Molecular biological study on macrophage antimicrobial mechanism based on phospholipase A_2
| Project/Area Number |
20591202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Shimane University |
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Principal Investigator |
TOMIOKA Haruaki Shimane University, 医学部, 教授 (40034045)
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| Co-Investigator(Kenkyū-buntansha) |
SANO Chiaki 島根大学, 医学部, 准教授 (70325059)
TATANO Yutaka 島根大学, 医学部, 助教 (70432614)
SHIMIZU Toshiaki 安田女子大学, 家政学部, 准教授 (60284030)
YASUMOTO Ko 北里大学, 海洋生命科学部, 講師 (00448200)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マクロファージ / 抗酸菌 / 殺菌メカニズム / phospholipase A_2 / cPLA_2 / 遊離脂肪酸 |
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| Research Abstract |
We investigated macrophage (MΦ) antimicrobial activity against mycobacteria based on phospholipase A_2 (PLA_2) using molecular biological techniques. (1) Among PLA_2 isotype inhibitors, cytosolic PLA_2 (cPLA_2) inhibitor mildly reduced MΦ anti-mycobacteria activity. (2) MΦ expression of cPLA_2 and secretoryPLA_2-V mRNAs were up-regulated after infection with mycobacteria. (3) cPLA_2 potentiated M Φ anti-mycobacteria activity coupled with apoptosis in the early stage. (4) GFP-tagged cPLA_2 translocated to the membrane of MΦ or CHO cells. Overall, it can be concluded that the cPLA_2-mediated antimicrobial mechanism contributes to MΦ's bactericidal and bacteriostatic function against mycobacterial pathogens but such cPLA_2-dependent mechanism does not play a central role in the MΦ antimycobacterial function.
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Report
(4 results)
Research Products
(109 results)
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[Journal Article] Experimental and Clinical Studies on Rifacunna -The New Effective Antituberculous Drug-(Review)2010
Author(s)
Dimova V, Atanasova I, Tomioka H, Sato K, Reddy V, Geeta N, Daneluzzi D, Gangadharam P, Kantardjiev T, Dhople A, Feshchenko Y, Yashina L, Toumanov A, Zhivkova Z, Sano C
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Journal Title
Recent Patents on Anti-Infective Drug Discovery (In press)
Related Report
Peer Reviewed
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