Analysis of intracellular signaling pathway of an anorexic hormone 'Nesfatin-1'.

• Project/Area Number: 20591045
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Gunma University
• Principal Investigator: HASHIMOTO Koshi Gunma University, 大学院・医学系研究科, 助教 (30396642)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: メタボリックシンドローム / Nesfatin-1 / 摂食抑制 / 遺伝子 / 受容体 / シグナル伝達 / MC4R / G蛋白共役型受容体 / DNAマイクロアレイ / CREB

Research Abstract

Nesfatin-1, which is a novel anorexic hormone, induces phosphorylation of intracellular cAMP response element binding protein (CREB) in MNB cells derived from mouse neuroblastoma. M30, which is a truncated peptide of Nesfatin-1(24-53aa), also exerts a similar effect as Nesfatin-1. Nesfatin-1 specific receptor is located in cell membrane fraction of mouse hypothalamus and MNB cells. Melanocrotin 4 receptor per se and its signaling pathway is essential to the intracellular signaling machinery exerted by Nesfatin-1/M30.

Research Products

• [Journal Article] Fasting concentrations of nesfatin-1 are negatively correlated with body mass index in non-obese males. (2010)
  • Author(s): Tsuchiya T, Shimizu H, Yamada M, Osaki A, Oh-I S, Ariyama Y, Takahashi H, Okada S, Hashimoto K, Satoh T, Kojima M, Mori M
  • Journal Title: Clin Endocrinol (Oxf). 73 Pages: 484-490
  • Peer Reviewed
• [Journal Article] Troglitazone, a ligand of peroxisome proliferator-activated receptor-{gamma}, stabilizes NUCB2 (Nesfatin) mRNA by activating the ERK1/2 pathway : isolation and characterization of the human NUCB2 gene. (2010)
  • Author(s): Yamada M, Horiguchi K, Umezawa R, Hashimoto K, Satoh T, Ozawa A, Shibusawa N, Monden T, Okada S, Shimizu H, Mori M
  • Journal Title: Endocrinology. 151 Pages: 2494-2503
  • Peer Reviewed
• [Journal Article] Troglitazone, a ligand of peroxisome proliferator-activated receptor-γ, stabilizes NUCB2 (Nesfatin) mRNA by activating the ERK1/2 pathway : isolation and characterization of the human NUCB2 gene. (2010)
  • Author(s): Yamada M, Horiguchi K, Urnezawa R, Hashimoto K, Satoh T, Ozawa A, Shibusawa N, Monden T, Okada S, Shimizu H, Mori M.
  • Journal Title: Endocrinology. Volume: 151 Pages: 2494-2503
  • Peer Reviewed
• [Journal Article] Fasting concentrations of nesfatin-1 are negatively correlated with body mass index in non-obese males. (2010)
  • Author(s): Tsuchiya T, Shimizu H, Yamada M, Osaki A, Oh-I S, Ariyama Y, Takahashi H, Okada S, Hashimoto K, Satoh T, Kojima M, Mori M.
  • Journal Title: Clin Endocrinol (Oxf). Volume: 73 Pages: 484-490
  • Peer Reviewed
• [Journal Article] Nesfatin-1-regulated oxytocinergic signaling in the paraventricular nucleus causes anorexia through a leptin-independent melanocortin pathway. (2009)
  • Author(s): Maejima Y, Sedbazar U, Suyama S, Kohno D, Onaka T, Takano E, Yoshida N, Koike M, Uchiyama Y, Fujiwara K, Yashiro T, Horvath TL, Dietrich MO, Tanaka S, Dezaki K, Oh-I S, Hashimoto K, Shimizu H, Nakata M, Mori M, Yada T
  • Journal Title: Cell Metab. 10 Pages: 355-365
  • Peer Reviewed
• [Journal Article] Peripheral administration of nesfatin-1 reduces food intake in mice : the leptin-independent mechanism. (2009)
  • Author(s): Shimizu H, Oh-I S, Hashimoto K, Nakata M, Yamamoto S, Yoshida N, Eguchi H, Kato I, Inoue K, Satoh T, Okada S, Yamada M, Yada T, Mori M
  • Journal Title: Endocrinology. 150 Pages: 662-671
  • Peer Reviewed
• [Journal Article] Nesfatin-1-regulated oxytocinergic signaling in the paraventricular nucleus causes anorexia through a leptin-independent melanocortin pathway. (2009)
  • Author(s): Maejima Y, Sedbazar U, Suyama S, Kohno D, Onaka T, Takano E, Yoshida N, Koike M, Uchiyama Y, Fujiwara K, Yashiro T, Horvath TL, Dietrich MO, Tanaka S, Dezaki K, Oh-IS, Hashimoto K, Shimizu H, Nakata M, Mori M, Yada T.
