Functional analysis of ACTH receptor (MC2R) signal using MC2R knockout mouse

• Project/Area Number: 20591086
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Endocrinology
• Research Institution: Hokkaido University
• Principal Investigator: SHIMIZU Chikara Hokkaido University, 病院, 講師 (00292029)
• Co-Investigator(Kenkyū-buntansha): MIYOSHI Hideaki 北海道大学, 病院, 講師 (30360902)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ACTH受容体 / 遺伝子破壊マウス / シグナル

Research Abstract

It is well-known that hypothalamic-pituitary-adrenal axis has an apparent circadian rhythm. We evaluated the circadian behavioral variation on MC2R deficient mice. There was no difference on behavioral rhythms under light-dark cycle between wild-type mice and MC2R knockout mice. Behavioral pattern under constant darkness is now under investigation.

Research Products

• [Journal Article] Stable isotope-dilution liquid chromatography/tandem mass spectrometry method for determination of thyroxine in saliva. (2011)
  • Author(s): Higashi T, Ichikawa T, Shimizu C, Nagai S, Inagaki S, Min JZ, Chiba H, Ikegawa S, Toyo'oka T.
  • Journal Title: J Chromatogr B Analyt Technol Biomed Life Sci. 879 Pages: 1013-1017
  • Peer Reviewed
• [Journal Article] The incidence and mechanism of sunitinib-induced thyroid atrophy in patients with metastatic renal cell carcinoma. (2011)
  • Author(s): Shinohara N, Takahashi M, Kamishima T, Ikushima H, Otsuka N, Ishizu A, Shimizu C, Kanayama H, Nonomura K.
  • Journal Title: Br J Cancer. 104 Pages: 241-247
  • NAID: 120003169038
  • Peer Reviewed
• [Journal Article] The incidence and mechanism of sunitinib-induced thyroid atrophy in patients with metastatic renal cell carcinoma (2011)
  • Author(s): Shinohara N, Takahashi M, Kamishima T, Ikushima H, Otsuka N, Ishizu A, Shimizu C, Kanavama H, Nonomura K
  • Journal Title: Br J Cancer Volume: 104 Pages: 1241-1247
  • NAID: 120003169038
  • Peer Reviewed
• [Journal Article] Problems in diagnosing atypical Gitelman's syndrome presenting with normomagnesaemia. (2010)
  • Author(s): Nakamura A, Shimizu C, Nagai S, Yoshida M, Aoki K, Kondo T, Miyoshi H, Wada N, Tajima T, Terauchi Y, Yoshioka N, Koike T.
  • Journal Title: Clin Endocrinol (Oxf). 72 Pages: 272-276
  • Peer Reviewed
• [Journal Article] Levels of Transferrin in Bronchoalveolar Lavage Fluid in Sarcoidosis. (2010)
  • Author(s): Shigemura M, Nasuhara Y, Konno S, Hattori T, Shimizu C, Matsuno K, Nishimura M.
  • Journal Title: Lung. 188 Pages: 151-157
  • NAID: 120002834677
  • Peer Reviewed
• [Journal Article] Perilipin overexpression in mice protects against diet-induced obesity. (2010)
  • Author(s): Miyoshi H, Souza SC, Endo M, Sawada T, Perfield JW 2nd, Shimizu C, Stancheva Z, Nagai S, Strissel KJ, Yoshioka N, Obin MS, Koike T, Greenberg AS.
  • Journal Title: J Lipid Res. 51 Pages: 975-982
  • NAID: 120005906858
  • Peer Reviewed
• [Journal Article] Impact of free-living amoebae on presence of Parachlamydia acanthamoebae in the hospital environment and its survival in vitro without requirement for amoebae. (2010)
  • Author(s): Fukumoto T, Matsuo J, Hayashi M, Oguri S, Nakamura S, Mizutani Y, Yao T, Akizawa K, Suzuki H, Shimizu C, Matsuno K, Yamaguchi H.
  • Journal Title: J Clin Microbiol. 48 Pages: 3360-5
  • NAID: 120003005548
  • Peer Reviewed
• [Journal Article] The role of atopy in the clinical course of pulmonary sarcoidosis in the Japanese population. (2010)
