Identifications for molecular mechanisms of essential hypertension in terms of urinary tubular ion transporter and human NEDD4L.

• Project/Area Number: 20590978
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Yokohama City University
• Principal Investigator: ISHIGAMI Tomoaki Yokohama City University, 医学部, 准教授 (50264651)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 尿細管 / ナトリウム輸送 / Nedd4L / 本態性高血圧症 / 心腎連関 / 食塩感受性 / イオントランスポーター / ナトリウムチャンネル / 尿細管レニンアンジオテンシン系 / 上皮性ナトリウムチャンネル / 腎臓尿細管 / ユビキチン化 / NPC2 / イオンチャンネル / ユビキチン

Research Abstract

Nedd4L has been paid attention for its unique ubiquitinating properties that does an important working to cuticle sodium channel (ENaC) in the tubular epithelial cell and the post-transcriptional modification so far. Clarification and study results of the urinary tubular sodium re-absorption mechanism, and the role of Nedd4L in cardio-renal relationship is examined and the research announcement is done.

Research Products

• [Journal Article] Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice. (2010)
  • Author(s): Wakui H, Tamura K, Matsuda M, Bai Y, Dejima T, Shigenaga A, Masuda S, Azuma K, Maeda A, Hirose T, Ishigami T, Toya Y, Yabana M, Minamisawa S, Umemura S.
  • Journal Title: Am J Physiol Renal Physiol. 299(5)
• [Journal Article] Expression of angiotensin II type 1 receptor-interacting molecule in normal human kidney and IgA nephropathy. (2010)
  • Author(s): Masuda S, Tamura K, Wakui H, Maeda A, Dejima T, Hirose T, Toyoda M, Azuma K, Ohsawa M, Kanaoka T, Yanagi M, Yoshida S, Mitsuhashi H, Matsuda M, Ishigami T, Toya Y, Suzuki D, Nagashima Y, Umemura S.
  • Journal Title: Am J Physiol Renal Physiol. 299(4)
• [Journal Article] Cardiac-specific activation of angiotensin II type 1 receptor-associated protein completely suppresses cardiac hypertrophy in chronic angiotensin II-infused mice. (2010)
  • Author(s): Wakui H, Tamura K, Tanaka Y, Matsuda M, Bai Y, Dejima T, Masuda S, Shigenaga A, Maeda A, Mogi M, Ichihara N, Kobayashi Y, Hirawa N, Ishigami T, Toya Y, Yabana M, Horiuchi M, Minamisawa S, Umemura S.
  • Journal Title: Hypertension 55(5) Pages: 1157-1164
• [Journal Article] Human Nedd4L rs4149601 G allele generates evolutionary new isoform I with C2 domain. (2010)
  • Author(s): Ishigami T, Araki N, Umemura S.
  • Journal Title: Hypertension 55(2)
• [Journal Article] Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice. (2010)
  • Author(s): Wakui H, Ishigami T, et al.
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(5)
  • Peer Reviewed
• [Journal Article] Expression of angiotensin II type 1 receptor-interacting molecule in normal human kidney and IgA nephropathy. (2010)
  • Author(s): Masuda S, Ishigami T, et al.
  • Journal Title: Am J Physiol Renal Physiol Volume: 299(4)
  • Peer Reviewed
• [Journal Article] Cardiac-specific activation of angiotensin II type 1 receptor-associated protein completely suppresses cardiachypertrophy in chronic angiotensin II-infused mice. (2010)
  • Author(s): Wakui H, Ishigami T, et al.
  • Journal Title: Hypertension. Volume: 55(5) Pages: 1157-64
  • Peer Reviewed
• [Journal Article] Blood pressure variability as well as blood pressure level is important for left ventricular hypertrophy and brachial-ankle pulse wave velocity in hypertensives. (2009)
