Cell therapy using adipose tissue-derived mesenchymal stem cells for primary myelofibrosis

• Project/Area Number: 20591118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Nagoya University
• Principal Investigator: NAKAYAMA Takayuki Nagoya University, 医学部附属病院, 医員 (00456659)
• Co-Investigator(Renkei-kenkyūsha): YAMAMOTO Kouji 名古屋大学, 医学部附属病院, 講師 (90362251)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 脂肪由来間葉系幹細胞 / 造血微小環境 / ケモカイン / 再生医学 / 細胞療法

Research Abstract

Mesenchymal stem cells (MSCs) have emerged as a new therapeutic modality for reconstituting the hematopoietic microenvironment. However, the availability of conventional bone marrow (BM)-derived MSCs (BMSCs) is limited. Recent studies showed that a large number of MSCs could be easily isolated from fat tissue (adipose tissue-derived MSCs [ADSCs]). We extensively evaluated the hematopoiesis-supporting properties of mouse ADSCs. Mouse ADSCs injected into the BM cavity of fatally irradiated mice reconstituted hematopoiesis more promptly than mouse BMSCs and subsequently rescued mice that had received a low number of HSCs. Human ADSCs possessed the higher hematopoiesis-supporting properties than human BMSCs as well.

Research Products

• [Journal Article] Adipose Tissue-Derived Mesenchymal Stem Cells Facilitate Hematopoiesis In Vitro and In Vivo. (2010)
  • Author(s): 中尾典彦、中山享之、八幡崇、六車ゆかり、齋藤繁紀、宮田泰彦、山本晃士、直江知樹
  • Journal Title: The American Journal of Pathology 177(2) Pages: 547-554
  • Peer Reviewed
• [Journal Article] Adipose Tissue-Derived Mesenchymal Stem Cells Facilitate Hematopoiesis In Vitro and In Vivo (2010)
  • Author(s): 中尾典彦、中山享之、直江知樹
  • Journal Title: The American Journal of Pathology Volume: 177(2) Pages: 547-554
  • Peer Reviewed
• [Presentation] 脂肪組織由来間葉系幹細胞は、造血支持機能において骨髄由来のものより優れている (2009)
  • Author(s): 中山享之
  • Organizer: 第71回日本血液学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-23
• [Presentation] 脂肪組織由来間葉系幹細胞は、造血支持機能において骨髄由来のものより優れている。 (2009)
  • Author(s): 中山享之、中尾典彦、直江知樹
  • Organizer: 日本血液学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-23
• [Presentation] Adipocyte-derived stromal cells facilitate hematopoiesis in vitro and in vivo: advantages over bone marrow-derived stromal cells (2008)
  • Author(s): 中尾典彦
  • Organizer: 50th Annual Meeting of American society of Hematology
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2008-12-07
• [Presentation] 脂肪由来間葉系幹細胞における造血支持能力の検討 (2008)
  • Author(s): 中尾典彦
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2008-10-11