Analysis of the inhibiting factor in prion infectivity

• Project/Area Number: 20591001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurology
• Research Institution: Nagasaki University
• Principal Investigator: KATSUYA Satoh Nagasaki University, 医歯薬学総合研究科, 講師 (70398147)
• Co-Investigator(Kenkyū-buntansha): ICHIRO Miyoshi 名古屋大学, 医学研究科, 教授 (10183972)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: プリオン病 / 感染抑制因子 / オステオポンチン

Research Abstract

The normal prion protein is essential to the deposition of the abnormal prion protein. The normal prion protein is present with kidney or the testis with the prion infection mouse, but the abnormality prion protein does not deposit it in the kidney or testis. In other words, unlike the other organs, we show that the normal prion protein is not the place of the conformation of the abnormal prion protein. The possibility that the normal prion protein inhibited the conformation change of the abnormal prion protein was thought about by some kind of mechanisms.
We analyzed and compared the difference of the gene expression in the mRNA with the kidney with the prion infection mouse with the normal mice in prion infection mouse kidney by cDNA substration assay.In this assay, awe think that one over-expression or low-expression in the kidney of the prion infection mouse is the candidate of so-called prion suppressive gene.
We were able to find six candidate genes by this procedure. A gene expression could identify low-expression gene with the kidney and over-expression gene with the brain. We found the gene which one genes were the most suitable to think about in the six candidate genes most and identified genes. The making of the genetic knockout mouse is successful and we started to perform the infected-prion experiment in the knockout mouses.

Research Products

• [Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. (2011)
  • Author(s): Atarashi R, Satoh K, Sano K, et al.
  • Journal Title: Nature medicine. 17 Pages: 175-178
• [Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion. (2011)
  • Author(s): Atarashi R, Satoh K, Sano K, et.al.
  • Journal Title: Nature medicine Volume: 17 Pages: 175-178
  • Peer Reviewed
• [Journal Article] Establishment of a standard 14-3-3 protein assay of cerebrospinal fluid as a diagnostic tool for Creutzfeldt-Jakob disease. (2010)
  • Author(s): Satoh K, Tobiume M, Matsui Y, Mutsukura K, Nishida N, Shiga Y, Eguhchi K, Shirabe S, Sata T
  • Journal Title: Lab Invest. 90(11) Pages: 1637-1644
• [Journal Article] Anti-cyclic citrullinated peptide antibody (anti-CCP antibody) is present in the sera of patients with dementia of Alzheimer's type in Asian. (2010)
  • Author(s): Satoh K, Kawakami A, Shirabe S, Tamai M, Sato A, Tsujihata M, Nagasato K, Eguch K
  • Journal Title: Acta Neurol Scand. 121(5) Pages: 338-341
• [Journal Article] Development of an Ultra-Rapid Diagnostic Method Based on Heart-Type Fatty Acid Binding Protein Levels in the CSF of CJD Patients. (2010)
  • Author(s): Matsui Y, Satoh K, Mutsukura K, Watanabe T, Nishida N, Matsuda H, Sugino M, Shirabe S, Eguchi K, Kataoka Y
  • Journal Title: Cell Mol Neurobiol. 30(7) Pages: 991-999
• [Journal Article] The power Doppler ultrasonography score from 24 synovial sites or 6 simplified synovial sites (2010)
  • Author(s): Kawashiri SY, Kawakami A, Iwamoto N, Fujikawa K, Satoh K, Tamai M, Nakamura H, Okada A, Koga T, Yamasaki S, Ida H, Origuchi T, Eguchi K
  • Journal Title: including the metacarpophalangeal joints, reflects the clinical disease activity and level of serum biomarkers in patients with rheumatoid arthritis Rheumatology (Oxford). Epub ahead of print
• [Journal Article] Clinical, serologic and magnetic resonance imaging of 3 cases of inflammatory myopathy with abundant macrophages in the Japanese population. (2010)
  • Author(s): Hara S, Henmi T, Kawakami A, Fujikawa K, Mukae H, Ishimatsu Y, Sakamoto N, Kakugawa T, Kaji K, Fujimoto M, Kuwana M, Tsukada T, Satoh K, Motomura M, Tamai M, Nakamura H, Ida H, Hayashi T, Origuchi T, Eguchi K, Kohno S
  • Journal Title: Rheumatol Int. Epub ahead of print
• [Journal Article] Rheumatoid vasculitis of crural muscles confirmed by muscle biopsy in the absence of inflammatory myopathy : histologic and MRI study. (2010)
  • Author(s): Nakamura H, Okada A, Kawakami A, Yamasaki S, Ida H, Masuda T, Fukuda T, Satoh K, Yoshimura T, Nakashima M, Hayashi T, Eguchi K
  • Journal Title: Rheumatol Int. 30(10) Pages: 1381-1383
• [Journal Article] Early detection of sporadic CJD by diffusion-weighted MRI before the onset of symptoms. (2010)
  • Author(s): Satoh K, Nakaoke R, Nishiura Y, Tsujino A, Motomura M, Yoshimura T, Sasaki K, Shigematsu K, Shirabe S, Eguchi K
  • Journal Title: J Neurol Neurosurg Psychiatry. Epub ahead of print
• [Journal Article] Establishment of a standard 14-3-3 protein assay of cerebrospinal fluidas a diagnostic tool for Creutzfeldt-Jakob disease. (2010)
  • Author(s): Satoh K, Tobiume M, Matsui Y, et al.
