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Analyses of tissue-specific action of SERMs using real-time imaging techniques

Research Project

Project/Area Number 20591098
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKyushu University

Principal Investigator

KAWATE Hisaya  Kyushu University, 大学病院, 助教 (20336027)

Co-Investigator(Kenkyū-buntansha) 高柳 涼一  九州大学, 医学研究院, 教授 (30154917)
Co-Investigator(Renkei-kenkyūsha) TAKAYANAGI Ryoichi  九州大学, 大学院・医学研究院, 教授 (30154917)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords核内受容体 / ホルモン / 共焦点顕微鏡 / GFP / 乳癌 / 核小体
Research Abstract

To clarify the molecular mechanism underlying the tissue specificity of SERMs, we examined the intracellular localization of ERα using a green fluorescent protein (GFP)-tagged protein in culture cells from various tissues. Although ERα formed intranuclear foci in the presence of estradiol (E2), RLX translocated ER_into the nucleoli in breast cancer cell lines. This phenomenon was not observed in cells from other tissues. Immunofluorescence staining revealed that endogenous ERα was also translocated into the nucleoli in the presence of RLX. Mutation analyses demonstrate that helix 12 of ERα is essential to the nucleolar translocation of ERα. These results suggest that translocation of ERα into the nucleoli is RLX-specific and is a key event for RLX-induced growth repression of mammary gland cells.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (30 results)

All 2011 2010 2009 2008 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results) Presentation (12 results) Remarks (5 results)

  • [Journal Article] Hypophosphatemic osteomalacia as a long-term complication after liver transplantation.2011

    • Author(s)
      Kawate H, Taketomi A, Watanabe T
    • Journal Title

      Transplantation 91巻1号

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Hypophosphatemic osteomalacia as a long-term complication after liver transplantation.2011

    • Author(s)
      Kawate H, et al
    • Journal Title

      Transplantation

      Volume: 91

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alendronate improves QOL of post-menopausal women with osteoporosis.2010

    • Author(s)
      Kawate H, Ohnaka K, Adachi M
    • Journal Title

      Clin Intery Aging 5巻

      Pages: 123-131

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Raloxifene induces nucleolar translocation of the estrogen receptor.2010

    • Author(s)
      Gushima M, Kawate H, Ohnaka K, Takayanagi R
    • Journal Title

      Mol Cell Endocrinol 319巻1-2号

      Pages: 14-22

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] リアルタイムイメージングによるステロイドホルモン受容体の機能解析2010

    • Author(s)
      河手久弥、高柳涼一
    • Journal Title

      内分泌・糖尿病・代謝内科 30巻5号

      Pages: 492-501

    • NAID

      40017666399

    • Related Report
      2010 Final Research Report
  • [Journal Article] Raloxifene induces nucleolar translocation of the estrogen Receptor.2010

    • Author(s)
      Gushima M, et al.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 319 Pages: 14-22

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relation of cigarette smoking, alcohol use, and coffee consumption to glycated hemoglobin in Japanese men and women.2010

    • Author(s)
      Maki T, et al
    • Journal Title

      Diabetes and Metabolic Syndrome : Clinical Research and Reviews

      Volume: 4 Pages: 69-73

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Relation of coffee consumption and serum liver enzymes in Japanese men and women with reference to effect modification of alcohol use and body mass index.2010

    • Author(s)
      Ikeda M, et al
    • Journal Title

      Scandinavian Journal of Clinical and Laboratory Investigation

      Volume: 70 Pages: 171-179

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Alendronate improves QOL of post-menopausal women with osteoporosis2010

    • Author(s)
      Kawate H, et al
    • Journal Title

      Clinical Intervention in Aging

      Volume: 5 Pages: 123-131

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of single nucleotide polymorphisms in secreted frizzled-related protein 1 gene with bone mineral density in Japanese women2009

    • Author(s)
      Ohnaka K, et al.
    • Journal Title

      Geriatr Gerontol Int. 9

      Pages: 304-309

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel synthetic androgen receptor ligand, S42, works as a selective androgen receptor modulator and possesses metabolic effects with little impact on the prostate2009

    • Author(s)
      Min L, et al.
    • Journal Title

      Endocrinology 150

      Pages: 5606-5616

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The lack of the C-terminal domain of adipose triglyceride lipase causes neutral lipid storage disease through impaired interactions with lipiddroplets.2008

