| Project/Area Number |
20591007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
TOKUDA Takahiko Kyoto Prefectural University of Medicine, 医学研究科, 講師 (80242692)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Yoshihisa 京都府立医科大学, 医学研究科, 助教 (50363990)
MIZUNO Toshiki 京都府立医科大学, 医学研究科, 准教授 (30264782)
NAKAGAWA Masanori 京都府立医科大学, 医学研究科, 教授 (50198040)
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| Co-Investigator(Renkei-kenkyūsha) |
KASAI Takashi 京都府立医科大学, 医学研究科, 助教 (70516062)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | α-シヌクレイン / ニューロシン / 酵素 / パーキンソン病 / 蛋白質分解 / セリンプロテアーゼ / 蛋白分解 / 培養細胞 / 小胞体 / GFP |
|---|
| Research Abstract |
We investigated proteolytic activity and control factors of human neurosin using cultured cells. Heterologous expression of pre-pro-neurosin was localizedto the endoplasmic reticulum and secreted. Extracellular neurosin had protease activity and can degrade α-synuclein. Meanwhile, intracellular neurosin did not have α-synuclein -degrading activity. These findings suggest that neurosin is involved in degradation ofα-synuclein as an extracellular protease.
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