| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Research Abstract |
The exocytosis by anterior pituitary cells were visualized by TEP technique using two-photon excitation. The spontaneous exocytosis by rat lactotroph was rapid full fusion that terminated within a second while GHRH-stimulated exocytosis by rat somatotroph was slower full fusion that terminated within 3-6 seconds. D2 agonist and blockers of voltage-gated calcium channels inhibited spontaneous exocytosis, indicating that the observed spontaneous exocytosis corresponds to the spontaneous prolactin secretion from anterior pituitary when liberated from hypothalamic inhibition by dopamine. Somatostatin, that is a physiological hypothalamic inhibitor of GH secretion, attenuated GHRH-induced exocytosis. Somatostatin also inhibited high K-induced GH exocytosis. Because somatostatin did not abolish high K-induced intracellular calcium increase, somatostatin may inhibit GH exocytosis by acting on distal apparatus to calcium influx. The characteristics of the distal mechanism was investigated further.
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