Suppression of viral replication and the innate immune response of interleukin27

• Project/Area Number: 20591208
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Infectious disease medicine
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TSUNAO Kishida Kyoto Prefectural University of Medicine, 医学研究科, 助教 (00370205)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 感染症治療学 / ウイルス / 感染症 / 微生物 / サイトカイン

Research Abstract

Interleukin-17 (IL-27) plays a central role in induction of Th1 immune response, while its contribution to anti-virus innate immunity remains unknown. We found that IL-27 directly acts on cardiomyocytes and strongly inhibits infection andamplification of the encephalomyocarditis virus (EMCV). The antiviral activity of IL-27 is mediated through regulation of virus sensor molecules and accelerationof type I interferon induction. The present results may lead to development of a novel antiviral vaccine.

Research Products

• [Journal Article] Interleukin-28B acts synergistically with cisplatin to suppress the growth of head and neck squamous cell carcinoma. (2011)
  • Author(s): Yoshimoto K, Kishida T, Nakano Hi, Matsui M, Shin-Ya M, Shimada T, Nakai S, Imanishi J, Takeuchi M, Hisa Y, Mazda O.
  • Journal Title: J Immunother 34(2) Pages: 139-148
  • Peer Reviewed
• [Journal Article] Intra-articular injection of hyaluronan restores the aberrant expression of matrix metalloproteinase-13 in osteoarthritic subchondral bone. (2011)
  • Author(s): Hiraoka N, Takahashi KA, Arai Y, Sakao K, Mazda O, Kishida T, Honjo K, Tanaka S, Kubo T.
  • Journal Title: J Orthop Res 29(3) Pages: 354-360
  • Peer Reviewed
• [Journal Article] IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or lgE Production. (2011)
  • Author(s): Tamagawa-Mineoka R, Kishida T, Mazda O, Katoh N.
  • Journal Title: J Invest Dermatol. Volume: 24 Pages: 1513-1520
  • Peer Reviewed
• [Journal Article] Interleukin-28B acts synergistically with cisplatin to suppress the growth of head and neck squamous cell carcinoma. (2011)
  • Author(s): Yoshimoto K, Kishida T, Nakano H, Matsui M, Shin-Ya M, Shimada T, Nakai S, Imanishi J, Takeuchi M, Hisa Y, Mazda O.
  • Journal Title: J Immunother Volume: 34 Pages: 139-148
  • Peer Reviewed
• [Journal Article] Intra-articular injection of hyaluronan restores the aberrant expression of matrix metalloproteinase-13 in osteoarthritic subchondral bone. (2011)
  • Author(s): Hiraoka N, Takahashi KA, Arai Y, Sakao K, Mazda O, Kishida T, Honjo K, Tanaka S, Kubo T.
  • Journal Title: J Orthop Res Volume: 29 Pages: 354-360
  • Peer Reviewed
• [Journal Article] Mitf silencing cooperates with IL-12 gene transfer to inhibit melanoma in mice. (2010)
  • Author(s): Nakai N, Kishida T, Hartmann G, Katoh N, Imanishi J, Kishimoto S, Mazda, O.
  • Journal Title: Int Immunopharmacol 10(4) Pages: 540-545
  • Peer Reviewed
• [Journal Article] Early-stage blocking of Notch signaling inhibits the depletion of goblet cells in dextran sodium sulfate-induced colitis in mice. (2010)
  • Author(s): Shinoda M, Shin-Ya M, Naito Y, Kishida T, Ito R, Suzuki N, Yasuda H, Sakagami J, Imanishi J, Kataoka K, Mazda O, Yoshikawa T.
  • Journal Title: J.Gastroenterol 45(6) Pages: 608-617
  • NAID: 10027216548
  • Peer Reviewed
• [Journal Article] lEarly-stage blocking of Notch signaling inhibits the depletion of goblet cells in dextran sodium sulfate-induced colitis in mice. (2010)
  • Author(s): Shinoda M, Shin-Ya M, Naito Y, Kishida T, Ito R, Suzuki N, Yasuda H, Sakagami J, Imanishi J, Kataoka K, Mazda O, Yoshikawa T
  • Journal Title: J Gastroenterol Volume: 45 Pages: 608-617
  • Peer Reviewed
• [Journal Article] Mitf silencing cooperates with IL-12 gene transfer to inhibit melanoma in mice. (2010)
  • Author(s): Nakai N, Kishida T, Hartmann G, Katoh N, Imanishi J, Kishimoto S, Mazda O
