Identification of the novel factors relating to prion infection, and analysis of its molecular mechanisms
| Project/Area Number |
20591002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUSE Takayuki Nagasaki University, 医歯薬学総合研究科, 助教 (30432975)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIDA Noriyuki 長崎大学, 医歯薬学総合研究科, 教授 (40333520)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 感染症 / 神経変性疾患 / プリオン / 精製 / タンパク質 / 神経細胞 / 脳 / 感染機構 / 培養細胞 / 神経細胞死 |
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| Research Abstract |
We can propose that purification and identification of the novel factor relating to prion infection in this study. To purify for prion, we had collected the supernatants or cell extract from prion affected mouse neuronal culture cells, and then, they had been separated to refine by density-gradient centrifugation. The collected fractions had been analyzed the accumulation of PrP^<Sc> and determined its infectivity. Moreover, we had been successful in identification of specific proteins relating to prion infection including 33 or 93 kinds of proteins from the cell extract, or the supernatants in prion affected cells, respectively.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.2011
Author(s)
Atarashi R, Satoh K, Sano K, Fuse T, Yamaguchi N, Ishibashi D, Matsubara T, Nakagaki T, Yamanaka H, Shirabe S, Yamada M, Mizusawa H, Kitamoto T, Klug G, McGlade A, Collins SJ, Nishida N.
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Journal Title
Nature medicine 17(2)
Pages: 175-178
NAID
Related Report
Peer Reviewed
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[Journal Article] Transcytosis of murine-adapted bovine spongiform encephalopathy agents in an in vitro bovine M cell model.2010
Author(s)
Miyazawa K, Kanaya T, Takakura I, Tanaka S, Hondo T, Watanabe H, Rose MT, Kitazawa H, Yamaguchi T, Katamine S, Nishida N, Aso H.
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Journal Title
J Virol 84(23)
Pages: 12285-12291
Related Report
Peer Reviewed
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