Identification and characterization of a new factor regulating SREBP-1c gene in liver.

• Project/Area Number: 20591058
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Shiga University of Medical Science
• Principal Investigator: NISHIO Yoshihiko Shiga University of Medical Science, 医学部, 准教授 (40281084)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 脂質合成 / 転写調節 / 栄養素 / 脂質代謝 / 肝細胞 / RBMX / 果糖

Research Abstract

We have shown that RBMX and its binding protein SAFB regulate the transcriptional activity of SREBP-1c gene. Furthermore, we have identified a new gene, hepothetical protein LOC 673353, really expressing as mRNA and protein regulating SREBP-1c gene.

Research Products

• [Journal Article] Postprandial activation of protein kinase Cm regulates the expression of adipocytokines via the transcription factor AP-2b. (2011)
  • Author(s): Kondo M, Ugi S, Morino K, Fuke T, Obata T, Yoshizaki T, Nishio Y, Maeda S, Kashiwagi A, Maegawa H.
  • Journal Title: Int J Mol Med.PubMed 28(1) Pages: 95-100
  • Peer Reviewed
• [Journal Article] Postprandial activation of protein kinase Cツオ regulates the expression of adipocytokines via the transcription factor AP-2beta (2011)
  • Author(s): Kondo M, Ugi S, Morino K, Fuke T, Obata T, Yoshizaki2T, Nishio Y, Maeda S, Kashiwagi A, Maegawa H
  • Journal Title: Int J Mol Med Volume: 28 Pages: 95-100
  • Peer Reviewed
• [Journal Article] Relation of the expression of transcriptional factor TFAP2B to that of adipokines in subcutaneous and omental adipose tissues. (2010)
  • Author(s): Ugi S, Nishio Y, Yamamoto H, Ikeda K, Kobayashi M, Tsukada S, Kondo M, Morino K, Obata T, Yoshizaki T, Uzu T, Naitoh H, Tani T, Maeda S, Kashiwagi A, Maegawa H.
  • Journal Title: Obesity (Silver Spring). 18(7) Pages: 1277-1282
  • Peer Reviewed
• [Journal Article] Transcription factor AP-2beta : a negative regulator of IRS-1 gene expression. (2010)
  • Author(s): Meng X, Kondo M, Morino K, Fuke T, Obata T, Yoshizaki T, Ugi S, Nishio Y, Maeda S, Araki E, Kashiwagi A, Maegawa H.
  • Journal Title: Biochem Biophys Res Commun. 19;392(4) Pages: 526-532
  • Peer Reviewed
• [Journal Article] Low concentration of 4-hydroxy hexenal increases heme oxygenase-1 expression through activation of Nrf2 and antioxidative activity in vascular endothelial cells. (2010)
  • Author(s): Ishikado A, Nishio Y, Morino K, Ugi S, Kondo H, Makino T, Kashiwagi A, Maegawa H.
  • Journal Title: Biochem Biophys Res Commun. 5;402(1) Pages: 99-104
  • Peer Reviewed
• [Journal Article] Low concentration of 4-hydroxy hexenal increases heme oxygenase-1 expression through activation of Nrf2 and antioxidative activity in vascular endothelial cells (2010)
  • Author(s): Ishikado A, Nishio Y, Morino K, Ugi S, Kondo H, Makino T, Kashiwagi A, Maegawa H
  • Journal Title: Biochem Biophys Res Commun Volume: 402 Pages: 99-104
  • Peer Reviewed
• [Journal Article] Effects of a Fish-Based Diet on the Serum Adiponectin Concentration in Young, Non-Obese, Healthy Japanese Subjects (2010)
  • Author(s): Kondo K, Morino K, Nishio Y, Kondo M, Fuke T, Ugi S, Iwakawa H, Kashiwagi A, Maegawa H.
  • Journal Title: J Atheroscler Thromb (In press)
  • NAID: 130004845323
  • Peer Reviewed
• [Journal Article] Transcription factor activating protein-2beta : a positive regulator of monocyte chemoattractant protein-1 gene expression. (2009)
  • Author(s): Kondo M, Maegawa H, Obata T, Ugi S, Ikeda K, Morino K, Nakai Y, Nishio Y, Maeda S, Kashiwagi A.
  • Journal Title: Endocrinology. 150(4) Pages: 1654-1661
  • Peer Reviewed
• [Journal Article] Soy phosphatidylcholine inhibited TLR4-mediated MCP-1 expression in vascular cells. (2009)
  • Author(s): Ishikado A, Nishio Y, Yamane K, Mukose A, Morino K, Murakami Y, Sekine O, Makino T, Maegawa H, Kashiwagi A.
  • Journal Title: Atherosclerosis. 205(2) Pages: 404-412
  • Peer Reviewed
• [Journal Article] SAFB1, an RBMX-binding protein, is a newly identified regulator of hepatic SREBP-1c gene. (2009)
  • Author(s): Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A.
  • Journal Title: BMB Rep. 30;42(4) Pages: 232-237
  • Peer Reviewed
• [Journal Article] Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression and consequent hypertriglyceridaemia. (2009)
  • Author(s): Ugi S, Shi K, Nishio Y, Shimizu S, Guo B, Sekine O, Ikeda K, Egawa K, Yoshizaki T, Nagai Y, Koya D, Takada T, Torii R, Kimura H, Kashiwagi A, MaegawaH.
