Cloning and analysis of molecules involved in functional regulation of β3 integrin
| Project/Area Number |
20591162
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | National Cardiovascular Center Research Institute |
|---|
Principal Investigator |
HONDA Shigenori National Cardiovascular Center Research Institute, 分子病態部, 室長 (00303959)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
TOMIYAMA Yoshiaki 大阪大学, 医学部附属病院, 教授 (80252667)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | β3インテグリン / シグナル伝達 / ILK / クローニング |
|---|
| Research Abstract |
(1) We clarified that ILK is important for functional expression of β3 integrin and this process is necessary for the full-length of ILK but not each ILK-domain fragment. It was suggested that the kinase activity of ILK is not essential for integrin activation. In addition, we showed that ILK forms a complex with an intracellular adaptor protein, PINCH and Parvin, and this complex formation might be needed to stabilize each protein.
(2)We established mutant cell lines with inactivated integrin αIIbα6Bβ3 to which is converted by a treatment of chemical mutagen. Transfection of ILK cDNA into the mutant cells did not induce integrin activation, suggesting that those mutant cells might have defects in molecules except for ILK, which resulted in the inactivated integrin state. We are performing expression cloning to isolate molecules involved in functional expression of β3 integrin.
|
Report
(4 results)
Research Products
(19 results)
-
-
[Journal Article] Biochem Biophys2010
Author(s)
T.Ohmori, Y.Kashiwakura, A.Ishiwata, S.Madoiwa, J.Mimuro, S.Honda, T.Miyata, Y.Sakata.
-
Journal Title
Res Commun 400
Pages: 323-328
Related Report
-
-
-
-
[Journal Article] Blood Coagul2009
Author(s)
J.Ishikawa, H.Okada, H.Kato, S.Takeshita, S.Honda, T.Kawasaki, E.Suehisa, H.Tsuji, S.Madoiwa, Y.Sakata, T.Kojima, M.Murata, Y.Ikeda, Y.Kokubo, T.Okamura, H.Tomoike, T.Miyata
-
Journal Title
Fibrinolysis 20
Pages: 22-26
Related Report
-
-
-
-
-
-
[Journal Article]2008
Author(s)
T.Miyata, Y.Sato, J.Ishikawa, H.Okada, S.Takeshita, T.Sakata, K.Kokame, R.Kimura, S.Honda, T.Kawasaki, E.Suehisa, H.Tsuji, S.Madoiwa, Y.Sakata, T.Kojima, M.Murata, Y.Ikeda.
-
Journal Title
Thromb Res 124
Pages: 14-18
Related Report
-
-
-
-
-
-
-
