Exploration for novel murine hematopoietic stem cells by the use of intra-bone marrow injection methods
| Project/Area Number |
20591158
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
SASAKI Yutaka Kansai Medical University, 医学部, 講師 (80425066)
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| Co-Investigator(Renkei-kenkyūsha) |
SONODA Yoshiaki 関西医科大学, 医学部, 教授 (60206688)
HASE Makoto 関西医科大学, 医学部, 助教 (50425203)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 造血幹細胞 / 骨髄内移植 / c-kit / 移植・再生医療 |
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| Research Abstract |
Intra-bone marrow injection (IBMI) is a powerful method that enables highly efficient hematopoietic stem cell (HSC) engraftment in recipients' bone marrow. Using this method, novel HSCs that are not included in the c-kit^+Sca-1^+Lineage^-(KSL) compartment are explored. IBMI did not detect reproducibly the HSC activity in c-kit^-Sca^-1^+Linfraction, although in some rare cases, long-term hematopoietic reconstitution was observed in the fraction. Therefore, possibility remains that very rare HSCs exist in c-kit-fraction. On the other hand, c-kit^low fraction was proved to contain HSCs in low frequency. These HSCs were characterized.
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Report
(4 results)
Research Products
(11 results)
