The cytotoxic effect by natural killer cells against pediatric malignant tumors.

• Project/Area Number: 20591249
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: University of Yamanashi
• Principal Investigator: GOI Kumiko University of Yamanashi, 大学院・医学工学総合研究部, 助教 (70324192)
• Co-Investigator(Kenkyū-buntansha): OSHIRO Hiroko 山梨大学, 医学部附属病院, 医員 (50377537)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: NK細胞 / 難治性腫瘍 / 白血病 / KIR / 同種移植 / GVL効果 / KIRリガンド / 急性リンパ性白血病 / 臍帯血

Research Abstract

We analyzed the effect and the mechanisms of the cytotoxicity of the peripheral blood (PB) and the cord blood (CB) NK cells against pediatric leukemia and solid tumors. If donor KIR (killer cell Ig-like receptor) ligand is not present in the tumor cells, alloreactivity against the recipients' tumor cells is increased in MLL rearranged acute lymphoblastic leukemia and T-cell acute lymphoblastic leukemia. Therefore the graft versus tumor effect against these malignancies could be considerably expected if the KIR ligand incompatible donor is selected for CBT.

Research Products

• [Journal Article] Successful tandem (autologous-cord blood) SCT in advanced neuroblastomas with highly amplified MYCN. (2010)
  • Author(s): Goi K, Inukai T, Honna H, et al
  • Journal Title: Bone Marrow Transplant.[Epub ahead of print]
  • Peer Reviewed
• [Presentation] The cytotoxicity of cord blood NK cells against MLL-rearramged ALL is associated with SNPs of NKG2D (2010)
  • Author(s): 本名浩子、合井久美子
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2010-09-24
• [Presentation] 同種臍帯血NK細胞のT 細胞性急性リンパ性白血病に対する細胞傷害活性に関する検討 (2009)
  • Author(s): 本名浩子、合井久美子
  • Organizer: 日本小児血液学会
  • Place of Presentation: 千葉県浦安市
  • Year and Date: 2009-11-27
• [Presentation] 同種臍帯血NK細胞のT細胞性急性リンパ性白血病に対する細胞傷害活性に関する検討 (2009)
  • Author(s): 本名浩子
  • Organizer: 日本児血液学会
  • Place of Presentation: 千葉県浦安市
  • Year and Date: 2009-11-27
• [Presentation] 同種臍帯血NK細胞のMLL遺伝子再構成陽性ALLに対する細胞傷害活性に関する検討 (2008)
  • Author(s): 本名浩子、合井久美子
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-10-11