Mechanism of pancreatic beta cell expansion by bone marrow transplantation

• Project/Area Number: 20591073
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Metabolomics
• Research Institution: Juntendo University
• Principal Investigator: WATADA Hirotaka Juntendo University, 医学部, 教授 (60343480)
• Co-Investigator(Kenkyū-buntansha): YOSHIO Fujitani 順天堂大学, 医学研究科, 准教授 (30433783)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 膵β細胞 / 糖尿病 / 骨髄移植 / VEGF-A / 血管内皮

Research Abstract

Bone marrow transplantation increases beta cell mass, however its underlying mechanism has not been elucidated yet. In this study, we investigated its mechanism. Bone marrow transplantation experiments under several conditions revealed that systemic irradiation without injection of bone marrow cells can increase beta cell mass. On the other hand, irradiation only to bone marrow or only to pancreas cannot increase beta cell mass. In addition, using beta cell specific VEGF-A knock out mice, we found that VEGF-A expression in beta cells are essential for the increase in beta cell mass by bone marrow transplantation. Accordingly, systemic irradiation is essential and sufficient for bone marrow transplantation induced increase in beta cell mass and VEGF-A in beta cells are also essential for this phenomenon.

Research Products

• [Journal Article] Role of VEGF-A in pancreatic beta cells. (2010)
  • Author(s): Watada H
  • Journal Title: Endocr J. 57(3) Pages: 185-91
  • NAID: 10029583836
• [Journal Article] Role of VEGF-A in pancreatic beta cells. (2010)
  • Author(s): Watada H.
  • Journal Title: Endocr J. Volume: 57(3) Pages: 185-91
  • NAID: 10029583836
  • Peer Reviewed
• [Journal Article] Normal islet vascularization is dispensable for expansion of beta-cell mass in response to high-fat diet induced insulin resistance. (2009)
  • Author(s): Toyofuku Y, Uchida T, Nakayama S, Hirose T, Kawamori R, Fujitani Y, Inoue M, Watada H
  • Journal Title: Biochem Biophys Res Commun. 383(3) Pages: 303-7
• [Journal Article] Impact of whole body irradiation and vascular endothelial growth factor-A on increased beta cell mass after bone marrow transplantation in a mouse model of diabetes induced by streptozotocin. (2009)
  • Author(s): Nakayama S, Uchida T, Choi JB, Fujitani Y, Ogihara T, Iwashita N, Azuma K, Mochizuki H, Hirose T, Kawamori R, Inoue M, Watada H
  • Journal Title: Diabetologia. 52(1) Pages: 115-24
• [Journal Article] Normal islet vascularization is dispensable for expansion of beta-cell mass in response to high-fat diet induced insulin resistance (2009)
  • Author(s): Toyofuku Y, et al.
  • Journal Title: Biochem Biophys Res Commun 383 Pages: 303-307
  • Peer Reviewed
• [Journal Article] Impact of whole body irradiation and vascular endothelial growth factor-A on increased beta cell mass after bone marrow transplant ation in a mouse model of diabetes indeuce by streptozotocin (2009)
  • Author(s): Nakayama S., et al.
  • Journal Title: Diabetologia 52 Pages: 115-124
  • Peer Reviewed
• [Presentation] 高脂肪食負荷時の膵β細胞量調節におけるVEGF-Aの関与 (2009)
  • Author(s): 豊福優希子, ら
  • Organizer: 第52回日本糖尿病学会年次学術集会
  • Place of Presentation: 大阪
• [Remarks] ホームページ等