Comprehensive analysis of eosinophil-specific mRNA expression profiles

• Project/Area Number: 20591196
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: National Research Institute for Child Health and Development
• Principal Investigator: MATSUMOTO Kenji National Research Institute for Child Health and Development, 免疫アレルギー研究部・アレルギー研究室, 室長 (60181765)
• Research Collaborator: SAITO Hirohisa 独立行政法人国立成育医療研究センター, 免疫アレルギー研究部, 部長 (40130166)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 好酸球 / マイクロアレイ / 網羅的遺伝子発現解析 / Amphyregulin / CCL23 / サイトカイン・ケモカイン / Amphiregulin

Research Abstract

The number of eosinophils is well known to be one of the best clinical markers for assessing the severity of allergic inflammation; however, the precise roles of eosinophils in vivo remain unclear. In the present study we comprehensively analyzed mRNA expression profiles of eosinophils after exposure to various cytokines, chemokines and immobilized immunoglobulins, and compared them with those of human blood cells. As a result, we identified a number of genes specifically expressed in human eosinophil. Those included cytokines and chemokines, suggesting that eosinophils may play important roles not only as effecter cells but also as regulatory cells in vivo.

Research Products

• [Journal Article] Eosinophilic gastrointestinal disorders in infants : a Japanese case series. (2011)
  • Author(s): Yamada Y, Nishi A, Ebara Y, Kato M, Yamamoto H, Morita H, Nomura I, Matsumoto K, Hirato J, Hatakeyama SI, Suzuki N, Hayashi Y.
  • Journal Title: Int Arch Allergy Immunol. 155 Suppl 1 Pages: 40-5
• [Journal Article] Paracrine IL-33 stimulation enhances lipopolysaccharide-mediated macrophage activation. (2011)
  • Author(s): Ohno T, Oboki K, Morita H, Kajiwara N, Arae K, Tanaka S, Ikeda M, Iikura M, Akiyama T, Inoue J, Matsumoto K, Sudo K, Azuma M, Okumura K, Kamradt T, Saito H, Nakae S.
  • Journal Title: PLoS ONE. 6
• [Journal Article] IL-33 and Airway Inflammation. (2011)
  • Author(s): Oboki K, Nakae S, Matsumoto K, Saito H.
  • Journal Title: Allergy Asthma Immunol Res. 3 Pages: 81-8
• [Journal Article] Four distinct subtypes of non-IgE-mediated gastro- intestinal food allergies in neonates and infants, distinguished by their initial symptoms. (2011)
  • Author(s): Nomura I, Morita H, Hosokawa S, Hoshina H, Fukuie T, Watanabe M, Ohtsuka Y, Shoda T, Terada A, Takamasu T, Arai K, Ito Y, Ohya Y, Saito H, Matsumoto K.
  • Journal Title: J Allergy Clin Immunol. 127 Pages: 685-8
• [Journal Article] Human eosinophils produce and release a novel chemokine, CCL23, in vitro. (2011)
  • Author(s): Matsumoto K, Fukuda S, Hashimoto N, Saito H.
  • Journal Title: Int Arch Allergy Immunol. 155 Suppl 1 Pages: 34-9
• [Journal Article] Peripheral blood mononuclear cells from patients with bronchial asthma show impaired innate immune responses to rhinovirus in vitro. (2011)
  • Author(s): Iikura K, Katsunuma T, Saika S, Saito S, Ichinohe S, Ida H, Saito H, Matsumoto K.
  • Journal Title: Int Arch Allergy Immunol. 155 Suppl 1 Pages: 27-33
• [Journal Article] Thymic stromal lymphopoietin gene promoter polymorphisms are associated with susceptibility to bronchial asthma. (2011)
  • Author(s): Harada M, Hirota T, Jodo AI, Hitomi Y, Sakashita M, Tsunoda T, Miyagawa T, Doi S, Kameda M, Fujita K, Miyatake A, Enomoto T, Noguchi E, Masuko H, Sakamoto T, Hizawa N, Suzuki Y, Yoshihara S, Adachi M, Ebisawa M, Saito H, Matsumoto K, Nakajima T, Mathias RA, Rafaels N, Barnes KC, Himes BE, Duan QL, Tantisira KG, Weiss ST, Nakamura Y, Ziegler SF, Tamari M.
