| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
5-Aza-2'-deoxycytidine (5-Aza) effecectively induced WT-1 in human embryonic stem cells, KhES-1, 2 and 3. Next we transplanted 5-Aza treated KhES-1, 2 and 3 into the kidney capsule of SCID mice. After 6-24 weeks, we found generation of kidney tubular cells, but not endothelial cells and podocytes. Our results demonstrated that 5-Aza promotes differentiation of human ES cells to kidney cells, especially tubular cells.
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