• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Fundamental study of development of new antibiotics using defensins

Research Project

Project/Area Number 20590927
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionNagasaki University

Principal Investigator

MUKAE Hiroshi  Nagasaki University, 医学部, 教授 (80253821)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsデフェンシン / プレクタシン / サイトカイン / 増殖因子 / 気道上皮細胞 / 抗菌活性 / 炎症性腸疾患 / 肺線維化 / 肺線維芽細胞 / αデフェンシン / Plectasin / ムチン産生
Research Abstract

1. Plectasin is the first defensin-like peptide to be isolated from fungus, which has specific antibacterial activity against Streptococcus pneumoniae. In addition, it showed no cytotoxicity to human cells, and it did not induce IL-8 production in A549 cells. This suggests that plectasin could be an inoffensive alternative antibiotic for clinical application. 2. Human α-defensins contribute to excessive mucus production by increasing the production of MUC5AC from bronchial epithelial cells. Macrolide antibiotics directly inhibit these actions. 3. Human α-defensins mainly enhanced the expression of IL-8 in epithelial cells, whereas it enhanced transforming growth factor-β and vascular endothelial growth factor expressions in lung fibroblasts. These results suggest that humanα-defensins play different roles in the pathogenesis of idiopathic pulmonary fibrosis and diffuse panbronchiolitis according to the location in the lung where the humanα-defensins are mainly produced. It is possible that these effects of humanα-defensins carry a risk of adverse effects. Therefore we should examine the possibility of fungal defensins like plectasin as antibiotics.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (22 results)

All 2010 2009 2008

All Journal Article (18 results) (of which Peer Reviewed: 11 results) Presentation (3 results) Book (1 results)

  • [Journal Article] Differential effects of human neutrophil peptide-1 on growth factor and interleukin-8 production by human lung fibroblasts and epithelial cells2010

    • Author(s)
      Amenomori M, Mukae H, Ishimatsu Y, Sakamoto N, Kakugawa T, Hara A, Hara S, Fujita H, Ishimoto H, Hayashi T, Kohno S
    • Journal Title

      Exp Lung Res 36巻

      Pages: 411-419

    • NAID

      120006984965

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] デフェンシンと呼吸器疾患特集II自然免疫と呼吸器疾患2010

    • Author(s)
      迎寛
    • Journal Title

      炎症と免疫 18巻

      Pages: 62-365

    • Related Report
      2010 Final Research Report
  • [Journal Article] 「臨床に貢献できる研究」を目指して2010

    • Author(s)
      迎寛
    • Journal Title

      呼吸 29巻

      Pages: 113-114

    • Related Report
      2010 Final Research Report
  • [Journal Article] Differential effects of human neutrophil peptide-1 on growth factor and interleukin-8 production by human lung fibroblasts and epithelialcells2010

    • Author(s)
      Amenomori M, Mukae H, et al.
    • Journal Title

      Exp Lung Res

      Volume: 36 Pages: 411-419

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] デフェンシンと呼吸器疾患 特集II 自然免疫と呼吸器疾患2010

    • Author(s)
      迎寛
    • Journal Title

      炎症と免疫

      Volume: 18 Pages: 362-365

    • Related Report
      2010 Annual Research Report
  • [Journal Article] 「臨床に貢献できる研究」を目指して2010

    • Author(s)
      迎寛
    • Journal Title

      呼吸

      Volume: 29 Pages: 113-114

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Differential effects of human neutrophil peptide-1 on growth factor and IL-8 production by human lung fibroblasts and epithelial cells2010

    • Author(s)
      Amenomori M, Mukae H, et al.
    • Journal Title

      Exp Lung Res (印刷中)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Concentrations of α-and β-defensins in plasma of patients with inflammatory bowel disease2009

    • Author(s)
      Yamaguchi N, Isomoto H, Mukae H, Ishimoto H, Ohnita K, Shikuwa S, Mizuta Y, Nakazato M, Kohno S
    • Journal Title

