| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
Preventive effect of human glutamic acid decarboxylase (GAD) 65 (GAD65) vaccination for type 1 diabetes was examined using NOD mice model. Subcutaneous electropolation of site-specific GAD65 fragments including GAD65 (1-83aa : N-GAD65), GAD65 (244-443aa : Center-GAD65) and GAD65 (443-585aa : C-GAD65) were done to 4 week female NOD mice.
Specific T cell responses to N-GAD65, Center-GAD65 and C-GAD65 were observed to respective GAD65 fragments in electropolated NOD mice. However, occurrence rate of diabetes in GAD65 vector- or empty vector- electropolated groups did not reach significant levels during 30 weeks.
Human GAD65 different from mice GAD65 may explain a failure to prevent the occurrence of diabetes by human site-specific GAD65 vaccination.
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