Development of cell-based therapy to promote engraftment after cord blood transplantation

• Project/Area Number: 20591156
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Nihon University
• Principal Investigator: MUGISHIMA Hideo Nihon University, 医学部, 教授 (80183648)
• Co-Investigator(Kenkyū-buntansha): MATSUMOTO Taro 日本大学, 医学部, 教授 (50366580) ; ISHIGE Mika 日本大学, 医学部, 助教 (90420950)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 造血幹細胞 / 臍帯血移植 / 脱分化脂肪細胞 / GVHD / 生着不全 / 脂肪細胞 / 造血微小環境 / ストローマ細胞 / 骨芽細胞 / 細胞治療

Research Abstract

We examined that the effect of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on reconstruction of damaged bone marrow microenvironment and engraftment after human umbilical cord blood transplantation in mice. The results showed that intravenous transplantation of DFAT cells promoted recovery of bone marrow hematopoiesis in C56BL/6 mice after radiation-induced myeloablation. Co-transplantation of DFAT cells with human umbilical cord blood hematopoietic stem cells in irradiated immunodeficient (NOD-SCID) mice resulted in an increase number of human CD45-positive hematopoietic cells in bone marrow and peripheral blood at 4 and 12 weeks after the transplantation. These results indicate that DFAT cell transplantation promotes recovery of hematopoiesis in irradiated mice and promote engraftment of human hematopoietic cells after umbilical cord blood transplantation.

