Analysis of MHC restriction between hemopoietic stem cells and mesenchymal stem cells in humans and mice

• Project/Area Number: 20591159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hematology
• Research Institution: Kansai Medical University
• Principal Investigator: HISHA Hiriko Kansai Medical University, 医学部, 講師 (90151422)
• Co-Investigator(Kenkyū-buntansha): INABA Muneo 関西医科大学, 医学部, 准教授 (70115947)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 造血幹細胞 / 間葉系幹細胞 / MHC拘束性 / MHC class I分子 / 臍帯血 / 自己免疫疾患モデルマウス / 顆粒球・マクロファージコロニー刺激因子 / 神経細胞接着分子 / 接着分子 / 羊膜

Research Abstract

Engraftment of umbilical cord blood-derived hemopoietic stem cells (HSC) into SCID mice was facilitated when mesenchymal stem cells (MSCs) derived from the amnion of the same fetus were co-grafted. It is likely that G-CSF contributes to some extents to the preferential hemopoiesis-supporting ability of the MHC-matched MSCs. A higher proliferation of mouse HSCs was observed in co-culture system of HSCs expressing a high level of MHC class I molecule with MHC-matched MSCs than in that of HSCs expressing a low level of MHC class I molecule. Moreover, the abnormal proliferation (no MHC restriction) of HSCs in autoimmune-prone mice was attributed to increased expression of CD56 molecule on the HSC after the onset of autoimmune diseases.