  • Journal Title: Am J Respir Cell Mol Biol. 44 Pages: 787-93
• [Journal Article] Human eosinophils produce and release a novel chemokine, CCL23, in vitro. (2011)
  • Author(s): Kenji Matsumoto
  • Journal Title: International Archives of Allergy and Immunology Volume: 155 suppl 1 Pages: 34-39
  • Peer Reviewed
• [Journal Article] Eosinophilic gastrointestinal disorders (EGIDs) in infants-A Japanese case series. (2011)
  • Author(s): Yoshiyuki Yamada
  • Journal Title: International Archives of Allergy and Immunology Volume: 155 suppl 1 In Press
  • Peer Reviewed
• [Journal Article] Effect of Th1/Th2 Cytokine Pretreatment on RSV-Induced Gene Expression in Airway Epithelial Cells. (2010)
  • Author(s): Yamada Y, Matsumoto K, Hashimoto N, Saikusa M, Homma T, Yoshihara S, Saito H.
  • Journal Title: Int Arch Allergy Immunol. 154 Pages: 185-94
• [Journal Article] IL-33 mediates inflammatory responses in human lung tissue cells. (2010)
  • Author(s): Yagami A, Orihara K, Morita H, Futamura K, Hashimoto N, Matsumoto K, Saito H, Matsuda A.
  • Journal Title: J Immunol. 185 Pages: 5743-50
• [Journal Article] IQGAP1 and vimentin are key regulator genes in naturally occurring hepatotumorigenesis induced by oxidative stress. (2010)
  • Author(s): Tsubota A, Matsumoto K, Mogushi K, Nariai K, Namiki Y, Hoshina S, Hano H, Tanaka H, Saito H, Tada N.
  • Journal Title: Carcinogenesis. 31 Pages: 504-11
• [Journal Article] Defining cell identity by comprehensive gene expression profiling. (2010)
  • Author(s): Toyoda M, Hamatani T, Okada H, Matsumoto K, Saito H, Umezawa A.
  • Journal Title: Curr Med Chem. 17 Pages: 3245-52
• [Journal Article] Overexpression of LEDGF/DFS70 induces IL-6 via p38 activation in HaCaT cells, similar to that seen in the psoriatic condition. (2010)
  • Author(s): Takeichi T, Sugiura K, Muro Y, Matsumoto K, Ogawa Y, Futamura K, Kaminuma O, Hashimoto N, Shimoyama Y, Saito H, Tomita Y.
  • Journal Title: J Invest Dermatol. 130 Pages: 2760-7
• [Journal Article] IL-33 is a crucial amplifier of innate rather than acquired immunity. (2010)
  • Author(s): Oboki K, Ohno T, Kajiwara N, Arae K, Morita H, Ishii A, Nambu A, Abe T, Kiyonari H, Matsumoto K, Sudo K, Okumura K, Saito H, Nakae S.
  • Journal Title: Proc Natl Acad Sci USA. 107 Pages: 18581-6
• [Journal Article] Intradiscal transplantation of synovial mesenchymal stem cells prevents inter- vertebral disc degeneration through supper- ssion of matrix metalloproteinase-related genes in nucleus pulposus cells in rabbits. (2010)
  • Author(s): Miyamoto T, Muneta T, Tabuchi T, Matsumoto K, Saito H, Tsuji K, Sekiya I.
  • Journal Title: Arthritis Res Ther. 12
• [Journal Article] Analysis of signal transduction pathways involved in anti-CD30 mAb-induced human eosinophil apoptosis. (2010)
  • Author(s): Matsumoto K, Terakawa M, Fukuda S, Saito H.
