Analysis of urine acidification mechanism through calcium-sensing receptor

• Project/Area Number: 20590942
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Tohoku University
• Principal Investigator: MORIMOTO Tetsuji 東北大学, 病院, 助教 (10344657)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: カルシウム感知受容体 / 腎髄質部太いヘンレの上行脚 / 微小単離尿細管灌流法 / 微小単離細管灌流法 / 細胞内アルカリ化 / 腎髄質部の太いヘンレの上行脚 / H^+-K^+-ATPase / カルシウム感知受容体(CaR) / 髄質部太いヘンレの上行脚 / 尿中落下尿細管細胞培養系

Research Abstract

We examined whether Calcium-sensing receptor (CaR)activationcaused acid secretion in the medullary thick ascending limb of mouse kidney using i^nv^itro microperfusion. We verified that Neomycin (which activates CaR)induced intracellular alkalinization in HCO^-_3/C_O2-buffer. Besides, we succeeded to establish that this regulatory mechanism is luminal K^+-dependent but luminal Na^+ independent for the first time.