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Cloning and analysis of molecules involved in functional regulation of β3 integrin

Research Project

Project/Area Number 20591162
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

HONDA Shigenori  National Cardiovascular Center Research Institute, 分子病態部, 室長 (00303959)

Co-Investigator(Kenkyū-buntansha) TOMIYAMA Yoshiaki  大阪大学, 医学部附属病院, 教授 (80252667)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsβ3インテグリン / シグナル伝達 / ILK / クローニング
Research Abstract

(1) We clarified that ILK is important for functional expression of β3 integrin and this process is necessary for the full-length of ILK but not each ILK-domain fragment. It was suggested that the kinase activity of ILK is not essential for integrin activation. In addition, we showed that ILK forms a complex with an intracellular adaptor protein, PINCH and Parvin, and this complex formation might be needed to stabilize each protein.
(2)We established mutant cell lines with inactivated integrin αIIbα6Bβ3 to which is converted by a treatment of chemical mutagen. Transfection of ILK cDNA into the mutant cells did not induce integrin activation, suggesting that those mutant cells might have defects in molecules except for ILK, which resulted in the inactivated integrin state. We are performing expression cloning to isolate molecules involved in functional expression of β3 integrin.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (19 results)

All 2011 2010 2009 2008 Other

All Journal Article (12 results) (of which Peer Reviewed: 5 results) Presentation (5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] A potential role for α-actinin in inside-out αIIbβ3 signaling2011

    • Author(s)
      Tadokoro S, et al.
    • Journal Title

      Blood

      Volume: 117 Pages: 250-258

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Biochem Biophys2010

    • Author(s)
      T.Ohmori, Y.Kashiwakura, A.Ishiwata, S.Madoiwa, J.Mimuro, S.Honda, T.Miyata, Y.Sakata.
    • Journal Title

      Res Commun 400

      Pages: 323-328

    • Related Report
      2010 Final Research Report
  • [Journal Article] 「キンドリンとインテグリン活性化」Annual Review 血液2010(高久史麿・小澤敬也・坂田洋一・金倉譲・小島勢二編集)2010

    • Author(s)
      冨山佳昭、本田繁則
    • Journal Title

      中外医学社

      Pages: 160-167

    • Related Report
      2010 Final Research Report
  • [Journal Article] Vinculin activates inside-out signaling of integrin αIIbβ3 in Chinese hamster ovary cells2010

    • Author(s)
      Ohmori T, et al
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 400 Pages: 323-328

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integrin-linked kinase associated with integrin activation.2009

    • Author(s)
      S.Honda, Shirotani-Ikejima H, S.Tadokoro, Y.Maeda, T.Kinoshita, Y.Tomiyama, T.Miyata
    • Journal Title

      Blood 113

      Pages: 5304-5313

    • Related Report
      2010 Final Research Report
  • [Journal Article] Blood Coagul2009

    • Author(s)
      J.Ishikawa, H.Okada, H.Kato, S.Takeshita, S.Honda, T.Kawasaki, E.Suehisa, H.Tsuji, S.Madoiwa, Y.Sakata, T.Kojima, M.Murata, Y.Ikeda, Y.Kokubo, T.Okamura, H.Tomoike, T.Miyata
    • Journal Title

      Fibrinolysis 20

      Pages: 22-26

    • Related Report
      2010 Final Research Report
  • [Journal Article] 変異導入法を用いた血小板インテグリンαIIbβ3の機能調節に関わる分子の探索2009

    • Author(s)
      本田繁則、池島-城谷裕子、松田泰幸、田所誠司、前田祐輔、木下タロウ、冨山佳昭、宮田敏行
    • Journal Title

      日本血栓止血学会誌 20(6)

      Pages: 615-621

    • NAID

      10026118398

    • Related Report
      2010 Final Research Report
  • [Journal Article] Integrin-linked kinase associated with integrin activation2009

    • Author(s)
      S.HONDA, et al.
    • Journal Title

      Blood VOL.113

      Pages: 5304-5313

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 変異導入法を用いた血小板インテグリンαIIbβ3の機能調節に関わる分子の探索2009

    • Author(s)
      本田繁則, 他
    • Journal Title

      日本血栓止血学会誌 20巻

      Pages: 615-621

    • NAID

      10026118398

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Integrin-linked kinase associated with integrin activation2009

    • Author(s)
      S. HONDA
    • Journal Title

      Blood (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence of genetic mutations in protein S, protein C and antithrombin genes in Japanese patients with deep vein thrombosis2009

    • Author(s)
      T. Miyata
    • Journal Title

      Thromb Res 124

      Pages: 14-18

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article]2008

    • Author(s)
      T.Miyata, Y.Sato, J.Ishikawa, H.Okada, S.Takeshita, T.Sakata, K.Kokame, R.Kimura, S.Honda, T.Kawasaki, E.Suehisa, H.Tsuji, S.Madoiwa, Y.Sakata, T.Kojima, M.Murata, Y.Ikeda.
    • Journal Title

      Thromb Res 124

      Pages: 14-18

    • Related Report
      2010 Final Research Report
  • [Presentation] インテグリン活性化におけるintegrin-linked kinaseの役割2010

    • Author(s)
      本田繁則、池島裕子、松田泰幸、田所誠司、冨山佳昭、宮田敏行
    • Organizer
      第72回日本血液学会総会
    • Place of Presentation
      横浜市
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] XXII Congress of The International Society on Thrombosis and Haemostasis2009

    • Author(s)
      本田繁則, 池島-城谷裕子, 田所誠司, 前田祐輔, 木下タロウ, 冨山佳昭, 宮田敏行
    • Organizer
      Integrin-linked kinase associated with integrin activation
    • Place of Presentation
      Boston Convention & Exhibition Center (MA USA)
    • Year and Date
      2009-07-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Integrin-linked kinase associated with integrin activation2009

    • Author(s)
      S.HONDA
    • Organizer
      XXII Congress of The International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Boston Convention & Exhibition Center(MA USA)
    • Year and Date
      2009-07-16
    • Related Report
      2009 Annual Research Report
  • [Presentation] 変異導入法を用いたインテグリン機能発現分子の同定2008

    • Author(s)
      本田繁則、池島-城谷裕子、田所誠司、前田祐輔、木下タロウ、冨山佳昭、宮田敏行
    • Organizer
      第70回日本血液学会総会
    • Place of Presentation
      京都市
    • Related Report
      2010 Final Research Report
  • [Presentation] 発現クローニングによるインテグリン機能発現分子の同定2008

    • Author(s)
      本田繁則
    • Organizer
      第31回日本血栓止血学会学術集会SPCシンポジウム
    • Place of Presentation
      大阪国際交流センター
    • Related Report
      2008 Annual Research Report
  • [Book] Annual Review血液20102010

    • Author(s)
      冨山佳昭、本田繁則
    • Total Pages
      8
    • Publisher
      中外医薬社
    • Related Report
      2009 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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