| Project/Area Number |
20591227
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
YAMASHITA Michitoshi Fukushima Medical University, 医学部, 助教 (20381387)
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| Co-Investigator(Kenkyū-buntansha) |
ISE Kazuya 福島県立医科大学, 医学部, 講師 (90363746)
SATOH Yoshihiro 福島県立医科大学, 医学部, 博士研究員 (60347218)
ISHII Shou 福島県立医科大学, 医学部, 助教 (40468129)
MATSUYAMA Shinichi 福島県立医科大学, 医学部, 博士研究員 (90381384)
GOTOH Mitsukazu 福島県立医科大学, 医学部, 教授 (50162160)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | I型糖尿病 / 膵島移植 / 膵島再生 / 細胞シート / ティッシュエンジニアリング / 型糖尿病 / 再生膵島 |
|---|
| Research Abstract |
After reaching confluency on temperature-responsive culture dishes, cultured rat islet cells were harvested as a uniformly connected tissue sheet by lowering culture temperature. Histological examination showed that harvested cell sheet had a monolayered 2-D structure, which established and retained its structural cell-to-cell connections. Immunohistological staining revealed that islet cell sheet comprised of insulin- and glucagon- staining positive cells. By transplanting islet cell sheets into the subcutaneous space of mice, islet tissues were successfully engineered and sustained. Therapeutic effectiveness of this islet bioengineering procedure was also confirmed in the transplantation study to induced diabetic mice.
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