Visualization of exocytosis by anterior pituitary cells.
| Project/Area Number |
20591088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKANO Koji The University of Tokyo, 医学部附属病院, 特任講師 (20236243)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 下垂体 / ホルモン分泌 / 開口分泌 / 2光子励起法 / 下垂体細胞 / 機能性内分泌腫瘍 |
|---|
| Research Abstract |
The exocytosis by anterior pituitary cells were visualized by TEP technique using two-photon excitation. The spontaneous exocytosis by rat lactotroph was rapid full fusion that terminated within a second while GHRH-stimulated exocytosis by rat somatotroph was slower full fusion that terminated within 3-6 seconds. D2 agonist and blockers of voltage-gated calcium channels inhibited spontaneous exocytosis, indicating that the observed spontaneous exocytosis corresponds to the spontaneous prolactin secretion from anterior pituitary when liberated from hypothalamic inhibition by dopamine. Somatostatin, that is a physiological hypothalamic inhibitor of GH secretion, attenuated GHRH-induced exocytosis. Somatostatin also inhibited high K-induced GH exocytosis. Because somatostatin did not abolish high K-induced intracellular calcium increase, somatostatin may inhibit GH exocytosis by acting on distal apparatus to calcium influx. The characteristics of the distal mechanism was investigated further.
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Report
(4 results)
Research Products
(58 results)
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[Journal Article] 異型性下垂体腺腫と下垂体癌に対するテモゾロミドの有効性と不応性2010
Author(s)
村上峰子,水谷晃子,中口博,山田昌興,保谷克己,浅野修一郎,松野彰,片上秀喜,山崎一人,石田康生,小沢義典,高野幸路,高野順子,佐伯直勝,冲永寛子
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Journal Title
日本内分泌学会雑誌 86巻Suppl
Pages: 40-42
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