Functional analysis of Mycoplasma pneumoniae-derived lipoproteins in pneumonia onset

• Project/Area Number: 20590938
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Respiratory organ internal medicine
• Research Institution: Kurume University
• Principal Investigator: KUWANO Koichi Kurume University, 医学部, 教授 (60215118)
• Co-Investigator(Kenkyū-buntansha): SHIMIZU Takashi 久留米大学, 医学部, 助教 (40320155) ; KIDA Yutaka 久留米大学, 医学部, 講師 (30309752)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 感染症 / 肺炎 / マイコプラズマ / 炎症 / リポプロテイン / TLR / Mycoplasma pneumoniae / 抗体 / サイトカイン / ELISA / 炎症性サイトカイン / ウエスタンブロット / アミノ酸 / FcR

Research Abstract

Mycoplasma pneumoniae is known to cause pneumonia in humans. It is, however, unclear how pneumonia occurs. Our laboratory previously reported that Mycoplasma pneumoniae-derived lipoproteins induce inflammatory responses. We then examined the interaction between the lipoproteins and host in order to elucidate the onset mechanisms. We found that the lipoproteins are extremely important in the onset of mycoplasma-pneumonia.

Research Products

• [Journal Article] Cooperation between LepA and PlcH contributes to the in vivo virulence and growth of Pseudomonas aeruginosa in mice. (2011)
  • Author(s): Kida Y, Shimizu T, Kuwano K.
  • Journal Title: Infect Immun. 79 Pages: 211-219
  • Peer Reviewed
• [Journal Article] Cytoadherence-dependent induction of inflammatory responses by Mycoplasma pneumoniae. (2011)
  • Author(s): Shimizu T, Kida Y, Kuwano K
  • Journal Title: Immunology 133 Pages: 51-61
  • NAID: 10029813402
  • Peer Reviewed
• [Journal Article] Modifications on amphiphilicity and cationicity of unnatural amino acid containing peptides for the improvement of antimicrobial activity against pathogenic bacteria (2010)
  • Author(s): Taira J、Kida Y、Yamaguchi H、 Kuwano K、Higashimoto Y、Kodama H
  • Journal Title: Journal of peptide science 16 Pages: 607-612
  • Peer Reviewed
• [Journal Article] ナイシンAを利用した酪農分野における牛感染症防除 (2010)
  • Author(s): 北崎宏平、馬場武志、古賀康弘、桑野剛一
  • Journal Title: FFIジャーナル 215 Pages: 449-456
  • Peer Reviewed
• [Journal Article] Mycoplasma pneumoniaeの接着に依存的な炎症誘導 (2010)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Journal Title: 日本マイコプラズマ学会雑誌 Volume: 37(印刷中)
  • NAID: 10029813402
• [Journal Article] ナイシンAを利用した酪農分野における牛感染症防除 (2010)
  • Author(s): 北崎宏平、馬場武史、古賀康弘、桑野剛一, その他
  • Journal Title: Foods and Food Ingredients Journal of Japan Volume: 215 Pages: 449-456
  • Peer Reviewed
• [Journal Article] Mycoplasma pneumoniae リポプロテインを標的抗原としたELISAの構築 (2010)
  • Author(s): 桑野剛一、清水隆
  • Journal Title: 日本マイコプラズマ学会雑誌 36(印刷中)
  • NAID: 10026388696
• [Journal Article] 接着に依存的な Mycoplasma pneumoniae の炎症誘導 (2010)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Journal Title: 日本マイコプラズマ学会雑誌 36(印刷中)
  • NAID: 10026388708
• [Journal Article] Mycoplasma pneumoniae リポプロテインを標的抗原としたELISA の構築 (2009)
  • Author(s): 桑野剛一、清水隆
  • Journal Title: 日本マイコプラズマ学会雑誌 36 Pages: 4-5
  • NAID: 10026388696
• [Journal Article] 接着に依存的なMycoplasma pneumoniaeの炎症誘導 (2009)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Journal Title: 日本マイコプラズマ学会雑誌 36 Pages: 13-15
  • NAID: 10026388708
• [Journal Article] Mycoplasma pneumoniae-derived Lipopeptides Induce Acute Inflammatory Responses in the Lungs of Mice. (2008)
  • Author(s): Shimizu T, Kida Y, Kuwano K
  • Journal Title: Infect Immun. 76 Pages: 270-277
  • Peer Reviewed
• [Journal Article] A novel secreted protease from Pseudomonas aeruginosa activates NF-kappaB through protease-activated receptors. (2008)
  • Author(s): Kida Y, Higashimoto Y, Inoue H, Shimizu T, Kuwano K
  • Journal Title: Cell Microbiol. 10 Pages: 1491-1504
  • Peer Reviewed
• [Journal Article] Ureaplasma parvum lipoproteins, including MB antigen, activate NF-kappaB through TLR1, TLR2 and TLR6 (2008)
