The role of the mitochondrial targeting signal in the function of steroidogenic acute regulatory protein revealed by BAC transgenesis in vivo

Research Project

Project/Area Number	20591231
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Pediatrics
Research Institution	Keio University
Principal Investigator	ISHII Tomohiro Keio University, 医学部, 助教 (70265867)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords	steroidogenic acute regulatory protein / ステロイドホルモン / 細菌人工染色体(BAC) / トランスジェニックマウス / ミトコンドリア標的シグナル / 細菌人工染色体
Research Abstract	The present study clarified the mechanism of steroidogenic acute regulatory protein (StAR) that transfers cholesterol from the outer to the inner mitochondrial membrane in the first step of acute steroidogenesis. It demonstrated the important role of mitochondrial entry of StAR by its mitochondrial targeting signal on the function of the StAR in vivo.