| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Human umbilical cord blood-derived mononuclear cells were cultured in the presence of 10 ng/ml IL-3 for 2 weeks. Alcian blue stained cells were isolated after the culture. These premature basophils were passively sensitized with human IgE and stimulated by anti-human IgE polyclonal antibody (1 μg/ml). Substantial amount of IL-8 was detected in cell supernatants at 3h after the stimulation. Although the expression of IgE Fc receptors in these cells was analyzed by flowcytometry, the expression was a little. To increase the expression of IgE Fc receptors, CD34+ cells isolated from human cord blood were cultured in the presence of 500 ng/ml IgE as well as 10 ng/ml IL-3 for 2 weeks. Substantial expression of IgE Fc receprors was observed on cell surface of CD34+ cell-derived premature basophils cultured in this condition. IL-4, IL-8, and leukotriene C4 were detected in the supernatants of CD34+ cell-derived premature basophils following the stimulation of anti-IgE.
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