  • Author(s): Ozawa M, Tamura K, Okano Y, Matsushita K, Ikeya Y, Masuda S, Wakui H, Dejima T, Shigenaga A, Azuma K, Ishigami T, Toya Y, Ishikawa T, Umemura S.
  • Journal Title: Clin Exp Hypertens. 31(8) Pages: 669-679
• [Journal Article] Effect of losartan on ambulatory short-term blood pressure variability and cardiovascular remodeling in hypertensive patients on hemodialysis. (2009)
  • Author(s): Mitsuhashi H, Tamura K, Yamauchi J, Ozawa M, Yanagi M, Dejima T, Wakui H, Masuda S, Azuma K, Kanaoka T, Ohsawa M, Maeda A, Tsurumi-Ikeya Y, Okano Y, Ishigami T, Toya Y, Tokita Y, Ohnishi T, Umemura S.
  • Journal Title: Atherosclerosis 207(1) Pages: 186-190
• [Journal Article] Identification of NPC2 protein as interaction molecule with C2 domain of human Nedd4L. (2009)
  • Author(s): Araki N, Ishigami T, Ushio H, Minegishi S, Umemura M, Miyagi Y, Aoki I, Morinaga H, Tamura K, Toya Y, Uchino K, Umemura S.
  • Journal Title: Biochem Biophys Res Commun. 388(2) Pages: 290-296
• [Journal Article] Utility and feasibility of a new programmable home blood pressure monitoring device for the assessment of nighttime blood pressure. (2009)
  • Author(s): Ushio H, Ishigami T, Araki N, Minegishi S, Tamura K, Okano Y, Uchino K, Tochikubo O, Umemura S.
  • Journal Title: ClinExp Nephrol. 13(5) Pages: 480-485
  • NAID: 10027226625
• [Journal Article] Left ventricular geometry, risk factors, and outcomes of hospitalized patients with diastolic heart failure in Japan. (2009)
  • Author(s): Uchino K, Ishigami T, Ohshige K, Sugano T, Ishikawa T, Kimura K, Umemura S.
  • Journal Title: J Cardiol. 54(1) Pages: 101-107
  • NAID: 10025184500
• [Journal Article] Identification of an increased short-term blood pressure variability on ambulatory blood pressure monitoring as a coronary risk factor in diabetic hypertensives. (2009)
  • Author(s): Ozawa M, Tamura K, Okano Y, Matsushita K, Yanagi M, Tsurumi-Ikeya Y, Oshikawa J, Hashimoto T, Masuda S, Wakui H, Shigenaga A, Azuma K, Ishigami T, Toya Y, Ishikawa T, Umemura S.
  • Journal Title: Clin Exp Hypertens. 31(3) Pages: 259-270
• [Journal Article] Effects of angiotensin II type 1 receptor blocker on blood pressure variability and cardiovascular remodeling in hypertensive patients on chronic peritoneal dialysis. (2009)
  • Author(s): Shigenaga A, Tamura K, Dejima T, Ozawa M, Wakui H, Masuda S, Azuma K, Tsurumi-Ikeya Y, Mitsuhashi H, Okano Y, Kokuho T, Sugano T, Ishigami T, Toya Y, Uchino K, Tokita Y, Umemura S.
  • Journal Title: Nephron Clin Pract. 112(1)
• [Journal Article] Combining chronic kidney disease with201thallium/123iodine beta methyliodophenyl pentadecanoic acid dual myocardial single-photon emission computed tomography findings is useful for the evaluation of cardiac event risk. (2009)
  • Author(s): Takamura T, Takahashi N, Ishigami T, Sugano T, Ishikawa T, Uchino K, Kimura K, Inoue T, Umemura S.
  • Journal Title: Nucl Med Commun. 30(1) Pages: 54-61
• [Journal Article] Human Nedd4L rs4149601 G allele generates evolutionary new isoforml with C2 domain (2009)
  • Author(s): Ishigami T, Araki N, Umemura
  • Journal Title: Hypertension 55(2)
  • Peer Reviewed
• [Journal Article] Identification of NPC2 protein as interaction molecule with C2 domain of human Nedd4L (2009)
  • Author(s): Araki N, Ishigami T, et al.
  • Journal Title: Biochem Biophys Res Commun 31(8) Pages: 669-79
  • Peer Reviewed
