| Project/Area Number |
20591030
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Showa University |
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Principal Investigator |
WAKAYAMA Yoshihiro Showa University, 医学部, 教授 (40138467)
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| Co-Investigator(Kenkyū-buntansha) |
ARATA Satoru 昭和大学, 遺伝子組換え実験室, 准教授 (20159502)
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| Co-Investigator(Renkei-kenkyūsha) |
JIMI Takahiro 昭和大学, 医学部, 兼任講師 (30196654)
INOUE Masahiko 昭和大学, 医学部, 兼任講師 (50286770)
SHIBUYA Seiji 昭和大学, 医学部, 兼任講師 (80167444)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 臨床神経形態学 / AQP1 / 骨格筋 / 再生 / 血管内皮 / 治療 / AQP1 Tgマウス / アクアポリン1 |
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| Research Abstract |
Adenovirus vector with AQP1 (experimental group : EG) or that with Lac Z (control group : CG) was injected into the skeletal muscle necrotic tissue of normal or mdx mouse hind limb, respectively. The necrotic and regenerative skeletal muscle tissues after 3 days of injection showed that numerous blood vessels with anti-AQP1 antibody immunopositive staining were present, although their numbers appeared not to be different between EG and CG. The AQP1 mRNA levels of muscle regenerative tissues after 3 days of injection were not statistically different between two groups both in normal and mdx mice. Furthermore, AQP1 overexpressing transgenic mouse is now under generation by using LNL-AQP1 expression system. The DNA fragments were injected into the ova of 188 mice and finally we obtained one male mouse with transgene.
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