  • Author(s): Hattori T, Konno S, Takahashi A, Isada A, Shigemura M, Matsuno K, Shimizu C, Hizawa N, Yamaguchi E, Nishimura M.
  • Journal Title: Allergy Asthma Proc. 31 Pages: 238-243
  • Peer Reviewed
• [Journal Article] Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype. (2010)
  • Author(s): Sawada T, Miyoshi H, Shimada K, Suzuki A, Okamatsu-Ogura Y, Perfield JW 2nd, Kondo T, Nagai S, Shimizu C, Yoshioka N, Greenberg AS, Kimura K, Koike T.
  • Journal Title: PLoS One. 5
  • NAID: 120002646887
  • Peer Reviewed
• [Journal Article] Ciliates rapidly enhance frequency of conjugation between Escherichia coli strains through bacterial accumulation in vesicles. (2010)
  • Author(s): Matsuo J, Oguri S, Nakamura S, Hanawa T, Fukumoto T, Hayashi Y, Kawaguchi K, Mizutani Y, Yao T, Akizawa K, Suzuki H, Shimizu C, Matsuno K, Kamiya S, Hiroyuki Yamaguchi H.
  • Journal Title: Research in microbiology. 161 Pages: 711-719
  • NAID: 120002646844
  • Peer Reviewed
• [Journal Article] Perilipin overexpression in white adipose tissue induces a brown fat-like phenotype (2010)
  • Author(s): Sawada T, Miyoshi H, Shimada K, Suzuki A, Okamatsu-Ogura Y, Perfield JW 2nd, Kondo T, Nagai S, Shimizu C, Yoshioka N, Greenberg AS, Kimura K, Koike T
  • Journal Title: PLoS One Volume: 5
  • NAID: 120002646887
  • Peer Reviewed
• [Journal Article] Mutation analysis of the SDHB and SDHD genes in pheochromocytomas and paragangliomas : identification of a novel nonsense mutation (Q16 8X) in the SDHB gene (2010)
  • Author(s): Oishi Y, Nagai S, Yoshida M, Fujisawa SI, Sazawa A, Shinohara N, Nonomura K, Matsuno K, Shimizu C
  • Journal Title: Fndocr J Volume: 57 Pages: 745-750
  • NAID: 10029586061
  • Peer Reviewed
• [Journal Article] Right ventricular diastolic dysfunction in patients with left ventricular hypertrophy : analysis of right ventricular myocardial relaxation using two-dimentional speckle tracking imaging. (2009)
  • Author(s): Kaga S, Mikami T, Onozuka H, Omotehara S, Abe A, Yamada S, Okada M, Komatsu H, Inoue M, Yokoyama S, Nishida M, Shimizu C, Matsuno K, Tsutsui H.
  • Journal Title: J Echocardiogr. 7 Pages: 25-33
  • Peer Reviewed
• [Journal Article] Angiotensin II receptor Blocker, Valsartan, Increses Myocardial Blood Volume and Regresses Hypertrophy in Hypertensive Patients. (2009)
  • Author(s): Komatsu H, Yamada S, Iwano H, Okada M, Onozuka H, Mikami T, Yokoyama S, Inoue M, Kaga S, Nishida M, Shimizu C, Matsuno K, Tsutsui H.
  • Journal Title: Circulation J. 73 Pages: 2098-2103
  • Peer Reviewed
• [Journal Article] Simultaneous Determination of Twelve Tetrahydrocorticosteroid Glucuronides in Human Urine by Liquid Chromatography/ Electrospray Ionization-Linear Ion Trap Mass Spectrometry. (2009)
  • Author(s): Ikegawa S, Hasegawa M, Okihara R, Shimizu C, Chiba H, Iida T, Mitamura K.
  • Journal Title: Anal Chem. 81 Pages: 10124-10135
  • Peer Reviewed
• [Journal Article] Simultaneous Determination of Twelve Tetrahydrocorticosteroid Glucuronides in Human Urine by Liquid Chromatography/Electrospray Ionization-Linear Ion Trap Mass Spectrometry (2009)
  • Author(s): Ikegawa S, Hasegawa M, Okihara R, Shimizu C, Chiba H, Iida T, Mitamura K
  • Journal Title: Anal.Chem 81 Pages: 10124-10135
  • Peer Reviewed
• [Journal Article] Angiotensin II receptor Blocker, Valsartan, Increses Myocardial Blood Volume and Regresses Hypertrophy in Hypertensive Patients (2009)
  • Author(s): Komatsu H, Yamada S, Iwano H, Okada M, Onozuka H, Mikami T, Yokoyama S, Inoue M, Kaga S, Nishida M, Shimizu C, Matsuno K, Tsutsui H.