  • Journal Title: Int Arch Allergy Immunol. 152 Suppl 1 Pages: 2-8
• [Journal Article] Cells of extraembryonic mesodermal origin confer human dystrophin in the Mdx model of duchenne muscular dystrophy. (2010)
  • Author(s): Kawamichi Y, Cui CH, Toyoda M, Makino H, Horie A, Takahashi Y, Matsumoto K, Saito H, Ohta H, Saito K, Umezawa A.
  • Journal Title: J Cell Physiol. 223 Pages: 695-702
• [Journal Article] beta(2)-adrenoceptor agonists enhance cytokine-induced release of thymic stromal lymphopoietin by lung tissue cells. (2010)
  • Author(s): Futamura K, Orihara K, Hashimoto N, Morita H, Fukuda S, Sagara H, Matsumoto K, Tomita Y, Saito H, Matsuda A.
  • Journal Title: Int Arch Allergy Immunol. 152 Pages: 353-61
• [Journal Article] Proactive treatment appears to decrease serum immunoglobulin-E levels in patients with severe atopic dermatitis. (2010)
  • Author(s): Fukuie T, Nomura I, Horimukai K, Manki A, Masuko I, Futamura M, Narita M, Ohzeki T, Matsumoto K, Saito H, Ohya Y.
  • Journal Title: Br J Dermatol. 163 Pages: 1127-9
• [Journal Article] Identification of a polyI:C-inducible membrane protein that participates in dendritic cell-mediated natural killer cell activation. (2010)
  • Author(s): Ebihara T, Azuma M, Oshiumi H, Kasamatsu J, Iwabuchi K, Matsumoto K, Saito H, Taniguchi T, Matsumoto M, Seya T.
  • Journal Title: J Exp Med. 207 Pages: 2675-87
  • NAID: 120002722561
• [Journal Article] Analysis of signal transduction pathways involved in anti-CD30 mAb-induced human eosinophil apoptosis (2010)
  • Author(s): Kenji Matsumoto
  • Journal Title: International Archives of Allergy and Immunology Volume: 152 suppl 1 Pages: 2-8
  • Peer Reviewed
• [Journal Article] Analysis of signal transduction pathways involved in anti-CD30 mAb-induced human eosinophil apoptosis (2010)
  • Author(s): Kenji Matsumoto
  • Journal Title: International Archives of Allergy and Immunology 152 suppl 1 Pages: 2-8
  • Peer Reviewed
• [Journal Article] Lactobacillus rhamnosus GG and Lactobacillus casei suppress Escherichia coli-Induced chemokine expression in intestinal epithelial cells. (2009)
  • Author(s): Toki S, Kagaya S, Shinohara M, Wakiguchi H, Matsumoto T, Takahata Y, Morimatsu F, Saito H, Matsumoto K.
  • Journal Title: Int Arch Allergy Immunol. 148 Pages: 45-58
• [Journal Article] Impaired CD4 and CD8 effector function and decreased memory T cell populations in ICOS-deficient patients. (2009)
  • Author(s): Takahashi N, Matsumoto K, Saito H, Nanki T, Miyasaka N, Kobata T, Azuma M, Lee SK, Mizutani S, Morio T.
  • Journal Title: J Immunol. 182 Pages: 5515-27
• [Journal Article] The unfolded protein response is activated in differentiating epidermal keratinocytes. (2009)
  • Author(s): Sugiura K, Muro Y, Futamura K, Matsumoto K, Hashimoto N, Nishizawa Y, Nagasaka T, Saito H, Tomita Y, Usukura J.
  • Journal Title: J Invest Dermatol. 129 Pages: 2126-35
• [Journal Article] TH2 cytokines potently induce an appetite-stimulating peptide, melanin-concentrating hormone, in human vascular endothelial cells. (2009)
  • Author(s): Orihara K, Morita H, Yagami A, Kajiwara N, Nakae S, Matsumoto K, Nagasaki H, Saito Y, Saito H, Matsuda A.
