Roles of neuroglycan C on dendritic spine formation

• Project/Area Number: 20591240
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Institute for Developmental Research, Aichi Human Service Center
• Principal Investigator: TOKITA Yoshihito 愛知県心身障害者コロニー発達障害研究所, 周生期学部, 主任研究員 (50291175)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: コンドロイチン硫酸 / プロテオグリカン / シナプス / スパイン / シナプス形成 / 増殖因子 / 神経細胞 / ニューログリカンC

Research Abstract

Neuroglycan C(NGC) is a transmembrane chondroitin sulfate proteoglycan that is expressed in the brain and predominantly localizes dendritic filopodia on neuron. Although it is revealed that presynaptic function of NGC-null mice was partially impaired in early stages of development, the molecular mechanisms of synapse formation modulated by NGC proteoglycan remains undefined. To address this question, we sought to determine the identity of proteins that interact with NGC. As the result of the analysis, we identified several members in the FGF family. In addition to a broad mitogenic and other biological processes including neural morphogenesis, some of members in FGF family were reported as a presynaptic organizer protein, which can induce a formation of functional synaptic vesicle aggregates on axon fiber. The result suggests a possibility that NGC on the dendrites functions to display FGF as a co-receptor for FGFR on the axons to form functional synapse at appropriate sites.

Research Products

• [Journal Article] Clinical and functional data implicate the Arg(151) Ser variant of MSX1 in Familial Hypodontia (2011)
  • Author(s): Kamamoto M, Machida J, Yamaguchi S, Kimura M, Ono T, Jezewski PA, Higashi Y, Nakayama A, Shimozato K, Tokita Y.
  • Journal Title: Euro J Hum Genet Volume: 19 Issue: 8 Pages: 844-50
  • DOI: 10.1038/ejhg.2011.47
  • Peer Reviewed
• [Journal Article] Clinical and functional data implicate the Arg (151) Ser variant of MSX1 in Familial Hypodontia (2011)
  • Author(s): Kamamoto M, Machida J, et al
  • Journal Title: Euro J Hum Genet Volume: 19 Pages: 844-850
  • Peer Reviewed
• [Journal Article] A novel mutation in the C-terminal region of RUNX2/CBFA1 distal to the DNA-binding runt domain in a Japanese patient with cleidocranial dysplasia (2010)
  • Author(s): Kamamoto M, Machida J, Miyachi H, Ono T, Nakayama A, Shimozato K, Tokita Y.
  • Journal Title: Int J Oral Maxillofac Surg Volume: 40 Issue: 4 Pages: 434-437
  • DOI: 10.1016/j.ijom.2010.09.025
• [Journal Article] Neuroglycan C, a brain-specific chondroitin sulfate proteoglycan, interacts with pleiotrophin, a heparin-binding growth factor (2010)
  • Author(s): Nakanishi K, Tokita Y, Aono S, Ida M, Matsui F, Higashi Y, Oohira A.
  • Journal Title: Neurochem Res Volume: 35 Pages: 1131-7
• [Journal Article] Reduction of brain injury in neonatal hypoxic-ischemic rats by intracerebroventricular injection of neural stem/progenitor cells together with chondroitinase ABC (2008)
  • Author(s): Sato Y, Nakanishi K, Hayakawa M, Kakizawa H, Saito A, Kuroda Y, Ida M, Tokita Y, Aono S, Matsui F, Kojima S, Oohira A.
  • Journal Title: Reprod Sci Volume: 15 Issue: 6 Pages: 613-20
  • DOI: 10.1177/1933719108317299
• [Journal Article] Differential effects of two ROCK inhibitors, Fasudil and Y-27632, on optic nerve regeneration in adult cats (2008)
  • Author(s): Ichikawa M, Yoshida J, Saito K, Sagawa H, Tokita Y, Watanabe M.
  • Journal Title: Brain Res Volume: 1201 Pages: 23-33
  • DOI: 10.1016/j.brainres.2008.01.063
• [Journal Article] A highly sulfated chondroitin sulfate preparation, CS-E, prevents excitatory amino acid-induced neuronal cell death (2008)
  • Author(s): Sato Y, Nakanishi K, Hayakawa M, Kakizawa H, Saito A, Kuroda Y, Ida M, Tokita Y, Aono S, Matui F, Kojima S, Oohira A.
  • Journal Title: J Neurochem Volume: 104 Issue: 6 Pages: 1565-76
  • DOI: 10.1111/j.1471-4159.2007.05107.x
• [Journal Article] Differential effects of two ROCK inhibitors, Fasudil and Y-27632, on optic nerve regeneration in adult cats (2008)
  • Author(s): Ichikawa M, Yoshida J, Saito K, Sagawa H, Tokita Y, Watanabe M.
  • Journal Title: Brain Res. 1201 Pages: 23-33
  • Peer Reviewed
• [Presentation] Variable Penetrance, Expressivity and Pleiotropy of MSX1 Alleles (2010)
  • Author(s): Jezewski PA, Houseman EA, Kamamoto M, Machida J, Yamaguchi S, Ono T, Higashi Y, Nakayama A, Shimozato K, Tokita Y
  • Organizer: 31st Annual David W. Smith Workshop on malformations and morphogenesis
  • Place of Presentation: Union, Washington State
  • Year and Date: 2010-08-28
• [Presentation] Neuroglycan Cによる神経突起の形態制御 (2008)
  • Author(s): 時田義人
  • Organizer: 日本生化学会・日本分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] 先天性多数歯欠損症におけるMSX1、PAX9遺伝子の変異解析 (2008)
  • Author(s): 釜本宗史
  • Organizer: 日本生化学会・日本分子生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Role of Neuroglycan C, a Brain-specific Proteoglycan, in Neuronal Development (2008)
  • Author(s): Tokita Y, Aono S, Matsui F, Nakanishi K, Oohira A.
  • Organizer: The 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: Invited, Jeju, Korea
  • Year and Date: 2008-11-04
• [Presentation] Role of Neuroglycan C, a Brain-specific Proteoglycan, in Neuronal Development (2008)
  • Author(s): TOKITA Yoshihito
  • Organizer: The 9th Asia-Pacific Microscopy Conference
  • Place of Presentation: Jeju (Korea)
  • Year and Date: 2008-11-04