  • Journal Title: Laboratory investigation ; a journal of technical methods and pathology. Volume: 90 Pages: 1637-1644
  • Peer Reviewed
• [Journal Article] Anti-cyclic citrullinated peptide antibody (anti-CCP antibody) is present in the sera of patients with dementia of Alzheimer's type in Asian (2010)
  • Author(s): Satoh K, Kawakami A, Shirabe S, et al.
  • Journal Title: Acta neurologica Scandinavica Volume: 121 Pages: 338-341
  • Peer Reviewed
• [Journal Article] Development of an ultra-rapid diagnostic method based on heart-type fatty acid binding protein levels in the CSF of CJD patients. (2010)
  • Author(s): Matsui Y, Satoh K, Mutsukura K, et al.
  • Journal Title: Cellular and molecular neurobiology Volume: 30 Pages: 991-999
  • Peer Reviewed
• [Journal Article] Rheumatoid vasculitis of crural muscles confirmed by muscle biopsy in the absence of inflammatory myopathy : histologic and MRI study. (2010)
  • Author(s): Nakamura H, Okada A, Kawakami A, et al.
  • Journal Title: Rheumatology international Volume: 30 Pages: 1381-1383
  • Peer Reviewed
• [Journal Article] Rheumatoid vasculitis of crural muscles confirmed by muscle biopsy in the absence of inflammatory myopathy : histologic and MRI study. (2009)
  • Author(s): Nakamura H, Okada A, Kawakami A, Yamasaki S, Ida H, Masuda T, Fukuda T, Satoh K, Yoshimura T, Nakashima M, Hayashi T, Eguchi K
  • Journal Title: Rheumatology international (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Anti-cyclic citrullinated peptide antibody(anti-CCP antibody)is present in the sera of patients with dementia of Alzheimer's type in Asian. (2009)
  • Author(s): Satoh K, Kawakami A, Shirabe S, Tamai M, Sato A, Tsujihata M, Nagasato K, Eguchi K.
  • Journal Title: Acta neurologica Scandinavica. (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Different expression levels of TNF receptors on the rheumatoid synovial macrophages derived from surgery and a synovectomy as detected by a new flow cytometric analysis. (2009)
  • Author(s): Ida H, Aramaki T, Nakamura H, Fujikawa K, Arima K, Tamai M, Kamachi M, Satoh K, Origuchi T, Kawakami A, Furuichi I, Kawabe Y, Eguchi K
  • Journal Title: Cytotechnology. 60 Pages: 161-164
  • Peer Reviewed
• [Journal Article] Familial Creutzfeldt-Jakob disease with a V180I mutation : comparative analysis with pathological findings and diffusion-weighted images. (2009)
  • Author(s): Mutsukura K, Satoh K, Shirabe S, Tomita I, Fukutome T, Morikawa M, Iseki M, Sasaki K, Shiaga Y, Kitamoto T, Eguchi K.
  • Journal Title: Dementia and geriatric cognitive disorders. 28 Pages: 550-557
  • Peer Reviewed
• [Journal Article] Rheumatoid vasculitis of crural muscles confirmed by muscle biopsy in the absence of inflammatory myopathy : histologic and MRI study. (2009)
  • Author(s): Nakamura H, Okada A, Kawakami A, Yamasaki S, Ida H, Masuda T, Fukuda T, Satoh K, Yoshimura T, Nakashima M, Hayashi T, Eguchi K..