  • Journal Title: J Allergy Clin Immunol. 124
• [Journal Article] Effect of heat treatment and enzymatic digestion on the B cell epitopes of cow's milk proteins. (2009)
  • Author(s): Morisawa Y, Kitamura A, Ujihara T, Zushi N, Kuzume K, Shimanouchi Y, Tamura S, Wakiguchi H, Saito H, Matsumoto K.
  • Journal Title: Clin Exp Allergy. 39 Pages: 918-25
• [Journal Article] Amphiregulin production by human eosinophils. (2009)
  • Author(s): Matsumoto K, Fukuda S, Nakamura Y, Saito H.
  • Journal Title: Int Arch Allergy Immunol. 149 Suppl 1 Pages: 39-44
• [Journal Article] Dexamethasone and FK506 inhibit expression of distinct subsets of chemokines in human mast cells. (2009)
  • Author(s): Kato A, Chustz RT, Ogasawara T, Kulka M, Saito H, Schleimer RP, Matsumoto K.
  • Journal Title: J Immunol. 182 Pages: 7233-43
• [Journal Article] Large scale genotyping study for asthma in the Japanese population. (2009)
  • Author(s): Imada Y, Fujimoto M, Hirata K, Hirota T, Suzuki Y, Saito H, Matsumoto K, Akazawa A, Katsunuma T, Yoshihara S, Ebisawa M, Shibasaki M, Arinami T, Tamari M, Noguchi E.
  • Journal Title: BMC Res Notes. 2 Pages: 54-54
• [Journal Article] Functional analysis of the thymic stromal lymphopoietin variants in human bronchial epithelial cells. (2009)
  • Author(s): Harada M, Hirota T, Jodo AI, Doi S, Kameda M, Fujita K, Miyatake A, Enomoto T, Noguchi E, Yoshihara S, Ebisawa M, Saito H, Matsumoto K, Nakamura Y, Ziegler SF, Tamari M.
  • Journal Title: Am J Respir Cell Mol Biol. 40 Pages: 368-74
• [Journal Article] Advances in mechanisms of asthma, allergy, and immunology in 2008. (2009)
  • Author(s): Boyce JA, Broide D, Matsumoto K, Bochner BS.
  • Journal Title: J Allergy Clin Immunol. 123 Pages: 569-74
• [Journal Article] Advances in mechanisms of asthma, allergy, and immunology in 2008 (2009)
  • Author(s): Joshua A Boyce
  • Journal Title: Journal of Allergy and Clinical Immunology 123 Pages: 569-574
  • Peer Reviewed
• [Journal Article] Amphiregulin production by human eosinophils (2009)
  • Author(s): Kenji Matsumoto
  • Journal Title: International Archives of Allergy and Immunology 149 suppl 1 Pages: 39-44
  • Peer Reviewed
• [Journal Article] Interleukin-33 enhances adhesion, CD11b expression and survival in human eosinophils (2009)
  • Author(s): Maho Suzukawa
  • Journal Title: Laboratory Investigation 88 Pages: 1245-1253
  • Peer Reviewed
• [Journal Article] Amphiregulin production by human eosinophil (2009)
  • Author(s): Kenji Matsumoto, Shuhei Fukuda, Yuri Nakamura, Hirohisa Saito
  • Journal Title: International Archives of Allergy and Immunology 149(suppl 1) Pages: 39-44
  • Peer Reviewed
• [Journal Article] Presence of eosinophils in nasal secretion during acute respiratory tract Infection in young children predicts subsequent wheezing within two months. (2008)
  • Author(s): Shinohara M, Wakiguchi H, Saito H, Matsumoto K.