Research Products

• [Journal Article] Appropriate doses of granulocyte-colony stimulating factor reduced atherosclerotic plaque formation and increased plaque stability in cholesterol-fed rabbits. (2010)
  • Author(s): Matsumoto T, Watanabe H, Mugishima H, 他
  • Journal Title: Journal of Atherosclerosis and Thrombosis 17(1) Pages: 84-96
  • NAID: 130004444375
  • Peer Reviewed
• [Journal Article] Low invasive angiogenic therapy for myocardial infarction by retrograde transplantation of mononuclear cells expressing the VEGF gene. (2010)
  • Author(s): Hagikura K, Matsumoto T, Mugishima H, 他
  • Journal Title: International Journal of Cardiology 142(1) Pages: 56-64
  • Peer Reviewed
• [Journal Article] Mature adipocyte derived dedifferentiated fat cells can differentiate into smooth muscle-like cells and contribute to bladder tissue regeneration. (2009)
  • Author(s): Sakuma T, Matsumoto T, Mugishima H, 他
  • Journal Title: Journal of Urology 182(1) Pages: 355-365
  • Peer Reviewed
• [Journal Article] Differences in adipocytokines and fatty acid composition between two adipocyte fractions of small and large cells in high-fat diet-induced obese mice. (2009)
  • Author(s): Matsubara Y, Kano K, Kondo D, Mugishima H, Matsumoto T
  • Journal Title: Annals of Nutrition & Metabolism 54(4) Pages: 258-267
  • Peer Reviewed
• [Journal Article] Dedifferentiated fat cells convert to cardiomyocytes phenotype and repair infarcted cardiac tissue in rats. (2009)
  • Author(s): Jumabay M, Matsumoto T, Mugishima H, 他
  • Journal Title: Journal of Molecular and Cellular Cardiology 47(5) Pages: 565-575
  • Peer Reviewed
• [Journal Article] Nonhematopoietic stem cells in umbilical cord blood. (2009)
  • Author(s): Matsumoto T, Mugishima H
  • Journal Title: International Journal of Stem Cells 2(2) Pages: 83-89
  • Peer Reviewed
• [Journal Article] Dedifferentiated fat cells convert to cardiomyocytes phenotype and repair infarcted cardiac tissue in rats (2009)
  • Author(s): Jumabay M, Matsumoto T, Mugishima H, et al.
  • Journal Title: Journal of Molecular and Cellular Cardiology 47 Pages: 565-575
  • Peer Reviewed
• [Journal Article] Non-Hematopoietic stem cells in umbilical cord blood (2009)
  • Author(s): Matusmato T., Mugisima H.
  • Journal Title: International Journal of stem cells 2 Pages: 83-89
  • Peer Reviewed
• [Presentation] ヒト臍帯組織における間葉系幹細胞の局在および形質解析 (2011)
  • Author(s): 手塚里奈,松本太郎,石毛美夏,麦島秀雄, 他
  • Organizer: 第33回日本造血細胞移植療学会総会
  • Place of Presentation: 松山(ワークショップ)
  • Year and Date: 2011-03-10
• [Presentation] 骨髄破壊的前処置による骨髄造血微小環境への影響についての検討 (2011)
  • Author(s): 小高美奈子, 松本太郎, 石毛美夏, 辻孝, 麦島秀雄
  • Organizer: 第33回日本造血細胞移植学会総会
  • Place of Presentation: 松山
  • Year and Date: 2011-03-10
• [Presentation] 骨髄破壊的処置による骨髄造血微小環境への影響についての検討 (2011)
  • Author(s): 小高美奈子,麦島秀雄,松本太郎, 他
  • Organizer: 平成22年度日本大学学部連携研究推進シンポジウム
  • Place of Presentation: 日本大学幹細胞研究フォーラム,東京
  • Year and Date: 2011-01-22
• [Presentation] Dedifferentiated fat (DFAT) cells convert into cardiomyocytes phenotype and repair infarcted cardiac tissue in rats with myocardial infarction. (2010)
  • Author(s): Kazama T, Matsumoto T, Mugishima H, 他
  • Organizer: The 8th Annual Meeting of the International Federation for Adipose Therapeutics and Science (IFATS 2010)
  • Place of Presentation: Dallas, USA(Symposium)
  • Year and Date: 2010-10-22
• [Presentation] Dedifferentiated fat cells as a new cell source for regenerative medicine in patients with cardiovascular diseases. (2010)
  • Author(s): 松本太郎, 麦島秀雄, 他
  • Organizer: 第42回日本動脈硬化学会総会・学術集会
  • Place of Presentation: 岐阜(シンポジウム)
  • Year and Date: 2010-07-15
• [Presentation] 幹細胞に関する最近の話題と新規細胞治療法の開発 (2009)
  • Author(s): 松本太郎
  • Organizer: 第51回日本小児血液学会・第25回日本小児がん学会
  • Place of Presentation: 舞浜(ランチョンセミナー)
  • Year and Date: 2009-11-27
• [Presentation] 幹細胞に関する最近の話題と新規細胞治療法の開発 (2009)
  • Author(s): 松本太郎
  • Organizer: 第51回日本小児血液学会・第25回日本小児がん学会
  • Place of Presentation: 舞浜
  • Year and Date: 2009-11-27
• [Presentation] Dedifferentiated fat cells as a new cell source for regenerative medicine. (2009)
  • Author(s): Matsumoto T, Mugishima H, Wada M, 他
  • Organizer: The 9^<th> World Congress on Inflammation
  • Place of Presentation: Tokyo, Japan(Workshop)
  • Year and Date: 2009-07-08
• [Presentation] 脱分化脂肪細胞(DFAT)を用いた再生医療への取り組み (2008)
  • Author(s): 松本太郎
  • Organizer: 第10回記念循環器再生医療研究会
  • Place of Presentation: 東京(プレナリー)
  • Year and Date: 2008-11-29
• [Presentation] 脱分化脂肪細胞(DFAT)を用いた再生医療への取り組み (2008)
  • Author(s): 松本太郎, 他
  • Organizer: 第10回循環器再生医療研究会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-11-29
• [Book] 医学のあゆみ(脱分化脂肪細胞(DFAT)を細胞源とする再生医療 230(8)) (2009)
  • Author(s): 松本太郎,福田昇
  • Publisher: 医歯薬出版株式会社,東京
• [Book] 医学のあゆみ:脱分化脂肪細胞(DFAT)を細胞源とする再生医療 (2009)
  • Author(s): 松本太郎
  • Total Pages: 3
  • Publisher: 医歯薬出版株式会社