Proteomic analysis of transgenic rat specifically expressing ADAMTS-8 in podocyte

• Project/Area Number: 20590951
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Niigata University
• Principal Investigator: GOTO Shin Niigata University, 医歯学系, 助教 (00463969)
• Co-Investigator(Kenkyū-buntansha): NARITA Ichiei 新潟大学, 医歯学系, 教授 (20272817) ; YAMAMOTO Tadashi 新潟大学, 医歯学系, 教授 (30092737) ; IGUCHI Seitaro 新潟大学, 大学院・医歯学総合研究科, 特任教授 (20420309)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ADAMTS-8 / transgenic rat / proteome / nephrotic syndrome / phage display / proteinuria / immunoprecipitation

Research Abstract

Nephrotic syndrome present massive proteinuria, however, underling mechanisms remain elusive. We focused an interaction between the cell-cell contact which is involved with urine production, and proteinase produced in these cells (ADAMTS-8). Rat with forced expression of ADAMTS-8 gradually showed proteinuria. Using these rat model, we revealed molecules associated with development of proteinuria.

Research Products

• [Journal Article] Membranoproliferative pattern of glomerular injury associated with complement component 9 deficiency due to Arg95Stop mutation. (2011)
  • Author(s): Miura T, Goto S, Iguchi S, Shimada H, Ueno M, Nishi S, Narita I.
  • Journal Title: Clin Exp Nephrol 15 Pages: 86-91
  • NAID: 10029004557
  • Peer Reviewed
• [Journal Article] Expression of SM22alpha (Transgelin) in Glomerular and Interstitial Renal Injury. (2011)
  • Author(s): Inomata S, Sakatsume M, Sakamaki Y, Wang X, Goto S, Yamamoto T, Gejyo F, Narita I.
  • Journal Title: Nephron Exp Nephrol 117
  • Peer Reviewed
• [Journal Article] Injured kidney cells express SM22α (transgelin) : Unique features distinct from α-smooth muscle actin (αSMA) (2011)
  • Author(s): Sakamaki Y, Sakatsume M, Wang X, Inomata S, Yamamoto T, Gejyo F, Narita I.
  • Journal Title: Nephrology. 16 Pages: 211-218
  • Peer Reviewed
• [Journal Article] Novel expression of claudin-5 in glomerular podocytes. (2011)
  • Author(s): Koda R, Zhao L, Yaoita E, Yoshida Y, Tsukita S, Tamura A, Nameta M, Zhang Y, Fujinaka H, Magdeldin S, Xu B, Narita I, Yamamoto T.
  • Journal Title: Cell Tissue Res. 343 Pages: 637-648
• [Journal Article] Membranoproliferative pattern of glomerular injury associated with complement component 9 deficiency due to Arg95Stop mutation. (2011)
  • Author(s): Miura T, Goto S, et al.
  • Journal Title: Clinical and Experimental Nephrology Volume: 15 Pages: 86-91
  • NAID: 10029004557
  • Peer Reviewed
• [Journal Article] Proteomics database in chronic kidney disease. (2010)
  • Author(s): Yamamoto T.
  • Journal Title: Adv Chronic Kidney Dis. 17 Pages: 487-492
  • Peer Reviewed
• [Journal Article] Clinicopathological characteristics of patients with IgG4-related tubulointerstitial nephritis. (2010)
  • Author(s): Saeki T, Nishi S, Imai N, Ito T, Yamazaki H, Kawano M, Yamamoto M, Takahashi H, Matsui S, Nakada S, Origuchi T, Hirabayashi A, Homma N, Tsubata Y, Takata T, Wada Y, Saito A, Fukase S, Ishioka K, Miyazaki K, Masaki Y, Umehara H, Sugai S, Narita I.
  • Journal Title: Kidney Int 78 Pages: 1016-1023
  • Peer Reviewed
• [Journal Article] Uremic pruritus in chronic hemodialysis patients. (2008)
  • Author(s): Narita I, Iguchi S, Omori K, Gejyo F.
  • Journal Title: J Nephrol 21 Pages: 161-165
  • Peer Reviewed
• [Journal Article] Pathogenetic significance of aberrant glycosylation of IgA1 in IgA nephropathy. (2008)
  • Author(s): Narita I, Gejyo F.
  • Journal Title: Clin Exp Nephrol 12 Pages: 332-338
  • Peer Reviewed
• [Journal Article] Pathogenetic significance of aberrant glycosylation of IgAl in IgA nephropathy. (2008)
  • Author(s): Narita I, Gejyo F.
  • Journal Title: Clinical and Experimental Nephrology 12 Pages: 332-338
  • Peer Reviewed
• [Journal Article] Uremic pruritis in chronic hemodialysis patients. (2008)
  • Author(s): Narita I, Iguchi S, et al.
  • Journal Title: Journal of Nephrology 21 Pages: 161-165
  • Peer Reviewed
• [Presentation] Urinary angiotensinogen as a marker for histopathological severity inpatients with IgA nephropathy. (2010)
  • Author(s): Iguchi A, Goto S, et al.
  • Organizer: International Society of Nephrology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2010-04-15
• [Presentation] Urinary angiotensinogen as a marker for histopathological severity in patients with IgA nephropathy. (2009)
  • Author(s): Iguchi A, Goto S, et al.
  • Organizer: American Society of Nephrology
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2009-10-29
• [Presentation] Urinary angiotensinogen as a marker for histopathological severity in patients with IgA nephropathy. (2009)
  • Author(s): Iguchi A, Goto S, et al.
  • Organizer: International Society of Nephrology
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-04-15
• [Presentation] Aberrant glycosylation of IgA1 is heritable in familial IgA nephropathy. (2008)
  • Author(s): Wada M, Goto S, et al.
  • Organizer: American Scociety of Nephrology
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2008-11-08
• [Presentation] Aberrant glycosylation of IgAl is heritable in familial IgA nephropathy. (2008)
  • Author(s): Wada M, Goto S, et al.
  • Organizer: American Society of Nephrology
  • Place of Presentation: Philadelphia, PA, USA
  • Year and Date: 2008-11-08