Elucidation of mechanisms for the decrease in kallikrein secretion in isolated renal connecting tubular cells and examination of salt sensitivity on polymorphisms
| Project/Area Number |
20590983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
FUJITA Tomoe Kitasato University, 医学部, 講師 (20296510)
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| Co-Investigator(Kenkyū-buntansha) |
MAJIMA Masataka 北里大学, 医学部, 教授 (70181641)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | マイクロダイセクション / 腎接合尿細管 / 腎カリクレイン / カルシウムイメージング / SV40ラージT抗原遺伝子導入ラット / 遺伝子多型 / 自然発症高血圧ラット / SV40ラージT抗原ラット / 接合尿細管継代培養 / カリクレイン分泌 / 食塩感受性高血圧 / 賢接合尿細管 / カルシウム動態 / 不死化遺伝子導入ラット / 多型 |
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| Research Abstract |
(1) We have established a calcium imaging model on primary cultured rat renal connecting tubules. Vasopressin increases intracellular calcium via a V1a receptor. (2) We have tried to obtain monoclonal cells of the kallikrein secreting cell by maximum diluting cultured cells derived from the renal initial collecting tubules isolated from SV40 large T antigen transgenic rats. Most of the cultured cells were intercalated cells and kallikrein secreting cells were few. (3) Six subjects among the 77 healthy Japanese volunteers have the polymorphism in the renal kallikrein promoter. Urine kallikrein activity was lower and sodium excretion was higher after water loading in the subjects with the promoter polymorphism than the subjects without the polymorphism.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Safety, tolerability and pharmacokinetics of TAS-108, a novel anti-oestrogen, in healthy post-menopausal Japanese women : a phase I single oral dose study.2009
Author(s)
Kumagai Y, Fujita T, Ozaki M, Yokota S, Maeda M, Shida M, Otani Y, Yamaya H, Tsuruta H
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Journal Title
Basic Clin Pharmacol Toxicol 104/5
Pages: 352-359
Related Report
Peer Reviewed
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[Journal Article] Safety, tolerability and pharmacokinetics of TAS-108, a novel anti-oestrogen, in healthy post-menopausal Japanese women : a phase I single oral dose study.2009
Author(s)
Kumagai Y, Fujita T, Ozaki M, Yokota S, Maeda M, Shida M, Otani Y, Yamaya H, Tsuruta H.
-
Journal Title
Basic Clin Pharmacol Toxicol 104/5
Pages: 352-359
Related Report
Peer Reviewed
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