    • Author(s)
      Kobayashi K., et al.
    • Journal Title

      J Clin Endocrinol Metab 93

      Pages: 2877-2884

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Methylation of a conserved intronic CpG island of mouse SF-1 is associated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression.2008

    • Author(s)
      Shirohzu H., et al.
    • Journal Title

      Biochem Biophys Res Commun 369

      Pages: 862-867

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Hypophosphatemic osteomalacia in patients receiving long-term treatment with anti-hepatitis B virus agente.2010

    • Author(s)
      Kawate H, et al
    • Organizer
      14th Asia-Oceania Congress of Endocrinology
    • Place of Presentation
      マレーシア・クアラルンプール
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report
  • [Presentation] Raloxifene induces nucleolar translocation of the estrogen receptor.2010

    • Author(s)
      具島三佳, 河手久弥, 大中佳三
    • Organizer
      The Endocrine Society's 92nd Annual Meeting
    • Place of Presentation
      San Diego, USA
    • Year and Date
      2010-06-21
    • Related Report
      2010 Final Research Report
  • [Presentation] Raloxifene Induces Nucleolar Translocation of the Estrogen Receptor2010

    • Author(s)
      Gushima M, et al
    • Organizer
      The Endocrine Society's 92nd Annual Meeting
    • Place of Presentation
      米国・サンディエゴ
    • Year and Date
      2010-06-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Hypophosphatemic osteomalacia as a long-term complication after liver transplantation2010

    • Author(s)
      河手久弥
    • Organizer
      14th International Congress of Endocrinology(ICE2010)
    • Place of Presentation
      Kyoto
    • Year and Date
      2010-03-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] 肝移植後の長期合併症としての低リン血症性骨軟化症2009

    • Author(s)
      河手久弥
    • Organizer
      第27回肝移植研究会
    • Place of Presentation
      三島
    • Year and Date
      2009-07-11
    • Related Report
      2009 Annual Research Report
  • [Presentation] リアルタイムイメージングによる性ステロイドホルモン受容体の機能解析2009

    • Author(s)
      河手久弥、具島三佳、大中佳三
    • Organizer
      第82回日本内分泌学会学術総会
    • Place of Presentation
      前橋
    • Year and Date
      2009-04-25
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] ラロキシフェンで誘導されるエストロゲン受容体の核小体移行2008

    • Author(s)
      具島三佳、河手久弥、大中佳三
    • Organizer
      第16回日本ステロイドホルモン学会
    • Place of Presentation
      福井
    • Year and Date
      2008-11-22
    • Related Report
      2010 Final Research Report
  • [Presentation] ラロキシフェンで誘導されるエストロゲン受容体の核小体移行2008

    • Author(s)
      具島 三佳
    • Organizer
      第16回ステロイドホルモン学会
    • Place of Presentation
      福井
    • Year and Date
      2008-11-22
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラロキシフェンで誘導されるエストロゲン受容体の核小体移行2008

    • Author(s)
      河手久弥、大中佳三、足立雅広、高柳涼一
    • Organizer
      第50回日本老年医学会学術集会
    • Place of Presentation
      千葉
    • Year and Date
      2008-06-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 骨粗霧症治療薬ラロキシフェンで誘導されるエストロゲン受容体の核小体移行に関する解析2008

    • Author(s)
      河手 久弥
    • Organizer
      第50回日本老年医学会学術集会
    • Place of Presentation
      幕張
    • Year and Date
      2008-06-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラロキシフェンで誘導されるエストロゲン受容体の核小体移行2008

    • Author(s)
      河手 久弥
    • Organizer
      第81回日本内分泌学会学術集会
    • Place of Presentation
      青森
    • Year and Date
      2008-05-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] ラロキシフェンで誘導されるエストロゲン受容体の核小体移行2008

    • Author(s)
      河手久弥、具島三佳、高柳涼一
    • Organizer
      第81回日本内分泌学会学術総会
    • Place of Presentation
      青森
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://www.med.kyushu-u.ac.jp/intmed3/

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/index.html

    • Related Report
      2010 Final Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://hyoka.ofc.kyushu-u.ac.jp/search/index.html

    • Related Report
      2009 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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