  • Journal Title: Rheumatology international. 2009 (Epub ahead of print)
  • Peer Reviewed
• [Journal Article] Bone marrow stroma cells are susceptible to prion infection. (2008)
  • Author(s): Takakura Y, Yamaguchi N, Nakagaki T, Satoh K, Kira J, Nishida N
  • Journal Title: Biochem Biophys Res Commun. 377(3) Pages: 957-61
  • Peer Reviewed
• [Journal Article] 【透析患者診療のための診断基準・重症度スコア 適切な病態評価のために】臨床所見・徴候からのアプローチ 麻痺 (2008)
  • Author(s): 佐藤克也, 本村政勝
  • Journal Title: 腎と透析 24 Pages: 814-816
• [Presentation] プリオン病患者脳脊髄液中診断マーカーの比較検討 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
  • Organizer: 第51回神経学会総会
  • Place of Presentation: 東京
• [Presentation] CJDにおける血清バイオマーカーに用いた血液脳関門(BBB)についての検討 (2010)
  • Author(s): 六倉和生、佐藤克也、調漸、岸田日帯、黒岩義之、三條伸夫、水澤英洋
  • Organizer: 第51回神経学会総会
  • Place of Presentation: 東京
• [Presentation] Analysis of Biochemical markers and the method of abnormal prion protein in CSF in Human Prion Diseases as Diagnostic markers (2010)
  • Author(s): Satoh K, Atarashi R, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Amelia McGlade, Steven John Collins, Shirabe S, Nishida N
  • Organizer: BIT's 1st Annual World Congress of NeuroTalk-2010.
  • Place of Presentation: シンガポール
• [Presentation] Ultrasensitive human prion detection in cerebrospinal fluids using real-time quaking-induced conversion (2010)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamanaka H, Yamaguchi N, Ishibashi D, Matsubara T,Nakagaki T, Yamada M, Mizusawa H, Kitamoto T, Amelia McGlade, Steven John Collins, Shirabe S, Katamine S, Nishida N
  • Organizer: Asia-Oceania Symposium on Prion Diseases
  • Place of Presentation: 札幌
• [Presentation] Analysis of Biochemical markers and the method of abnormal prion protein in CSF in Human Prion Diseases as Diagnostic markers (2010)
  • Author(s): Satoh K, Atarashi R, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Amelia McGlade, Steven John Collins, Shirabe S, Nishida N
  • Organizer: Asia-Oceania Symposium on Prion Diseases
  • Place of Presentation: 札幌
• [Presentation] Ultrasensitive human prion detection in cerebrospinal fluids by real-time quaking-induced conversion (2010)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamanaka H, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamada M, Mizusawa H, Kitamoto T, Amelia McGlade, Steven John Collins, Shirabe S, Katamine S, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
• [Presentation] Genetic Prion disease in Japan, An analysis based on the Japanese CJD Surveillance,1999-2009 (2010)
  • Author(s): Ohara M, Sanjo N, Hizume M, Sakai K, Nozaki I, Hamaguchi T, Nakamura Y, Kitamoto T, Shiga Y, Satoh K, Satoh T, Shirabe S, Yamada M, Tateishi J, Mizusawa H
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
• [Presentation] Evaluation of a sandwich ELISA for the gamma-isoform of 14-3-3 proteins for laboratory diagnosis of Creutzfeldt-Jakob disease (2010)
  • Author(s): Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Kataoka Y, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
• [Presentation] Brain MRI activity and serum biochemical markers for evaluating blood-brain barrier function in Creutzfeldt-Jakob disease (2010)
  • Author(s): Satoh K, Mutsukura K, Atarashi R, Shirabe S, Matsui Y, Kishida H, Kuroiwa Y, Sanjo N, Mizusawa H, Nishida N
  • Organizer: PRION 2010
  • Place of Presentation: Salzburg, Austria
• [Presentation] プリオン病患者脳脊髄液中診断マーカーの比較検討 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
  • Organizer: 日本神経学会総会
  • Place of Presentation: 東京
• [Presentation] CJDにおける血清バイオマーカーに用いた血液脳関門(BBB)についての検討 (2010)
  • Author(s): 六倉和生、佐藤克也、調漸、岸田日帯、黒岩義之、三條伸夫、水澤英洋
  • Organizer: 日本神経学会総会
  • Place of Presentation: 東京
• [Presentation] Biochemical markersses and abnormal prion protein in CSF of prion diseases (2010)
  • Author(s): Satoh K, Atarashi R, Shirabe S, Nishida N
  • Organizer: BIT's 1st Annual World Congress of NeuroTalk-2010
  • Place of Presentation: シンガポール
• [Presentation] Brain MRI activity and serum biochemical markers for evaluating blood-brain barrier function in Creutzfeldt-Jakob disease (2010)
  • Author(s): Satoh K, Mutsukura K, Atarashi R, Shirabe S, Matsui Y, Kishida H, Kuroiwa Y, Sanjo N, Mizusawa H, Nishida N
  • Organizer: Prion 2010
  • Place of Presentation: オーストリア
• [Presentation] Evaluation of a sandwich ELISA for the gamma-isoform of 14-3-3 proteins for laboratory diagnosis of Creutzfeldt-Jakob disease (2010)
  • Author(s): Matsui Y, Satoh K, Miyazaki T, Shirabe S, Atarashi R, Kataoka Y, Nishida N
  • Organizer: Prion 2010
  • Place of Presentation: オーストリア
• [Presentation] Ultrasensitive human prion detection in cerebrospinal fluids by real-time quaking-induced conversion (2010)
  • Author(s): Atarashi R, Satoh K, Sano K, Fuse T, Yamanaka H, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamada M, Mizusawa H, Kitamoto T, Amelia McGIade, Steven John Collins, Shirabe S, Katamine S, Nishida N
  • Organizer: Prion 2010
  • Place of Presentation: オーストリア
• [Presentation] Genetic Prion disease in Japan, An analysis based on the Japanese CJD Surveillance, 1999-2009 (2010)
  • Author(s): Ohara M, Sanjo N, Hizume M, Sakai K, Nozaki I, Hamaguchi T, Nakamura Y, Kitamoto T, Shiga Y, Satoh K, Satoh T, Shirabe S, Yamada M, Tateishi J, Mizusawa H
  • Organizer: Prion 2010
  • Place of Presentation: オーストリア
• [Presentation] 進行性の認知機能障害で発症し, 骨髄生検にて診断しえた血管内悪性リンパ腫症(IVL)の1例. (2009)
  • Author(s): 六倉和生, 佐藤克也, 福田卓, 辻野彰, 吉村俊朗, 中村龍文, 本村政勝, 今西大介, 波多智子, 江口勝美.