  • Journal Title: Cell Metab. 10 Pages: 355-365
  • Peer Reviewed
• [Journal Article] Peripheral administration of nesfatin-1 reduces food intake in mice : the leptin-independent mechanism. (2009)
  • Author(s): Shimizu H, Oh-IS, Hashimoto K, Nakata M, Yamamoto S, Yoshida N, Eguchi H, Kato I, Inoue K, Satoh T, Okada S, Yamada M, Yada T, Mori M.
  • Journal Title: Endocrinology 150 Pages: 662-671
  • Peer Reviewed
• [Journal Article] Fort P, Salvert D, Hanriot L, Jego S, Shimizu H, Hashimoto K, Mori M, Luppi PH (2008)
  • Author(s): The satiety molecule nesfatin-1 is co-expressed with melanin concentrating hormone in tuberal hypothalamic neurons of the rat.
  • Journal Title: Neuroscience. 155 Pages: 174-181
  • Peer Reviewed
• [Presentation] チアゾリジン誘導体のNUCB2 mRNA安定性制御機構 (2010)
  • Author(s): 山田正信,堀口和彦,梅澤良平,田口亮,橋本貢士,佐藤哲郎,岡田秀一,清水弘行,森昌朋
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋テルサ(群馬県)
  • Year and Date: 2010-10-02
• [Presentation] 摂食抑制ペプチドnesfatin-1に対する細胞内シグナル伝達とMelanocortin Receptorの関連 (2010)
  • Author(s): 石田恵美、橋本貢士、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第31回日本肥満学会
  • Place of Presentation: 前橋テルサ(群馬県)
  • Year and Date: 2010-10-01
• [Presentation] 摂食抑制ペプチドnesfatin-1の細胞内シグナル伝達機構の解析 (2010)
  • Author(s): 石田恵美、橋本貢士、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山国際交流センター(岡山県)
  • Year and Date: 2010-05-28
• [Presentation] Melanocortin 3/4 receptor is essential to the intracellular signaling pathway of nesfatin-1. (2010)
  • Author(s): Emi Ishida, Koshi Hashimoto, Tetsurou satoh, Shuichi Okada, Masanobu Yamada, Hiroyuki Shimizu, Masatomo Mori
  • Organizer: 14th International Congress of Endocrinology (ICE2010)
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2010-03-30
• [Presentation] Melanocortin 3/4 receptor is essential to the intracellular signaling pathway of nesfatin-1. (2010)
  • Author(s): Emi Ishida, Koshi Hashimoto, Tetsurou Satoh, Shuichi Okada, Masanobu Yamada, Hiroyuki Shimizu, Masatomo Mori
  • Organizer: 14^<th> International Congress of Endocrinology(ICE2010)(第14回国際内分泌学会議)
  • Place of Presentation: Kyoto International Conference Center(国際京都会館)
  • Year and Date: 2010-03-30
• [Presentation] Nesfatin/Nesfatin-1はadpogenesisを抑制する (2009)
  • Author(s): 三浦敦子,岡田秀一,清水弘行,土屋天文,上原豊,橋本貢士,山田正信,高橋洋樹,大崎綾,大島喜八,森昌朋
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(群馬県)
  • Year and Date: 2009-04-23
• [Presentation] 摂食抑制蛋白nesfatin-1の細胞内シグナル伝達とMelanocortin Receptorの関与 (2009)
  • Author(s): 石田恵美、橋本貢士、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(群馬県)
  • Year and Date: 2009-04-23
• [Presentation] 摂食抑制蛋白nesfatin-1の細胞内シグナル伝達とMelanocortin Receptorの関与 (2009)
  • Author(s): 石田恵美、橋本貢士、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第82回日本内分泌学会学術集会
  • Place of Presentation: 群馬県民会館(前橋市)
  • Year and Date: 2009-04-23
• [Presentation] 新規摂食抑制蛋白nesfatin-1の細胞内シグナル伝達経路の解析 (2008)
  • Author(s): 石田恵美、橋本貢士、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第35回日本神経内分泌学会
  • Place of Presentation: 政策研究大学院大学(東京都)
  • Year and Date: 2008-08-29
• [Presentation] 新規摂食抑制蛋白Nesfatin-1の細胞内シグナル伝達経路の解析 (2008)
  • Author(s): 石田恵美
  • Organizer: 第35回日本神経内分泌学会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-29
• [Presentation] 新規摂食抑制蛋白nesfatin-1の細胞内シグナル伝達経路の解析 (2008)
  • Author(s): 石田恵美、橋本貢士、堀口和彦、松本俊一、吉野聡、梅澤良平、中島康代、石塚高広、橋田哲、佐藤哲郎、岡田秀一、山田正信、清水弘行、森昌朋
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: ホテル青森(青森県)
  • Year and Date: 2008-05-16
• [Presentation] ヒトネスファチン1(NUCB2)遺伝子構造と発現制御機構の解析 (2008)
  • Author(s): 梅澤良平,山田正信,堀口和彦,石塚高広,橋田哲,橋本貢士,佐藤哲郎,清水弘行,森昌朋
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: ホテル青森(青森県)
  • Year and Date: 2008-05-16
• [Presentation] 新規摂食抑制蛋白Nesfatin-1の細胞内シグナル伝達経路の解析 (2008)
  • Author(s): 石田恵美
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森
  • Year and Date: 2008-05-16