  • Journal Title: Int Immunopharmacol Volume: 10 Pages: 540-545
  • Peer Reviewed
• [Journal Article] N-acetylcysteine prevents nitric oxide-induced chondrocyte apoptosis and cartilage degeneration in an experimental model of osteoarthritis. (2010)
  • Author(s): Nakagawa S, Arai Y, Mazda O, Kishida T, Takahashi KA, Sakao K, Saito M, Honjo K, Imanishi J, Kubo T.
  • Journal Title: J Orthop Res. 28(2) Pages: 156-163
  • Peer Reviewed
• [Journal Article] MDR1a/1b gene silencing enhances drug sensitivity in rat fibroblast-like synoviocytes. (2010)
  • Author(s): Honjo K, Takahashi KA, Mazda O, Kishida T, Shinya M, Tokunaga D, Arai Y, Inoue A, Hiraoka N, Imanishi J, Kubo T.
  • Journal Title: J Gene Med. 12(2) Pages: 219-227
  • Peer Reviewed
• [Journal Article] Mitf silencing cooperates with IL-12 gene transfer to inhibit melanoma in mice. (2010)
  • Author(s): Nakai N, Kishida T, Hartmann G, Katoh N, Imanishi J, Kishimoto S, Mazda O.
  • Journal Title: Int Immunopharmacol. 10(4) Pages: 540-545
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of peptide-induced delayed-type hypersensitivity in advanced melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination. (2009)
  • Author(s): Nakai N, Katoh N, Germeraad WT, Kishida T, Ueda E, Takenaka H, Mazda O, Kishimoto S.
  • Journal Title: J.Dermatol.Sci 53(1) Pages: 40-47
  • NAID: 10025331985
  • Peer Reviewed
• [Journal Article] Comparison of anti-rheumatic effects of local RNAi-based therapy in collagen induced arthritis rats using various cytokine genes as molecular targets. (2009)
  • Author(s): Inoue A, Takahashi KA, Mazda O, Arai Y, Saito M, Kishida T, Shin-Ya M, Morihara T, Tonomura H, Sakao K, Imanishi J, Kubo T.
  • Journal Title: Mod Rheumatol 19(2) Pages: 125-133
  • NAID: 10025112341
  • Peer Reviewed
• [Journal Article] Osteoblasts derived from osteophytes produce inteleukin-6, interleukin-8 and matrix metalloproteinase-13 in osteoarthritis. (2009)
  • Author(s): Sakao, K., K.A. Takahashi, Y. Arai, M. Saito, K. Honjo, N. Hiraoka, H. Asada, M. Shin-Ya, J. Imanishi, O. Mazda, T. Kubo.
  • Journal Title: J Bone Miner Metab 27(4) Pages: 412-423
  • Peer Reviewed
• [Journal Article] Interleukin-27 activates natural killer cells and suppresses NK-resistant head and neck squamous cell carcinoma through inducing antibody-dependent cellular cytotoxicity. (2009)
  • Author(s): Matsui M, Kishida T, Nakano H, Yoshimoto K, Shin-Ya M, Shimada T, Nakai S, Imanishi J, Yoshimoto T, Hisa Y, Mazda O.
  • Journal Title: Cancer Res 69(6) Pages: 2523-2530
  • Peer Reviewed
• [Journal Article] Raspberry-like assembly of cross-linked nanogels for protein delivery. (2009)
  • Author(s): Hasegawa U, Sawada SI, Shimizu T, Kishida T, Otsuji E, Mazda O, Akiyoshi K.
  • Journal Title: J Control Release 140(3) Pages: 312-317
  • Peer Reviewed
• [Journal Article] Raspberry-like assembly of cross-linked nanogels for protein delivery. (2009)
  • Author(s): Hasegawa U, Sawada S, Shimizu T, Kishida T, Otsuji E, Mazda O, Akiyoshi K.
  • Journal Title: J Control Release 140(3) Pages: 312-317
  • Peer Reviewed
• [Journal Article] Asporin and transforming growth factor-beta gene expression in osteoblasts from subchondral bone and osteophytes in osteoarthritis. (2009)
  • Author(s): Sakao K, Takahashi KA, Arai Y, Saito M, Honjyo K, Hiraoka N, Kishida T, Mazda O, Imanishi J, Kubo T.
  • Journal Title: J Orthop Sci. 14(6) Pages: 738-747
  • Peer Reviewed
• [Journal Article] Immunohistological analysis of peptide-induced delayed-type hyper sensitivity in advanced melanoma patients treated with melanoma antigen-pulsed mature monocyte-derived dendritic cell vaccination. (2009)
  • Author(s): Nakai N, Katoh N, Germeraad WT, Kishida T, Mazda 0, Kishimoto S., et al