  • Journal Title: Circulation J 73 Pages: 2098-2103
  • Peer Reviewed
• [Journal Article] Right ventricular diastolic dysfunction in patients with left ventricular hypertrophy : analysis of right ventricular myocardial relaxation using two-dimentional speckle tracking imagine (2009)
  • Author(s): Kaga S, Mikami T, Onozuka H, Omotehara S, Abe A, Yamada S, Okada M, Komatsu H, Inoue M, Yokoyama S, Nishida M, Shimizu C, Matsuno K, Tsutsui H
  • Journal Title: J Echocardiogr 7 Pages: 25-33
  • Peer Reviewed
• [Journal Article] The potential role of macrophage migration inhibitory factor on the migration of vascular smooth muscle cells. (2008)
  • Author(s): Okamoto T, Atsumi T, Shimizu C, Yoshioka N, Koike T.
  • Journal Title: J Atheroscler Thromb. 15 Pages: 13-19
  • NAID: 10022607656
  • Peer Reviewed
• [Journal Article] The potential role of macrophage migration inhibitory factor on the migration of vascular smooth muscle cells (2008)
  • Author(s): Okamoto T, Atsumi T, Shimizu C, Yoshioka N, Koike T
  • Journal Title: J Atheroscler Thromb 15 Pages: 13-19
  • NAID: 10022607656
  • Peer Reviewed
• [Journal Article] Adipose triglyceride lipase regulates basal lipolysis and lipid droplet size in adipocytes (2008)
  • Author(s): Miyoshi H, Perfield JW 2nd, Obin MS, Greenberg AS
  • Journal Title: J Cell Biochem 105 Pages: 1430-1436
  • Peer Reviewed
• [Journal Article] AMP-activated Protein Kinase Is Activated as a Consequence of Lipolysis in the Adipocyte : POTENTIAL MECHANISM AND PHYSIOLOGICAL RELEVANCE (2008)
  • Author(s): Gauthier MS, Miyoshi H, Souz a SC, Cacicedo JM, Saha AK, Greenberg AS, Ruderman NB
  • Journal Title: J Biol Chem 283 Pages: 16514-16524
  • NAID: 120006352222
  • Peer Reviewed
• [Presentation] Mutation Analysis of SDHB and SDHD gene in pheochromocytomas and paragangliomas : identification of a novel nonsense mutation(Q168X) (2010)
  • Author(s): Shimizu S, Oishi Y, Nagai S, Yoshida M, S Fujisawa S, Sazawa A, Shinohara N, Nonomura K, Matsuno K
  • Organizer: 14^<th> International Congress of Endocrinology(ICE2010)
  • Place of Presentation: Kyoto
  • Year and Date: 2010-03-29
• [Presentation] Mutation Analysis of SDHB and SDHD gene in pheochromocytomas and paragangliomas : identification of a novel nonsense mutation (Q168X) (2010)
  • Author(s): 清水力, et al.
  • Organizer: 14^<th> International Congress of Endocrinology (ICE2010)
  • Place of Presentation: Kyoto, Japan
• [Presentation] 甲状腺機能と血中アルブミン(alb)濃度との関連性 (2010)
  • Author(s): 清水力
  • Organizer: 第83回日本内分泌学会学術総会
  • Place of Presentation: 京都
• [Presentation] 著明な下腿浮腫および低アルブミン血症を伴ったGraves病の一例-甲状腺機能と血中アルブミン濃度との関連性 (2009)
  • Author(s): 清水力, 他
  • Organizer: 第56回日本臨床検査医学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Study of Administration Interval of Octreotide LAR in the Treatment of Acromegaly (2009)
  • Author(s): 清水力, et al.
  • Organizer: 91th Annual Meeting of the Endocrine Society (ENDO2009)
  • Place of Presentation: Washington DC, USA
• [Presentation] 先端巨大症治療におけるオクトレオチドLAR投与間隔延長の可能性について (2008)
  • Author(s): 清水力, 他
  • Organizer: 第8回日本内分泌学会北海道地方会
  • Place of Presentation: 札幌
  • Year and Date: 2008-10-18
• [Presentation] 成人成長ホルモン分泌不全症患者におけるメタボリックシンドローム構成因子に関する検討 (2008)
  • Author(s): 清水力, 他
  • Organizer: 第55回日本臨床検査医学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] 先端巨大症治療におけるオクトレオチドLAR投与間隔延長の試み (2008)
  • Author(s): 清水力, 他
  • Organizer: 第81回日本内分泌学会学術総会
  • Place of Presentation: 青森