  • Journal Title: Allergol Int. 57 Pages: 359-65
  • NAID: 10025108490
• [Journal Article] Involvement of eosinophils in the onset of asthma (2008)
  • Author(s): Kenji Matsumoto, Mayumi Tamari, Hirohisa Saito
  • Journal Title: Journal of Allergy and Clinical Immunology 121 Pages: 26-27
  • Peer Reviewed
• [Journal Article] Presence of Eosinophils in Nasal Secretion during Acute Respiratory Tract Infection in Young Children Predicts Subsequent Wheezing within Two Months (2008)
  • Author(s): Miwa Shinohara, Hiroshi Wakiguchi, Hirohisa Saito, Kenji Matsumoto
  • Journal Title: Allergology International 57 Pages: 359-365
  • NAID: 10025108490
  • Peer Reviewed
• [Presentation] 好酸球特異的な機能の網羅的探索 (2010)
  • Author(s): 松本健治
  • Organizer: 第60回日本アレルギー学会総会
  • Place of Presentation: 東京
• [Presentation] CCL23 production by human eosinophils (2010)
  • Author(s): Matsumoto K, Fukuda S, Saito H.
  • Organizer: 28th Symposium of the Collegium Internationale Allergologicum
  • Place of Presentation: Ischia, Italy
• [Presentation] Analysis of signal transduction pathways involved in anti-CD30 mAb-induced human eosinophil apoptosis (2010)
  • Author(s): Matsumoto K, Terakawa M, Fukuda S, Saito H.
  • Organizer: 66th Annual Meeting of American Academy of Allergy, Asthma & Immunology
  • Place of Presentation: New Orleans, USA
• [Presentation] Induction of apoptosis in human eosinophils (2009)
  • Author(s): 松本健治
  • Organizer: 第59回日本アレルギー学会秋季学術大会 International symposium
  • Place of Presentation: 秋田
• [Presentation] Production of amphiregulin by human eosinophils (2009)
  • Author(s): Matsumoto K, Fukuda S, Saito H.
  • Organizer: 65th Annual Meeting of American Academy of Allergy, Asthma & Immunology
  • Place of Presentation: Washington DC, USA
• [Presentation] Production of Amphiregulin by Human Eosinophils (2009)
  • Author(s): Kenji Matsumoto, Shuhei Fukuda, Hirohisa Saito
  • Organizer: 65th Annual Meeting of American Academy of Allergy, Asthma & Immunology
  • Place of Presentation: Washington DC, USA
• [Presentation] アレルギー性炎症における好酸球-集積機序と病的意義の再考 (2008)
  • Author(s): 松本健治
  • Organizer: 第58回日本アレルギー学会総会
  • Place of Presentation: 東京
• [Presentation] ヒト好酸球のamphiregulin産生に関する研究 (2008)
  • Author(s): 松本健治、斎藤博久
  • Organizer: 第20回日本アレルギー学会春期臨床大会
  • Place of Presentation: 東京
• [Presentation] 好酸球のMacrophage-Colony Stimulating Factor (M-CSF) 産生に関する研究 (2008)
  • Author(s): 松本健治、斎藤博久
  • Organizer: 第111回日本小児科学会総会
  • Place of Presentation: 東京
• [Book] Eosinophils in Health and Disease Chapter VIII. Eosinophil Trafficking Section B. Adhesion molecules : interactions between cells and with the extra cellular matrix (2011)
  • Author(s): Kenji Matsumoto
  • Publisher: International Eosinophil Society (IES) In Press
• [Book] Eosinophils in Health and Disease. Chapter VIII. Eosinophil Trafficking Section B. Adhesion molecules : interactions between cells and with the extracellular matrix.
  • Author(s): Matsumoto K, Bochner BS.
  • Publisher: 2011 International Eosinophil Society.(In Press)
• [Remarks] 国立成育医療研究センター研究所免疫アレルギー研究部のホームページ
  • URL: http://111.89.135.117/imal/default.htm