  • Journal Title: J Biochem. 146(4) Pages: 541-547
  • NAID: 10026304029
  • Peer Reviewed
• [Journal Article] Membrane localization of protein-tyrosine phosphatase 1B is essential for its activation of sterol regulatory element-binding protein-1 gene expression and consequent hypertriglyceridaemia (2009)
  • Author(s): Ugi S, Shi K, Nishio Y, Shimizu S, Guo B, Sekine O, Ikeda K, Egawa K, Yoshizaki T, Nagai Y, Koya D, Takada T, Torii R, Kimura H, Kashiwagi A, Maegawa H.
  • Journal Title: J Biochem 146 Pages: 541-547
  • NAID: 10026304029
  • Peer Reviewed
• [Journal Article] Higher liver fat content among Japanese in Japan compared with non-Hispanic whites in the United States (2009)
  • Author(s): Azuma K, Kadowaki T, Cetinel C, Kadota A, El-Saed A, Kadowaki S, Edmundowicz D, Nishio Y, Sutton-Tyrrell K, Okamura T, Evans RW, Takamiya T, Ueshima H, Curb JD, Abbott RD, Kuller LH, Kelley DE, Sekikawa A; ERA JUMP study group
  • Journal Title: Metabolism 58 Pages: 1200-1207
  • Peer Reviewed
• [Journal Article] Transcription factor activating protein-2beta : a positive regulator of monocyte chemoattractant protein-1 gene expression (2009)
  • Author(s): Kondo M, Maegawa H, Obata T, Ugi S, Ikeda K, Morino K, Nakai Y, Nishio Y, Maeda S, Kashiwagi A
  • Journal Title: Endocrinology 150 Pages: 1654-1661
  • Peer Reviewed
• [Journal Article] SAFB1, an RBMX-Binding Protein, is a Newly Identified Regulator of Hepatic SREBP-lc Gene
  • Author(s): Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A
  • Journal Title: BMB reports (In press)
  • Peer Reviewed
• [Presentation] N-3 polyunsaturated fatty acid (n-3 PUFA) increases anti-oxidant enzyme HO-1 and decreases inflammatory cytokine MCP-1 in vascular endothelial cells. (2010)
  • Author(s): Ishikado A, Nishio Y, Morino K, Mukose K, Murakami Y, Makino T, Kashiwagi A, Maegawa H
  • Organizer: 70th annual meeting of American Diabetes Association
  • Place of Presentation: Orlando
• [Presentation] Hyperinsulinemia and atheroscrelosis (2010)
  • Author(s): 西尾善彦, 石角篤、前川聡
  • Organizer: 第53回日本糖尿病学会年次学術集会
  • Place of Presentation: 岡山
• [Presentation] RNA binding motif protein on the X chromosome (RBMX), a novel key molecule for developing metabolic syndrome and NASH. (2009)
  • Author(s): Maeno Y, Nishio Y, Nagai Y, Takemoto T, Sekine O, Omura Y, Ushio M, Kimura H, Kashiwagi A, Maegawa H
  • Organizer: 14th International Congress of Endocrinology
  • Place of Presentation: Kyoto
• [Presentation] フルクトースによる脂質合成促進の分子機構 (2009)
  • Author(s): 西尾善彦, 前川聡, 柏木厚典
  • Organizer: 第12回日本病態栄養学会
  • Place of Presentation: 京都
• [Presentation] 糖尿病合併症の発症機構と歯周病との関連について (2009)
  • Author(s): 西尾善彦, 前川聡, 柏木厚典
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
• [Presentation] 高インスリン血症と血管炎症における転写因子の役割 (2009)
  • Author(s): 西尾善彦, 前川聡, 柏木厚典
  • Organizer: 第24回日本糖尿病合併症学会
  • Place of Presentation: 岡山
• [Presentation] A Newly Identified Protein, LOC673353, Activates the Promoter of SREBP-1c Gene through Direct Binding to Its Promoter and Association with a Co-Activator, RBMX. (2008)
  • Author(s): Sekine O, Nishio Y, Ikeuchi C, Takemoto T, Quynh N, Ugi S, Maegawa H, Kimura H, Kashiwagi A
  • Organizer: 68th annual meeting of American Diabetes Association
  • Place of Presentation: San Francisco
• [Presentation] Activation of Transcription Factor AP-2β Mediated by Protein Kinase C μ Aggravates Expression of Inflammatory Adipokines in Differentiated Adipocyte. (2008)
  • Author(s): Kondo M, Maegawa H, Morino K, Fuke T, Obata T, Ugi S, Nishio Y, Maeda S, Kashiwagi A
  • Organizer: 68th annual meeting of American Diabetes Association
  • Place of Presentation: San Francisco
• [Presentation] Safb1, an RBMX-Binding Protein, is a Newly Identified Activator in the Regulation of Hepatic SREBP-1 Gene Expression. (2008)
  • Author(s): Omura Y, Nishio Y, Takemoto T, Ikeuchi C, Sekine O, Morino K, Maeno Y, Obata T, Ugi S, Maegawa H, Kimura H, Kashiwagi A
  • Organizer: 68th annual meeting of American Diabetes Association
  • Place of Presentation: San Francisco
• [Presentation] The molecular mechanism of atherosclerosis induced by hyperinsulinemia (2008)
  • Author(s): 西尾善彦, 前川聡, 柏木厚典
  • Organizer: 第40回日本動脈硬化学会総会
  • Place of Presentation: 筑波
• [Presentation] 果糖反応性SREBP-1c遺伝子発現に関する新しい転写調節因子群の同定 (2008)
  • Author(s): 西尾善彦, 前川聡, 柏木厚典
  • Organizer: 第29回日本肥満学会
  • Place of Presentation: 大分