Project/Area Number |
20591236
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | University of Occupational and Environmental Health, Japan |
Principal Investigator |
YAMAMOTO Yukiyo University of Occupational and Environmental Health, Japan, 医学部, 講師 (20279334)
|
Co-Investigator(Kenkyū-buntansha) |
DOBASHI Kazushige 昭和大学, 医学部, 講師 (60260569)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥130,000 (Direct Cost: ¥100,000、Indirect Cost: ¥30,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
Keywords | Nesfatin-1 / 室傍核 / 弓状核 / 摂食調節 / 生後発達 / 脳脂肪細胞系 / 視床下部 / 視索上核 / Galanin-like peptide / Neuromedin U / 下垂体後葉 / 下垂体前葉 / 浸透圧調節 |
Research Abstract |
To begin to understand the physiological role of Nesfatin-1 during postnatal development, we examined the developmental changes of the Nesfatin-1 gene expression in the PVN, SON and ARC during postnatal day 1 to day 60, using in situ hybridization histochemistry. Development of Nesfatin-1, which showed different patterns each hypothalamic nucleus during postnatal development, may be associated with its region-specific roles during postnatal development.Next, we examined the effect of the maternal deprivation for twenty-four hours on the gene expression of the Nesfatin-1 during pre-weaning period. After 24 h milk deprivation, the Nesfatin-1 gene expression was significantly increased at postnatal day 15. Significant increase in the expression of the Nesfatin-1 gene after milk deprivation suggested that osmolality change induced by milk deprivation might be a stimulant to induce the expression of the Nesfatin-1 gene in the preweaning period. Development of Nesfatin-1 may be associated with its critical role in the maturation of neurosecretory function in the hypothalamus-pituitary axis.
|