Novel oral vaccine using genetically engineering bifidobacterium displaying antigen on its cell-surface

• Project/Area Number: 20591201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kobe University
• Principal Investigator: SHIRAKAWA Toshiro Kobe University, 大学院・保健学研究科, 准教授 (70335446)
• Co-Investigator(Kenkyū-buntansha): SHIMADA Masaru 横浜市立大学, 大学院・医学研究科, 准教授 (40301452) ; KATAYAMA Tkakane 石川県立大学, 生物資源工学研究所, 准教授 (70346104)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: ワクチン / 腸チフス / 粘膜免疫 / ビフィズス菌

Research Abstract

Genetically modified probiotic bacteria show promise as an antigen delivery vehicle for mucosal immunization. We developed a novel vaccine platform utilizing Bifidobacterium as an antigen delivery vehicle for mucosal immunization. Genetically modified Bifidobacterium longum displaying Salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. We confirmed the efficacy of this oral vaccine in a murine typhoid fever model.

Research Products

• [Journal Article] Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model (2010)
  • Author(s): Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, Lee KM, Kawabata M, Shirakawa T.
  • Journal Title: Vaccine Vol.28、No.41
• [Journal Article] Genetically modified Bifidobacterium displaying Salmonella-antigen protects mice from lethal challenge of Salmonella Typhimurium in a murine typhoid fever model (2010)
  • Author(s): Yamamoto S, Wada J, Katayama T, Jikimoto T, Nakamura M, Kinoshita S, Lee KM, Kawabata M, Shirakawa T.
  • Journal Title: Vaccine Volume: 28 Pages: 6684-6691
  • Peer Reviewed
• [Presentation] Genetically Engineered Bifidobacterium Displaying Salmonella-Antigen Protects Mice from Lethal Challenge of Salmonella Typhimurium in a Murine Typhoid Fever Model (2010)
  • Author(s): Toshiro Shirakawa, Sakura Yamamoto, Jun Wada, Takane Katayama, Takumi Jikimoto, Masakuni Nakamura, Shohiro Kinoshita, Kyung-Mi Lee, Masato Kawabata
  • Organizer: American Society of Gene and Cell Therapy 13th annual meeting
  • Place of Presentation: ワシントンDC(米国)
  • Year and Date: 2010-05-20
• [Presentation] Genetically Engineered Bifidobacterium Displaying Salmonella-Antigen Protects Mice from Lethal Challenge of Salmonella Typhimurium in a Murine Typhoid Fever Model (2010)
  • Author(s): Toshiro Shirakawa, Sakura Yamamoto, Jun Wada, Takane Katayama, Takumi Jikimoto, Masakuni Nakamura, Shohiro Kinoshita, Kyung-Mi Lee, Masato Kawabata
  • Organizer: American Society of Gene and Cell Therapy 13th annual meeting
  • Place of Presentation: ワシントンDC、米国
  • Year and Date: 2010-05-20
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] ビフィズス菌表層提示融合タンパク質発現遺伝子 (2009)
  • Inventor(s): 白川利朗、山本さくら、片山高嶺、和田潤、加納康正、島本康介、浅田雅宣
  • Industrial Property Rights Holder: 神戸大学、森下仁丹(株)
  • Industrial Property Number: 2009-216256
  • Filing Date: 2009-09-17
• [Patent(Industrial Property Rights)] ビフィズス菌表層提示融合タンパク質発現遺伝子 (2009)
  • Inventor(s): 白川利朗, 山本さくら, 片山高嶺, 和田潤, 加納康正, 島本康介, 浅田雅宜
  • Industrial Property Rights Holder: 神戸大学, 森下仁丹(株)
  • Industrial Property Number: 2009-216256
  • Filing Date: 2009-09-17