  • Journal Title: J Dermatol Sci. 53
  • NAID: 10025331985
  • Peer Reviewed
• [Journal Article] Involvement of IL-17A in the pathogenesis of DSS-induced colitis in mice. (2008)
  • Author(s): Ito R, Kita M, Shin-Ya M, Kishida T, Urano A, Takada R, Sakagami J, Imanishi J, Iwakura Y, Okanoue T, Yoshikawa T, Kataoka K, Mazda O.
  • Journal Title: Biochem Biophys Res Commun 377(1) Pages: 12-16
  • Peer Reviewed
• [Journal Article] Nanogel DDS enables sustained release of IL-12 for tumor immun otherapy. (2008)
  • Author(s): Shimizu T, Kishida T, Mazda 0., et al
  • Journal Title: Biochem Biophys Res Commun. 377 Pages: 330-335
  • Peer Reviewed
• [Journal Article] IL-21 Reduces Immediate Hypersensitivity Reactions in Mouse Skin by Suppressing Mast Cell Activation or IgE Production.
  • Author(s): Tamagawa-Mineoka R, Kishida T, Mazda O, Katoh N.
  • Journal Title: J Invest Dermatol in press
  • Peer Reviewed
• [Journal Article] Early-stage blocking of Notch signaling inhibits the depletion of goblet cells in dextran sodium sulfate-induced colitis in mice.
  • Author(s): Shinoda M, Shin-Ya M, Naito Y, Kishida T, Ito R, Suzuki N, Yasuda H, Sakagami J, Imanishi J, Kataoka K, Mazda O, Yoshikawa T.
  • Journal Title: J Gastroenterol. (印刷中)
  • NAID: 10027216548
  • Peer Reviewed
• [Journal Article] lnterleukin-27 activates natural killer cells and suppresses NK-resistant head and neck squamous cell carcinoma through inducing antibody-dependent cellular cytotoxicity.
  • Author(s): Matsui M, Kishida T, Mazda O, et al
  • Journal Title: Cancer Research (In press)
  • Peer Reviewed
• [Presentation] Gain-of-function and loss-of-function analyses in vivo of transcriptional factor and cytokine genes using Epstein-Barr virus-based episomal vectors, and their implication to novel strategies of gene therapy and regenerative medicine. (2010)
  • Author(s): Kishida T, Nakai N, Matsui M, Yoshimoto1 K, Nakano H, Shin-Ya M, Shimada T, Nakai S, Hisa Y, Katoh N, Mazda O
  • Organizer: 2010 International Symposium on Micro-Nano Mechatronics and Human Science (From Micro & Nano Scale Systems to Robotics & Mechatronics Systems)
  • Place of Presentation: Nagoya
• [Presentation] IL-21の多彩なin vivo 生理活性と分子病態制御への応用 (2009)
  • Author(s): 岸田綱郎、菅井学、清水章、今西二郎、横田義史、松田修
  • Organizer: 免疫カンファレンス
  • Place of Presentation: 京都
  • Year and Date: 2009-11-21
• [Presentation] IL-21の多彩なin vivo生理活性と分子病態制御への応用 (2009)
  • Author(s): 岸田綱郎、菅井学、清水章、今西二郎、横田義史、松田修
  • Organizer: 免疫ワークショップ
  • Place of Presentation: 京都
  • Year and Date: 2009-11-21
• [Presentation] Nonviral gene administration by means of the Epstein-Barr virus (EBV)-based episomal vectors and its application to gene therapy and regener ative medicine (2009)
  • Author(s): Tsunao Kishida, Osam Mazda, Masahiro Matsui, Hiroshi Nakano, Koichiro Yoshimoto, Taketoshi Shimada, Shigeru Nakai, Jiro Imanishi, Yasuo Hisa
  • Organizer: MHS2009
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-10
• [Presentation] IL-21 によるマウスにおけるアナフィラキシー抑制効果 (2008)
  • Author(s): 広村弥生、岸田綱郎、安田誠、濱雄光、松田修、久育男
  • Organizer: 第57回日本アレルギー学会
  • Place of Presentation: 東京
  • Year and Date: 2008-11-28
• [Presentation] IL-21 の経鼻的投与によるアレルギー性鼻炎の抑制 (2008)
  • Author(s): 広村弥生、岸田綱郎、中野宏、濱雄光、松田修、久育男
  • Organizer: 第109回日本耳鼻咽喉科学会
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-16
• [Presentation] IL-21 administration into the nostril alleviates murine allergic rhinitis. (2008)
  • Author(s): 広村弥生, 岸田綱郎, 中野宏, 濱雄光, 松田修, 久育男
  • Organizer: The 12th Japan-Korean joint meeting of Otorhinolaryngology-Head and Neck Surgery
  • Place of Presentation: Nara
  • Year and Date: 2008-04-05
• [Presentation] IL-21 はB細胞にinhibitor of differentiation 2発現を誘導してアレルギーを抑制する (2008)
  • Author(s): 岸田綱郎、菅井学、清水章、今西二郎、横田義史、松田修
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都