TLR gene expression in e ndotoxin-induced lung injury

• Project/Area Number: 20590932
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: IWASAKI Yoshinobu Kyoto Prefectural University of Medicine, 医学研究科, 准教授 (00203373)
• Co-Investigator(Kenkyū-buntansha): MARUNAKA Yoshinori 京都府立医科大学, 医学研究科, 教授 (00127036) ; NIISATO Naomi 京都府立医科大学, 医学研究科, 准教授 (00237645) ; ARIMOTO Taichiro 京都府立医科大学, 医学研究科, 講師 (30457965) ; UEDA Mikio 京都府立医科大学, 医学研究科, 助教 (40457966) ; HOSOGI Shigekuni 京都府立医科大学, 医学研究科, 助教 (30433254) ; 細木 誠之 京都府立医科大学, 医学研究科, 助教 (30405261)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: TLR / エンドトキシン肺損傷 / CD14 / 肺胞上皮 / アポトーシス / シグナリングアッセイ / 肺水腫

Research Abstract

In the experimental model of endotoxin-induced acute lung injury using rats, TLR4 gene and protein expression were found followed by apoptosis of alveolar epithelia. Soluble CD14 accelerated apoptosis of alveolar epithelia by activating TLR4. Inactivation of soluble CD14 reduced alveolar epithelial damage.

Research Products

• [Journal Article] Intracellular Chloride Regulates Cell Proliferation Through the Activation of Stress-Activated Protein Kinases (2010)
  • Author(s): OHSAWA RUMI, MIYAZAKI HIROAKI, NIISATO NAOMI, SHIOZAKI ATSUSHI, IWASAKI YOSHINOBU, OTSUJI EIGO, MARUNAKA YOSHINORI
  • Journal Title: MKN28 Human Gastric Cancer Cells Journal of Cellular Physiology 223 Pages: 764-770
  • Peer Reviewed
• [Journal Article] Chloride Ion Modulates Cell Proliferation of Human Androgen-Independent Prostatic Cancer Cell (2010)
  • Author(s): HIRAOKA KENJI, MIYAZAKI HIROAKI, NIISATO NAOMI, IWASAKI YOSHINOBU, KAWAUCHI AKIHIRO, MIKI TSUNEHARU, MARUNAKA YOSHINORI
  • Journal Title: Cellular Physiology 25 Pages: 379-388
  • Peer Reviewed
• [Journal Article] Intracellular Chloride Regulates Cell Proliferation Through the Activation of Stress-Activated Protein Kinases in MKN28 Human Gastric Cancer Cells (2010)
  • Author(s): OHSAWA RUMI
  • Journal Title: Journal of Cellular Physiology Volume: 223 Pages: 764-770
  • Peer Reviewed
• [Journal Article] Chloride Ion Modulates Cell Proliferation of Human Androgen-Independent Prostatic Cancer Cell (2010)
  • Author(s): HIRAOKA KENJI
  • Journal Title: Cellular Physiology Volume: 25 Pages: 379-388
  • Peer Reviewed
• [Journal Article] Action of N-acylated ambroxol derivatives on secretion of chloride ions (2009)
  • Author(s): YAMADA TAKAO, TAKEMURA YOSHIZUMI, NIISATO NAOMI, MITSUYAMA E, IWASAKI YOSHINOBU
  • Journal Title: hyuman airway epithelia 380 Pages: 586-590
  • Peer Reviewed
• [Journal Article] Quercetin Stimulates Na1/K1/2Cl2 Cotransport via PTK-Dependent Mechanism (2009)
  • Author(s): ASANO JUNJI, NIISATO NAOMI, NAKAJIMA KEN-ICHI, MIYAZAKI HIROAKI, YASUDA MAKOTO, IWASAKI YOSHINOBU, HAMA TAKEMITSU, DEJIMA KENJI, MARUNAKA YOSHINORI
  • Journal Title: Human Airway Epithelium 41 Pages: 688-695
  • Peer Reviewed
• [Journal Article] Phase II trial of weekly gemcitabine and split-dose cisplatin for advanced non-small-cell lung cancer. (2009)
  • Author(s): HIRAMATSU ATSUSHI, IWASAKI YOSHINOBU, KOYAMA YASUNORI, TAMIYA NOBUYO, HOSOGI SHIGEKUNI, NAKANISHI MASAKI, KOHNO YOSHIHITO, UEDA MIKIO, ARIMOTO TAICHIRO, MARUNAKA YOSHINORI
  • Journal Title: Jpn J Clin Oncol 39(12) Pages: 779-783
  • Peer Reviewed
• [Journal Article] Action of N-acylated ambroxol derivatives on secretion of chloride ions in human airway epithelia (2009)
  • Author(s): YAMADA TAKAO
  • Journal Title: Biochem Biophys Res Commun 380 Pages: 586-590
  • Peer Reviewed
• [Journal Article] Quercetin Stimulates Na1/K1/2C12 Cotransport via PTK-Dependent Mechanisms in Human Airway Epithelium (2009)
  • Author(s): ASANO JUNJI
  • Journal Title: Am J Respir Cell Mol Biol 41 Pages: 688-695
  • Peer Reviewed
• [Journal Article] Effect of inducible nitric oxide synthase on apoptosis (2008)
  • Author(s): HOSOGI SHIGEKUNI, IWASAKI YOSHINOBU, YAMADA TAKAHIRO, KOMATANI-TAMIYA NOBUYO, HIRAMATSU ATSUSHI, KOHNO YOSHIHITO, UEDA MIKIO, ARIMOTO TAICHIRO, MARUNAKA YOSHINORI
  • Journal Title: Candida-induced acute lung injury 29 Pages: 257-266
  • Peer Reviewed
• [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulating the expression of p21 through a p53-independent pathway (2008)
  • Author(s): MIYAZAKI HIROAKI, SHIOZAKI ATSUSHI, NIISATA NAOMI, OHSAWA RUMI, ITOI H, UEDA Y, OTSUJI EIGO, YAMAGISHI HISAKAZU, IWASAKI YOSHINOBU, NAKANO T, NAKAHARI TAKASHI, MARUNAKA YOSHINORI
  • Journal Title: human gastric cancer cells 66 Pages: 7-7
  • Peer Reviewed
• [Journal Article] Effect of inducible nitric oxide synthase on apoptosis in Candida-induced acute lung iniury. (2008)
  • Author(s): HOSOGI SHIGEKUNI
  • Journal Title: Biomedical Research 29・5 Pages: 10-10
  • Peer Reviewed
• [Journal Article] Chloride ions control the G1/S cell-cycle checkpoint by regulatingthe expression of p21 through a p53-independent pathway in humangastric cancer cells. (2008)
  • Author(s): MIYAZAKI HIROAKI
  • Journal Title: Biochemical and Biophysical Research Communications 366・2 Pages: 7-7
  • Peer Reviewed