Research Products

• [Journal Article] Mouse mesenchymal stem cells can support human hemopoiesis both in vitro and in vivo : Crucial role of neural cell adhesion molecule (NCAM). (2010)
  • Author(s): Xiaoli Wang, Hiroko Hisha, Tomomi Mizokami, Wenhao Cui, Yunze Cui, Aiping Shi, Changye Song, Satoshi Okazaki, Qing Li, Wei Feng, Junko Kato, Susumu Ikehara
  • Journal Title: Haematologica 95 Pages: 884-91
  • Peer Reviewed
• [Journal Article] Bone marrow-derived stromal cells can express neuronal markers by DHA/GPR40 signaling. (2010)
  • Author(s): Desislav B. Kaplamadzhiev, Hiroko Hisha, Yasushi Adachi, Susumu Ikehara, Anton B. Tonchev, Nadezhda B. Boneva, Ilia V. Pyko, Mitsuru Kikuchi, Masa-aki Nakaya, Tomohoro Wakayama, Shoichi Isaki, Tetsumori Yamashima
  • Journal Title: Bioscience Trends 4 Pages: 119-129
  • Peer Reviewed
• [Journal Article] Bone marrow-derived stromal cells can express neuronal markers by DHA/GPR40 signaling (2010)
  • Author(s): Desislav B.Kaplamadzhiev
  • Journal Title: Bioscience Trends Volume: 4 Pages: 119-129
  • Peer Reviewed
• [Journal Article] Mouse mesenchymal stem cells can support human hemopoiesis both in vitro and in vivo : crucial role of neural cell adhesion molecule (NCAM) (2010)
  • Author(s): Xiaoli Wang
  • Journal Title: Haematologica Volume: 95 Pages: 884-891
  • Peer Reviewed
• [Journal Article] Transformation potential of bone marrow stromal cells into undifferentiated high grade pleomorphic sarcoma (2010)
  • Author(s): Qing Li
  • Journal Title: J Cancer Res and Clin Oncol Volume: 136 Pages: 826-838
  • Peer Reviewed
• [Journal Article] Preferential expansion of human umbilical cord blood-derived CD34-positive cells on MHC-matched amnion-derived mesenchymal stem cells. (2009)
  • Author(s): Tomomi Mizokami, Hiroko Hisha, Satoshi Okazaki, Takashi Takaki, Xiao-Li Wang, Chang-Ye Song, Qing Li, Junko Kato, Naoki Hosaka, Muneo Inaba, Hideharu Kanzaki, Susumu Ikehara
  • Journal Title: Haematologica 94 Pages: 618-628
  • Peer Reviewed
• [Journal Article] Preferential expansion of human umbilical cord blood-derived CD34-positive cells on MHC-matched amnion-derived mesenchymal stem cells (2009)
  • Author(s): Tomomi Mizokami
  • Journal Title: Haematologica 94 Pages: 618-628
  • Peer Reviewed
• [Journal Article] Mouse mesenchymal stem cells can support human hemopoiesis both in vitro and in vivo : crucial role of neural cell adhesion molecule(NCAM) (2009)
  • Author(s): Xiaoli Wang
  • Journal Title: Haematologica
  • Peer Reviewed
• [Journal Article] Transformation potential of bone marrow stromal cells into undifferentiated high grade pleomorphic sarcoma (2009)
  • Author(s): Qing Li
  • Journal Title: J Cancer Res and Clin Oncol
  • Peer Reviewed
• [Journal Article] Contribution of neural cell adhesion molecule (NCAM) to hemopoietic system in monkeys. (2008)
  • Author(s): Junko Kato, Hiroko Hisha, Xiaoli Wang, Tomomi Mizokami, Satoshi Okazaki, Qing Li, Changye Song, Masahiko Maki, Naoki Hosaka, Yasushi Adachi, Muneo Inaba, Susumu Ikehara
  • Journal Title: Ann Hematol 87 Pages: 797-807
  • Peer Reviewed
• [Journal Article] Facilitation of hematopoietic recovery by bone grafts with intra-bone marrow-bone marrow transplantation. (2008)
  • Author(s): Changye Song, Hiroko Hisha, Xiaoli Wang, Qing Li, Ming Li, Wenhao Cui, Kequan Guo, Satoshi Okazaki, Tomomi Mizokami, Junko Kato, Yunze Cui, Wei Feng, Yuming Zhang, Ming Shi, Muneo Inaba, Hongxue Fan, Susumu Ikehara
  • Journal Title: Immunobiology 213 Pages: 455-468
  • Peer Reviewed
• [Journal Article] Facilitation of hemopoietic recovery by bone grafts with intra-bone marrow-bone marrow transplantation (2008)
  • Author(s): Changye Song
  • Journal Title: Immunobiology 213 Pages: 455-468
  • Peer Reviewed
• [Journal Article] Contribution of neural cell adhesion molecule (NCAM) To hemopoietic system in monkeys (2008)
  • Author(s): Junko Kato
  • Journal Title: Annals of Hematology 87 Pages: 797-807
  • Peer Reviewed
• [Journal Article] Successful acceptance of adult liver allograft by intra-bone marrow-bone marrow transplantation (2008)
  • Author(s): Satoshi Okazaki
  • Journal Title: Stem Cells and Development 17 Pages: 629-639
  • Peer Reviewed
• [Presentation] Transformation potential of bone marrow stromal cells into undifferentiated high grade pleomorphic sarcoma (2010)
  • Author(s): Qing Li
  • Organizer: The 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場
  • Year and Date: 2010-08-25
• [Presentation] Hemopoiesis-supporting capacity of mouse mesenchymal stem cell line : Crucial role of neural cell adhesion molecule (NCAM). (2010)
  • Author(s): Hiroko Hisha, Xiaoli Wang, Junko Kato, Tomomi Mizokami, Qing Li, Hideharu Kanzaki, Susumu Ikehara
  • Organizer: The 14th International Congress of Immunology
  • Place of Presentation: 日神戸(神戸ポートピアホ テル、神戸国際展示場)
  • Year and Date: 2010-08-23
• [Presentation] Human hemopoiesis-supporting capacity of mouse mesenchymal stem cell line : crucial role of neural cell adhesion molecule (NCAM) (2010)
  • Author(s): Hiroko Hisha
  • Organizer: The 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸ポートピアホテル・神戸国際展示場
  • Year and Date: 2010-08-23
• [Presentation] Characterization of neural cell adhesion molecule (NCAM) on mouse, monkey and human mesenchymal stem cells. (2009)
  • Author(s): Junko Kato, Hiroko Hisha, Qing Li, Yasushi Adachi, Muneo Inaba, Naoki Hosaka, Masahiko Maki, Susumu Ikehara
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪(大阪国際会議場)
  • Year and Date: 2009-12-02
• [Presentation] Repeated infusions of donor blood, BMCs and PBSCs can restore early hematopoiesis after allogenic BMT (2009)
  • Author(s): 李清
  • Organizer: 第39回 日本免疫学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] Characterization of neural cell adhesion molecule(NCAM)on mouse monkey and human mesenchymal stem cells (2009)
  • Author(s): 加藤順子
  • Organizer: 第39回 日本免疫学会総会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] 神経細胞接着分子(NCAM、CD56)のサルおよびヒト造血系への関与 (2009)
  • Author(s): 加藤順子、比舍弘子、李清、池原進
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 京都(国立京都国際会館)
  • Year and Date: 2009-05-01
• [Presentation] Repeated infusions of donor BMCs and PBSCs can restore early hematopoiesis after allogenic BMT (2009)
  • Author(s): 李清
  • Organizer: 第98回日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-05-01
• [Presentation] 神経細胞接着分子(NCAM、CD56)のサルおよびヒト造血系への関与 (2009)
  • Author(s): 加藤順子
  • Organizer: 第98回 日本病理学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2009-05-01
• [Presentation] Contribution of neural cell adhesion molecule (NCAM) to monkey hemopoietic systems (III) (2008)
  • Author(s): 加藤順子
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Infusion of donor BMCs and blood improves low leukocyte counts in early phase after allogenic BMT, resulting in prevention of infection (2008)
  • Author(s): 李清
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Facilitating effects of intra-bone marrow-bone marrow transplantation (IBM-BMT) co-grafted with donor bones on hemopoietic recovery. (2008)
  • Author(s): Changye Song, Hiroko Hisha, Qing Li, Wenhao Cui, Kequan Guo, Wei Feng, Junko Kato, Susumu Ikehara
  • Organizer: 第97回日本病理学会総会
  • Place of Presentation: 金沢(石川県立音楽堂、ホテル日航金沢、もてなしドーム地下広場)
  • Year and Date: 2008-05-17
• [Presentation] Facilitating effects of IBM-BMT co-grafted with donor bones on hematopoietic recovery (2008)
  • Author(s): 宋昌嘩
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] Development of malignant fibrous histiocytoma from mouse mesenchymal cells (2008)
  • Author(s): 李清
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-17
• [Presentation] サル骨髄由来間葉系幹細胞における神経細胞接着分子(NCAM)の発現とその造血系への関与 (2008)
  • Author(s): 加藤順子
  • Organizer: 第97回日本病理学会
  • Place of Presentation: 金沢
  • Year and Date: 2008-05-16
• [Book] Adhesion Molecules (Edited by Preedy VR) (2010)
  • Author(s): Hiroko Hisha, Xiaoli Wang, Susumu Ikehara
  • Publisher: Scientific Publishers
• [Book] Adhesion molecule (2010)
  • Author(s): Hiroko Hisha
  • Total Pages: 524
  • Publisher: Scientific Puhlishers