| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
C. difficile is an important pathogen of nosocomial infection, and outbreak of C.difficile associated diseases (CDAD) often occurs in hospitals. However, the pathogenesis of CDAD remains to be determined. We investigated whether C. difficile flagellin were involved in the pathogenesis of CDAD. C. difficile flagellin induced activation of NF-kappaB and p38 mitogen-activated Protein Kinase (MAPK) via TLR5, and also promoted productions of Interleukin-8 (IL-8) and CCL20 in intestinal epithelial cells via TLR5. Pretreatment with toxin B stimulation enhanced production of CCL20 induced by C. difficile flagellin by increasing expressions of TLR5 in intestinal epithelial cells. These results collectively indicated that C. difficile flagellin plays an important role in the pathogenesis of CDAD.
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