  • Author(s): Shimizu T, Kida Y, Kuwano K
  • Journal Title: Microbiology 154 Pages: 1318-1325
  • Peer Reviewed
• [Journal Article] Ureaplasma parvum由来リポタンパク質によるToll-like receptorシグナルの活性化 (2008)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Journal Title: 日本マイコプラズマ学会雑誌 35 Pages: 73-74
  • NAID: 10026896315
• [Journal Article] A triacylated lipoprotein from Mycoplasma genitalium activates NF-kappaB through Toll-like receptor 1 (TLR1) and TLR2. (2008)
  • Author(s): Shimizu T, Kida Y, Kuwano K
  • Journal Title: Infect Immun. 76 Pages: 3672-3678
  • Peer Reviewed
• [Journal Article] Mycoplasma pneumoniae-derived lipopeptides induce acute inflammatory responses in the lungs of mice (2008)
  • Author(s): Shimizu. T, Kida. Y, Kuwano. K
  • Journal Title: Infect Immun 76 Pages: 270-277
  • Peer Reviewed
• [Journal Article] Ureaplasma urealyticum lipoproteins, including MB antigen, activate NF-κB through TLR1, TLR2, and TLR6 (2008)
  • Author(s): Shimizu. T, Kida. Y, Kuwano. K
  • Journal Title: Microbiology 154 Pages: 1318-1325
  • Peer Reviewed
• [Journal Article] A novel secreted protease from Pseudomonas aeruginosa activates NF-kappaB through protease-activated receptors. (2008)
  • Author(s): Kida Y, Higashimoto Y, Inoue H, Shimizu T, Kuwano K.
  • Journal Title: Cell Microbiol. 10 Pages: 1491-1504
  • Peer Reviewed
• [Journal Article] A triacylated lipoprotein from Mycoplasma genitalium activates NF-kappaB through Toll-like receptor 1 (TLR1) and TLR2. (2008)
  • Author(s): Shimizu. T, Kida. Y, Kuwano. K
  • Journal Title: Infect Immun 76 Pages: 3672-3678
  • Peer Reviewed
• [Presentation] Mycoplasma pneumoniaeの接着に依存的な炎症誘導 (2010)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Organizer: 第37回日本マイコプラズマ学会
  • Place of Presentation: 東京 国立感染症研究所
  • Year and Date: 2010-06-10
• [Presentation] Mycoplasma pneumoniaeの接着に依存的な炎症誘導 (2010)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Organizer: 日本マイコプラズマ学会第37回学術集会
  • Place of Presentation: 国立感染症研究所
  • Year and Date: 2010-06-10
• [Presentation] マイコプラズマ感染におけるCRAMPの意義の検討 (2010)
  • Author(s): 桑野剛一、清水隆、木田豊
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] Mycoplasma pneumoniaeリポプロテインを標的抗原としたELISAの構築 (2009)
  • Author(s): 桑野剛一、清水隆
  • Organizer: 第36回日本マイコプラズマ学会
  • Place of Presentation: 北海道大学(百年記念館)
  • Year and Date: 2009-06-04
• [Presentation] Mycoplasma pneumoniae リポプロテインを標的抗原としたELISAの構築 (2009)
  • Author(s): 桑野剛一、清水隆
  • Organizer: 日本マイコプラズマ学会第36回学術集会
  • Place of Presentation: 北海道大学 百年記念館
  • Year and Date: 2009-06-04
• [Presentation] Mycoplasma genitalium由来リポタンパク質によるToll-like receptorシグナルの活性化 (2009)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-03-13
• [Presentation] Ureaplasma parvum由来リポタンパク質によるToll-like receptorシグナルの活性化 (2008)
  • Author(s): 清水隆、木田豊、桑野剛一
  • Organizer: 第35回日本マイコプラズマ学会
  • Place of Presentation: 東京大学(弥生講堂)
• [Presentation] Ureaplasma parvum由来リポタンパク質によるToll-like receptorシグナルの活性化 (2008)
  • Author(s): 清水隆, 木田豊, 桑野剛一
  • Organizer: 日本マイコプラズマ学会
  • Place of Presentation: 東京大学, 弥生講堂
• [Remarks] ホームページ等
  • URL: http://www.med.kurume-u.ac.jp/med/micro/
• [Remarks]
  • URL: http://www.med.kurume-u.ac.jp/med/micro/index.html
• [Patent(Industrial Property Rights)] マイコプラズマ感染症用ワクチン組成物 (2009)
  • Inventor(s): 桑野剛一、清水隆
  • Industrial Property Rights Holder: 学校法人久留米大学
  • Filing Date: 2009-04-21
  • Overseas
• [Patent(Industrial Property Rights)] マイコプラズマ感染症用ワクチン組成物 (2008)
  • Inventor(s): 桑野剛一、清水隆
  • Industrial Property Rights Holder: 学校法人久留米大学
  • Filing Date: 2008-05-23
• [Patent(Industrial Property Rights)] マイコプラズマ感染症用ワクチン組成物 (2008)
  • Inventor(s): 桑野剛一, 清水隆
  • Industrial Property Rights Holder: 学校法人久留米大学
  • Filing Date: 2008-05-23