| Project/Area Number |
20591179
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | University of Occupational and Environmental Health, Japan |
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Principal Investigator |
SAITO Kazuyoshi University of Occupational and Environmental Health, Japan, 医学部, 准教授 (30279327)
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| Co-Investigator(Kenkyū-buntansha) |
KOHNO Kimitoshi 産業医科大学, 医学部, 教授 (00153479)
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| Co-Investigator(Renkei-kenkyūsha) |
TANAKA Yoshiya 産業医科大学, 医学部, 教授 (30248562)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 膠原病学 / 抗転写因子抗体 / 膠原病 / 転写因子 / 自己抗体 |
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| Research Abstract |
We developed a new detection system for anti-body against transcriptional factors in connective tissue diseases. Ninety six nuclear factors that involved in cell cycle and homeostasis were blotted on the DEAE paper and incubated with serum from the patients with connective tissue diseases including SLE, dermatomyositis, polyangitis. Anti-HMG1 antibody was detected in 2 patients with polyangitis. Antibody against PCNA was observed in 2 SLE but the serum reacted with a lot of nuclear factors irrelevant with organ specificity. Acetylation and methylation of nuclear factors did not affect on the result of detection of auto-antibody.
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