Mechanism elucidation of calcification with phosphate-induced and endothelial injury in rat aortic tissue culture

• Project/Area Number: 20590981
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Kidney internal medicine
• Research Institution: Wakayama Medical University
• Principal Investigator: HATAMURA Ikuji Wakayama Medical University, 保健医療学部, 教授 (80336883)
• Co-Investigator(Kenkyū-buntansha): SHIIZAKI Kazuhiro 自治医科大学, 医学部, 助教 (10423930)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 血管石灰化 / 高リン / 血管内膜障害 / 血管培養 / 高リン負荷 / 内膜障害 / メンケベル型血管石灰化 / PTA

Research Abstract

The cardiovascular calcification is one of the factors to cause the cardiovascular complication that is the first place of the cause of death of the hemodialysis patients. Therefore to control the vascular calcification prevents the complication of the patient and becomes lower the death rate of the patients. In this study, it was confirmed that vascular calcification was more strongly induced in the high serum phosphorus that most of hemodialysis patients contracted a disease with endothelial injury. It was suggested that it was necessary for the calcification prevention after endarterectomy to improve hyperphosphatemia in patients.

Research Products

• [Journal Article] Development of a technique for intro-duction of an expressed complementary de-oxyribonucleic acid into parathyroid ce-lls by direct injection. (2010)
  • Author(s): Shiizaki K, Hatamura I, Fukagawa M, Kusano E, et al.
  • Journal Title: Endocrinology. 151(8) Pages: 4031-8
• [Journal Article] Reduced expression of perlecan in the aorta of secondary hyperparathyroidism model rats with medial calcification. (2010)
  • Author(s): Shibata M, Shigematsu T, Hatamura I, Saji F, Mune S, Kunimoto K, Hanba Y, Shiizaki K, et al.
  • Journal Title: Ren Fail. 32(2) Pages: 214-23
• [Journal Article] Development of a technique for introduction of an expressed complementary deoxyribonucleic acid into parathyroid cells by direct injection. (2010)
  • Author(s): Shiizaki K, Hatamura I, 等
  • Journal Title: Endocrinology. Volume: 151(8) Pages: 4031-4038
  • Peer Reviewed
• [Journal Article] Reduced expression of perlecan in the aorta of secondary hyperparathyroidism model rats with medial calcification. (2010)
  • Author(s): Shibata M, Hatamura I, 等
  • Journal Title: Ren Fail. Volume: 32(2) Pages: 214-223
  • Peer Reviewed
• [Journal Article] Trps1 functions downstream of Bmp7 in kidney development. (2009)
  • Author(s): Gai Z, Zhou G, Itoh S, Hatamura I, Muragaki Y, et al.
  • Journal Title: 20(11) Pages: 2403-11
• [Journal Article] Mechanism of phosphate-induced calc-ifyication in rat aortic tissue culture : possible involvement of Pit-1 and ap-optosis. (2009)
  • Author(s): Mune S, Hatamura I, Saji, et al.
  • Journal Title: Clin Exp Nephrol. 111(4) Pages: 59-66
• [Journal Article] Improvement of impaired calcium and sk-eletal homeostasis in vitamin D receptor knockout mice by a high dose of calcitriol and maxacalcitol. (2009)
  • Author(s): Shiizaki K, Hatamura I, Akizawa T, Kusano E., et al.
  • Journal Title: Bone. 45(5) Pages: 964-71
• [Journal Article] Regulation of fibroblast growth factor 23 production in bone in uremic rats. (2009)
  • Author(s): Saji F, Shiizaki K, Hatamura I, 等
  • Journal Title: Nephron Physiol. 111(4) Pages: 59-66
  • Peer Reviewed
• [Journal Article] Tolvaptan, a selective oral vasopressin V2 receptor antagonist, ameliorates podocyte injury in puromycin aminonucleoside nephrotic rats. (2009)
  • Author(s): Okada T, Hatamura I, 等
  • Journal Title: Clin Exp Nephrol. 13(5) Pages: 438-46
  • NAID: 10027226471
  • Peer Reviewed
• [Journal Article] Improvement of impaired calcium and skeletal homeostasis in vitamin Dreceptor knockout mice by a high dose of calcitriol and maxacalcitol. (2009)
  • Author(s): Shiizaki K, Hatamura I, 等
  • Journal Title: Bone. 45(5) Pages: 964-71
  • Peer Reviewed
• [Journal Article] Mechanism of phosphate-induced calcification in rat aortic tissue culture : possible involvement of Pit-1 and apoptosis. (2009)
  • Author(s): Mune S, Hatamura I, 等
  • Journal Title: Clin Exp Nephrol. 13(6) Pages: 571-7
  • NAID: 10027227436
  • Peer Reviewed
• [Journal Article] Trps1 functions downstream of Bmp7 in kidney development. (2009)
  • Author(s): Gai Z, Hatamura I, 等
  • Journal Title: J Am Soc Nephrol. 20(11) Pages: 2403-11
  • Peer Reviewed
• [Journal Article] Direct injection of calcitriol or its analog abnormal gene expression in the hy-perplastic parathyroid gland in uremia. (2008)
  • Author(s): Shiizaki K, Hatamura I, Akizawa T, et al.
  • Journal Title: Am J Nephrol 28 Pages: 59-66
• [Journal Article] Direct injection of calcitriol or its analog improes abnormal gene expression in the hyperplastic parathyroid gland in uremia (2008)
  • Author(s): Shiizaki K, Hatamura I 等
  • Journal Title: Am J Nephrol. 28 Pages: 59-66
  • Peer Reviewed
• [Journal Article] Highly concentrated calcitriol and its analogues induce apoptosis of parathyroid cells and regression of the hyperplastic gland-study in rats. (2008)
  • Author(s): Shiizaki K, Hatamura I 等
  • Journal Title: Nephrol Dial Transplant. 23 Pages: 1529-1536
  • Peer Reviewed
• [Journal Article] Molecular and morphological approach of uremia-induced hyperplastic parathyroid gland followingdirect maxacalcitol injection. (2008)
  • Author(s): Shiizaki K, Hatamura I 等
  • Journal Title: Med Mol Morphol. 41 Pages: 76-82
  • Peer Reviewed
• [Presentation] Functional induction of target genes in parathyroid cell by direct injection technique. (2008)
  • Author(s): Shiizaki K, Hatamura I, et al.
  • Organizer: 41th Annual Meeting of American Society of Nephrology.
  • Place of Presentation: Philadelphia.
• [Presentation] Functional induction of target genes in parathyroid cell by direct injection technique. (2008)
  • Author(s): Shiizaki K, Hatamura I 等
  • Organizer: 41th Annual Meeting of American Society of Nephrology.
  • Place of Presentation: Philadelphia
• [Remarks] ホームページ等