  • Organizer: 日本内科学会九州地方会
  • Place of Presentation: 北九州
  • Year and Date: 2009-05-30
• [Presentation] 重症筋無力症の嚥下機能評価に関する検討. (2009)
  • Author(s): 枡田智子, 本村政勝, 徳田昌紘, 立石洋平, 石飛進吾, 福田卓, 佐藤克也, 辻野彰, 吉村俊朗, 江口勝美.
  • Organizer: 日本内科学会総会
  • Place of Presentation: 東京
• [Presentation] クロイツフェルト・ヤコブ病(CJD)における血液脳関門(BBB)についての検討 (2009)
  • Author(s): 六倉和生, 佐藤克也, 江口勝美, 調漸, 長郷国彦, 岸田日帯, 黒岩義之, 三條伸夫, 水澤英洋
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
• [Presentation] CJD患者における髄液中の異常プリオン蛋白の検出 (2009)
  • Author(s): 佐藤克也, 調漸, 六倉和生, 江口勝美, 新竜一郎, 西田教行
  • Organizer: 第50回日本神経学会総会
  • Place of Presentation: 仙台
• [Presentation] 当科における重症筋無力症(MG)・胸腺腫に合併した赤芽球癆(PRCA)の5症例 (2009)
  • Author(s): 佐藤克也, 福田卓, 六倉和生, 徳田昌紘, 立石洋平, 坂井(加用)無二子, 枡田智子, 辻野彰, 吉村俊朗, 中村龍文, 本村政勝, 江口勝美.
  • Organizer: 第27回日本神経治療学会総会.
  • Place of Presentation: 熊本
• [Presentation] CJD患者における髄液中の異常プリオン蛋白の検出. (2009)
  • Author(s): 佐藤克也, 調漸, 六倉和生, 江口勝美, 新竜一郎, 西田教行
  • Organizer: 第14回日本神経感染症学会総会
  • Place of Presentation: 栃木
• [Presentation] プリオン病患者脳脊髄液中診断マーカーの比較検討 (2008)
  • Author(s): 佐藤克也, 調漸, 江口勝美
  • Organizer: 日本神経学会総会
  • Place of Presentation: 横浜
• [Presentation] Establishment of Standardization of 14-3-3 protein assay as a Diagnostic Tool in Creutzfeldt-Jakob disease patients' CSF. (2008)
  • Author(s): Satoh K, Tobiume M, MatsuiY, Mutsukura K Nishida N, Shiga Y, Eguhchi K, Shirabe S, Sata T
  • Organizer: NeuroPrion
  • Place of Presentation: Spain
• [Book] Annual Review神経 (2011)
  • Author(s): 新竜一郎、佐藤克也
• [Book] Annual Review神経 (2011)
  • Author(s): 新竜一郎、佐藤克也
  • Publisher: 中外医学出版
• [Book] 検査所見-脳脊髄液-プリオン病と遅発性ウイルス感染症 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
• [Book] プリオン病と遅発性ウイルス感染症 (2010)
  • Author(s): 佐藤克也、調漸、江口勝美
  • Publisher: 金原出版
• [Remarks] ホームページ等
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/cmb/index.html
• [Remarks]
  • URL: http://prion.umin.jp/survey/index.html
• [Remarks]
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/cmb/index.html
• [Remarks]
  • URL: http://prion.umin.jp/survey/index.html
• [Remarks] 厚生労働省難病疾患克服事業 プリオン病及び遅発性ウィルス感染症に関する調査研究 調 漸(分担研究者)佐藤克也(研究協力者)
  • URL: http://prion.umin.jp/