Functional analysis of cancer stem cell/side population in non-small lung cancer cells

• Project/Area Number: 20590918
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Nagoya University
• Principal Investigator: KONDO Masashi Nagoya University, 医学部附属病院, 講師 (00378077)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
• Keywords: EGFR / 癌幹細胞 / 非小細胞肺癌 / 肺癌 / 癌幹細 / EGFR遺伝子変異

Research Abstract

Functional analysis of cancer stem cell is very important to establish the method of early detection and/or effective therapy for the non-small cell lung cancer since stem cells seem to be involved carcinogenesis and the resistance for the chemotherapy. In this study, we try to identify the cell populations that have cancer stem cell property and important molecule related with stem cells. We fund that the formation of sphere in serum free and anchorage-dependent culture condition was promising for the selection of stem cells population, and that epithelial cell adhesion molecule (EpCAM) play the important roll in survival of cancer cells.

Research Products

• [Journal Article] Taniguchi H Second-line weekly paclitaxel in resistant or relapsed non-small cell lung cancer treated with docetaxel and carboplatin : a multi-center phase II study. (2010)
  • Author(s): Ichikawa M, Suzuki R, Kataoka K, Noda Y, Shindoh J, Matsumoto S, Tanikawa Y, Suzuki K, Baba K, Shindo Y, Kondo M, Imaizumi K, Kume H, Hasegawa Y, Takagi K
  • Journal Title: Lung Cancer. 69(3) Pages: 319-322
  • Peer Reviewed
• [Journal Article] Phase II study of carboplatin and gemcitabine as adjuvant chemotherapy in patients with completely resected non-small cell lung cancer : a report from the Central Japan Lung Study Group, CJLSG 0503 trial. (2010)
  • Author(s): Usami N, Yokoi K, Hasegawa Y, Taniguchi H, Shindo J, Yamamoto M, Suzuki R, Imaizumi K, Kondo M, Shimokata K
  • Journal Title: Int J Clin Oncol. 15 Pages: 583-587
  • NAID: 10027665356
  • Peer Reviewed
• [Journal Article] Prospective evaluation of pharmacokinetically guideddosing of carboplatin in Japanese patients with cancer. (2010)
  • Author(s): Shimokata T, Ando Y, Yasuda Y, Hamada A, Kawada K, Saito H, Matsuo S, Kondo M, Imaizumi K, Hasegawa Y.
  • Journal Title: Cancer Sci. Cancer Sci. 101 Pages: 2601-2605
  • Peer Reviewed
• [Journal Article] Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells. (2010)
  • Author(s): Takeyama Y, Sato M, Horio M, Hase T, Yoshida K, Yokoyama T, Nakashima H, Hashimoto N, Sekido Y, Gazdar AF, Minna JD, Kondo M, Hasegawa Y.
  • Journal Title: Cancer Lett. 296 Pages: 216-224
  • Peer Reviewed
• [Journal Article] Knockdown of ZEB1, a master epithelial-to-mesenchymal transition (EMT) gene, suppresses anchorage-independent cell growth of lung cancer cells. (2010)
  • Author(s): Takeyama Y, Kondo M, et al.
  • Journal Title: Cancer Lett. Volume: 2897 Pages: 216-224
  • Peer Reviewed
• [Journal Article] Epigenetic profiles distinguish malignant pleural mesothelioma from lung adenocarcinoma. (2009)
  • Author(s): Goto Y, Shinjo K, Kondo Y, Shen L, Toyota M, Suzuki H, Gao W, An B, Fujii M, Murakami H, Osada H, Taniguchi T, Usami N, Kondo M, Hasegawa Y, Shimokata K, Matsuo K, Hida T, Fujimoto N, Kishimoto T, Issa JP, Sekido Y.
  • Journal Title: Cancer Res. 69 Pages: 9073-9082
  • Peer Reviewed
• [Journal Article] 遺伝子多型、遺伝子変異と肺癌の個別化医療 (2009)
  • Author(s): 近藤征史、堀尾美穂子、長谷川好規
  • Journal Title: 治療学 43 Pages: 46-50
• [Presentation] EMT制御因子ZEB1のノックダウンは肺がん細胞の足場非依存性増殖を抑制する (2010)
  • Author(s): 竹山佳宏, 佐藤光夫, 堀尾美穂子, 長谷哲成, 吉田健也, 小栗知世, 関戸好孝, 近藤征史, 長谷川好規
  • Organizer: 第51回日本肺癌学会
  • Place of Presentation: 広島
  • Year and Date: 2010-11-04
• [Presentation] 腫瘍EMT制御因子ZEB1のノックダウンは肺癌細胞の増殖を抑制する (2010)
  • Author(s): 竹山佳宏, 佐藤光夫, 堀尾美穂子, 長谷哲成, 吉田健也, 小栗知世, 関戸好孝, 近藤征史, 長谷川好規
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-24
• [Presentation] 腫瘍EMT制御因子ZEB1のノックダウンは肺癌細胞の増殖を抑制する(会議録) (2010)
  • Author(s): 竹山佳宏, 近藤征史, ら
  • Organizer: 第50回日本呼吸器学会学術講演会
  • Place of Presentation: 京都
  • Year and Date: 2010-04-24
• [Presentation] Knockdown of ZEB1, a master epithelial mesenchymal transition (EMT) inducing gene, suppresses growth of pleural mesothelioma cell lines (2010)
  • Author(s): Mihoko Horio, Mitsuo Sato1, Yoshihiro Takeyama, Tetsunari Hase1, Kenya Yoshida1,Yoshitaka Sekido, Masashi Kondo, Yoshinori Hasegawa
  • Organizer: アメリカ癌学会年次総会
  • Place of Presentation: ワシントン
  • Year and Date: 2010-04-19
• [Presentation] Knockdown of ZEB1, a master EMT inducing gene, suppresses, anchorage-independent cell growth of lung cancer cells (2010)
  • Author(s): Mitsuo Sato , Yoshihiro Takeyama , Mihoko Horio, Tetsunari Hase, Kenya Yoshida1,Harunori Nakashima, Naozumi Hashimoto, Yoshitaka Sekido, Masashi Kondo, Yoshinori Hasegawa
  • Organizer: アメリカ癌学会年次総会
  • Place of Presentation: ワシントン
  • Year and Date: 2010-04-19
• [Presentation] ゲフィチニブの有害事象とABCトランスポーターの遺伝子多型の相関 (2008)
  • Author(s): 田村真理子, 近藤征史, ら
  • Organizer: 日本肺癌学会総会
  • Place of Presentation: 北九州市西日本総合展示場
  • Year and Date: 2008-11-13