• [Journal Article] Utility and feasibility of a new programmable home blood pressure monitoring device for the assessment of nighttime blood pressure (2009)
  • Author(s): Ushio H, Ishigami T, et al.
  • Journal Title: Clinical Experimental Nephrology 13(5) Pages: 480-485
  • NAID: 10027226625
  • Peer Reviewed
• [Journal Article] Urinary oxidative stress markers closely reflect the efficacy of candesartan treatment for diabetic nephropathy. (2009)
  • Author(s): Yoshida, S, Ishigami, T, et al.
  • Journal Title: Nephron Exp Nephrolo 111
  • Peer Reviewed
• [Journal Article] Combining chronic kidney disease with 201thallium/123iodine beta methlyiodophenly pentadecanoic acid dual myocardial single-photon emission computed tomography findings is useful for the evaluation of cardiac event risk. (2009)
  • Author(s): Takamura, T, Ishigami, T, et al.
  • Journal Title: Nucl Med Commun 30 Pages: 54-61
  • Peer Reviewed
• [Journal Article] Identification of an increased short-term blood pressure variability on ambulatory blood pressure monitoring as a coronary risk factor in diabetic hypertensives. (2009)
  • Author(s): Ozawa, M, Ishigami, T, et. al.
  • Journal Title: Clin Exp Hypertens 31 Pages: 259-70
  • Peer Reviewed
• [Presentation] ANGIOTENSIN II INDUCED SCN5A-NEDD4L TRANSCRIPTIONAL ACTIVATION WITH MINERAL CORTICOID RECEPTOR TRANSACTIVATION IN CARDIOMYOCYTES (2010)
  • Author(s): Minegishi S, Ishigami T, et al.
  • Organizer: 第20回ヨーロッパ高血圧学会
  • Place of Presentation: Norway, Oslo
  • Year and Date: 2010-06-20
• [Presentation] ALDOSTERONE INDEPENDENT ACTIVATION OF MINERALCORTICOID RECEPTOR-SGK1 SYSTEMS IN URINARY EPITHELIUM FOR THE MODEL OF SODIUM SENSITIVE HYPERTENSION (2010)
  • Author(s): Ushio H, Ishigami T, et al.
  • Organizer: 第20回ヨーロッパ高血圧学会
  • Place of Presentation: Norway, Oslo
  • Year and Date: 2010-06-19
• [Presentation] ALDOSTERONE INDEPENDENT ACTIVATION OF MINERALCORTIC OID RECEPTOR-SGK1 SYSTEMS IN URINARY EPITHELIUM FOR THE MODEL OF SODIUM SENSITIVE HYPERTENSION (2010)
  • Author(s): Ushio H, Ishigami T, et al.
  • Organizer: 第20回ヨーロッパ高血圧学会
  • Place of Presentation: Norway, Oslo
  • Year and Date: 2010-06-19
• [Presentation] A New Programmable Home Blood Pressure Monitoring Device For The Assessment of Night Time Blood Pressure of Total Of 40 Normotensive Subjects (2010)
  • Author(s): 石上友章, et al.
  • Organizer: 第20回ヨーロッパ高血圧学会
  • Place of Presentation: Norway, Oslo
  • Year and Date: 2010-06-18
• [Presentation] A New Programmable Home Blood Pressure Monitoring Device For The Assessment of Night Time Blood Pressure of Total Of 40 Nromotensive Subjects (2010)
  • Author(s): Ishigami T, et al.
  • Organizer: 第20回ヨーロッパ高血圧学会
  • Place of Presentation: Norway, Oslo
  • Year and Date: 2010-06-18
• [Presentation] 本態性高血圧症の候補遺伝子としてのNedd4Lに関する検討 (2009)
  • Author(s): 石上友章
  • Organizer: 日本腎臓学会学術総会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-06-05
• [Presentation] Identification of binding proteins for human Nedd4L C2 domain. (2008)
  • Author(s): Araki, N, Ishigami, T, et al.
  • Organizer: 22^<nd> International Society of Hypertension, Berlin, Germany
  • Place of Presentation: Berlin, Germany
  • Year and Date: 2008-06-15
• [Book] 選択的アルドステロンブロッカーのすべて (2009)
  • Author(s): 石上友章, ほか
  • Total Pages: 239
  • Publisher: 先端医学社
• [Book] 高血圧専門医ガイドブック (2009)
  • Author(s): 日本高血圧学会(編)共著
  • Total Pages: 226
  • Publisher: 診断と治療社
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