      Inflamm Res 58巻

      Pages: 192-197

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 呼吸器感染症とデフェンシン2009

    • Author(s)
      迎寛
    • Journal Title

      臨床と研究 85巻

      Pages: 246-247

    • Related Report
      2010 Final Research Report
  • [Journal Article] Different effects of telithromycin on MUC5AC production induced by human neutrophil peptide-1 or lipopolysaccharide in NCI-H292 cells compared with azithromycin and clarithromycin2009

    • Author(s)
      Ishimoto H, Mukae H, Sakamoto N, Amenomori M, Kitazaki T, Imamura Y, Fujita H, Ishii H, Nakayama S, Yanagihara K, Kohno S
    • Journal Title

      J Antimicrob Chemother 63巻

      Pages: 109-114

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Concentrations of alpha- and beta-defensins in plasma of patients with inflammatory bowel disease2009

    • Author(s)
      Yamaguchi N, Mukae H, et al.
    • Journal Title

      Inflamm Res 58

      Pages: 192-197

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 抗菌ペプチド(デフェンシン)2009

    • Author(s)
      迎寛
    • Journal Title

      Anllual Review呼吸器2009

      Pages: 27-32

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Different effects of telithromycin on MUC5AC production induced by human neutrophil peptide -1 or lipopolysaccharide in NCI -H292 cells compared with azithromycin and clarithromycin2009

    • Author(s)
      Ishimoto H, Mukae H, et al.
    • Journal Title

      J Antimicrob Chemother 63

      Pages: 109-114

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] デフェンシンと呼吸器感染症2008

    • Author(s)
      迎寛
    • Journal Title

      呼吸 27巻

      Pages: 24-28

    • Related Report
      2010 Final Research Report
  • [Journal Article] Plectasin has antimicrobial activity and no affect on cell viability or IL-8 production2008

    • Author(s)
      Hara S, Mukae H, Sakamoto N, Ishimoto H, Amenomori M, Fujita H, Ishimatsu Y, Yanagihara K, Kohno S
    • Journal Title

      Biochem Biophys Res Commun 374巻

      Pages: 709-713

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] デフェンシンと肺の感染防御、炎症、線維化2008

    • Author(s)
      迎寛
    • Journal Title

      日本肺サーファクタント・界面医学会雑誌 39巻

      Pages: 9-11

    • NAID

      10021919688

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Plectasin has antimicrobial activity and no affect on cell viability or IL -8 production2008

    • Author(s)
      Hara S, Mukae H, et al.
    • Journal Title

      Biochem Biophy Res Com 374

      Pages: 709-713

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] デフェンシンと肺の感染防御、炎症、線維化2008

    • Author(s)
      迎 寛
    • Journal Title

      日本肺サーファクタント・界面医学会雑誌 39

      Pages: 9-11

    • NAID

      10021919688

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Plectasin~新規抗菌薬としての臨床応用可能性に関する検討~2008

    • Author(s)
      原信太郎、迎寛、他
    • Organizer
      第78回日本感染症学会西日本地方会学術集会
    • Place of Presentation
      広島
    • Year and Date
      2008-12-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Plectasin has antibacterial activity and no affect on cell viability or IL-8 production2008

    • Author(s)
      Hara S, Mukae H, et al
    • Organizer
      13th Congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2008-11-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Plectasin has antibacterial activity and no affect on cell viability or IL -8 production2008

    • Author(s)
      Hara S, Mukae H, et al.
    • Organizer
      13th Congress of the Asian Pacific Society of Respirology
    • Place of Presentation
      Bangkok, Thailand
    • Year and Date
      2008-11-20
    • Related Report
      2008 Annual Research Report
  • [Book] 抗菌ペプチド(デフェンシン)、Annual Review呼吸器2009

    • Author(s)
      迎寛
    • Publisher
      中外医学社
    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi