title: Clin Exp Allergy 39 Pages: 1370-80
  • Peer Reviewed
• [Journal Article] Effect of inhaled corticosteroids on small airways in asthma : investigation using impulse oscillometry. (2009)
  • Author(s): Yamaguchi M, Niimi A, Ueda T, Takemura M, Matsuoka H, Jinnai M, Otsuka K, Oguma T, Takeda T, Ito I, Matsumoto H, Hirai T, Chin K, Mishima M.
  • Journal Title: Pulm Pharmacol Ther 26 Pages: 326-32
  • Peer Reviewed
• [Journal Article] Specific IgE response to Trichophyton and asthma severity. (2009)
  • Author(s): Matsuoka H, Niimi A, Matsumoto H, Ueda T, Takemura M, Yamaguchi M, Jinnai M, Otsuka K, Oguma T, Takeda T, Ito I, Chin K, Amitani R, Mishima M.
  • Journal Title: Chest 135 Pages: 898-903
  • Peer Reviewed
• [Journal Article] Differentiation of bacterial and non-bacterial community-acquired pneumonia by thin-section computed tomography. (2009)
  • Author(s): Ito I, Ishida T, Togashi K, Niimi A, Koyama H, Ishimori T, Kobayashi H, Mishima M.
  • Journal Title: Eur J Radiol 72 Pages: 388-95
  • NAID: 120002576696
  • Peer Reviewed
• [Journal Article] Platelet-derived growth factor and transforming growth factor-beta modulate the expression of matrix metalloproteinases and migratory function of human airway smooth muscle cells. (2009)
  • Author(s): Isao Ito
  • Journal Title: Clin Exp Allergy 15 Pages: 1039-1045
  • Peer Reviewed
• [Journal Article] Molecular characteristics of serotype 3 Streptococcus pneumoniae isolates among community-acquired pneumonia patients in Japan. (2008)
  • Author(s): Isozumi R, Ito Y, Ishida T, Hirai T, Ito I, Maniwa K, Hayashi M, Kagioka H, Hirabayashi M, Onaru K, Tomioka H, Tomii K, Gohma I, Osawa M, Imai S, Takakura S, Iinuma Y, Chin K, Ichiyama S, Mishima M ; the Kansai Community Acquired Pneumococcal Pneumonia Study Group.
  • Journal Title: J Infect Chemother 14 Pages: 258-61
  • NAID: 10022595304
  • Peer Reviewed
• [Journal Article] Sputum levels of transforming growth factor-b1 in asthma : Relation to clinicoradiological findings. (2008)
  • Author(s): Yamaguchi M, Niimi A, Matsumoto H, Ueda T, Takemura M, Matsuoka H, Jinnai M, Otsuka K, Oguma T, Takeda T, Ito I, Chin K, Mishima M.
  • Journal Title: J Investig Allergol Clin Immunol 18 Pages: 202-6
  • Peer Reviewed
• [Journal Article] Antimicrobial susceptibilities of Streptococcus pneumoniae isolated from adult patients with community-acquired pneumonia in Japan. (2008)
  • Author(s): Ishida T, Maniwa K, Kagioka H, Hirabayashi M, Onaru K, Tomioka H, Hayashi M, Tomii K, Gohma I, Ito Y, Hirai T, Ito I, Mishima M.
  • Journal Title: Respirology 13 Pages: 240-6
  • Peer Reviewed
• [Journal Article] PDGF and TGF-b modulate expression of MMPs and migratory function in human airway smooth muscle cells
  • Author(s): Isao Ito
  • Journal Title: Clinical Experimental Allergy
  • Peer Reviewed
• [Presentation] 安定期喘息患者における誘発喀痰中好酸球比率の臨床的意義 (2010)
  • Author(s): 井上英樹
  • Organizer: 第22回日本アレギー学会春季臨床大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] 児における黄砂飛来と喘息発作の関係-ケースクロスオーバースタディによる評価 (2010)
  • Author(s): 金谷久美子
  • Organizer: 第22回日本アレルギー学会春季臨床大会
  • Place of Presentation: 京都(招待講演)
• [Presentation] 黄砂飛来時の小児喘息入院リスク上昇は花粉予報時に低減:富山でのCase-Crossover Study (2010)
  • Author(s): 金谷久美子
  • Organizer: 第60回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京(招待講演)
• [Presentation] シンポジウム7.「呼吸器専門医の在り方と制度」大学病院における専門医のあり方(理想像) (2009)
  • Author(s): 伊藤功朗
  • Organizer: 第49回日本呼吸器学会総会
  • Place of Presentation: 東京
• [Presentation] MMP-9プロモータ遺伝子多型は上肺野優位の肺気腫病変と関連する (2009)
  • Author(s): 伊藤功朗, 長井苑子, 半田知宏, 室繁郎, 平井豊博, 月野光博, 三嶋理晃
  • Organizer: 第49回日本呼吸器学会総会
  • Place of Presentation: 東京
• [Presentation] HRCTを用いた細菌性肺炎と非定型肺炎の鑑別に関するprospective study (2009)
  • Author(s): 伊藤功朗, 石田直, 橋本徹, 有田真知子, 大澤真, 橘洋正, 新実彰男, 三嶋理晃
  • Organizer: 第49回日本呼吸器学会総会
  • Place of Presentation: 東京
• [Presentation] TNF-alpha Modulates Expressions of MMPs and Migratory Function of Human Airway Smooth Muscle Cells. (2008)
  • Author(s): 伊藤功朗, et al.
  • Organizer: 2008 ATS International Conference
  • Place of Presentation: Toronto
• [Presentation] TNF-αによるヒト気道平滑筋細胞MMP発現と細胞遊走への影響 (2008)
  • Author(s): 伊藤功朗, ら
  • Organizer: 第58回日本アレルギー学会秋季学術大会
  • Place of Presentation: 東京
• [Book] 京都大学医学部附属病院市中肺炎・院内肺炎治療マニュアル(三嶋理晃・一山智監修.ライフ・サイエンス) (2010)
  • Author(s): 伊藤功朗著
  • Total Pages: 99
• [Book] 京都大学医学部附属病院市中肺炎・院内肺炎治療マニュアル (2010)
  • Author(s): 伊藤功朗
  • Total Pages: 99
  • Publisher: ライフ・サイエンス