| Project/Area Number |
20591202
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Infectious disease medicine
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| Research Institution | Shimane University |
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Principal Investigator |
TOMIOKA Haruaki Shimane University, 医学部, 教授 (40034045)
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| Co-Investigator(Kenkyū-buntansha) |
SANO Chiaki 島根大学, 医学部, 准教授 (70325059)
TATANO Yutaka 島根大学, 医学部, 助教 (70432614)
SHIMIZU Toshiaki 安田女子大学, 家政学部, 准教授 (60284030)
YASUMOTO Ko 北里大学, 海洋生命科学部, 講師 (00448200)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | マクロファージ / 抗酸菌 / 殺菌メカニズム / phospholipase A_2 / cPLA_2 / 遊離脂肪酸 |
|---|
| Research Abstract |
We investigated macrophage (MΦ) antimicrobial activity against mycobacteria based on phospholipase A_2 (PLA_2) using molecular biological techniques. (1) Among PLA_2 isotype inhibitors, cytosolic PLA_2 (cPLA_2) inhibitor mildly reduced MΦ anti-mycobacteria activity. (2) MΦ expression of cPLA_2 and secretoryPLA_2-V mRNAs were up-regulated after infection with mycobacteria. (3) cPLA_2 potentiated M Φ anti-mycobacteria activity coupled with apoptosis in the early stage. (4) GFP-tagged cPLA_2 translocated to the membrane of MΦ or CHO cells. Overall, it can be concluded that the cPLA_2-mediated antimicrobial mechanism contributes to MΦ's bactericidal and bacteriostatic function against mycobacterial pathogens but such cPLA_2-dependent mechanism does not play a central role in the MΦ antimycobacterial function.
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