Analysis of the variety of chimeric genes in leukemic cel ls

• Project/Area Number: 20591257
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kyoto Prefectural University of Medicine
• Principal Investigator: TAKI Tomohiko Kyoto Prefectural University of Medicine, 医学研究科, 講師 (50322053)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 白血病 / 悪性リンパ腫 / 多発性骨髄腫 / 染色体転座 / キメラ遺伝子 / スプライシング異常 / 遺伝子 / 癌

Research Abstract

We have identified three novel chimeric transcripts, MOZ-LEUTX, PAX5-FOXP2, IGH-BACH2, in leukemia or lymphoma samples. We also identified novel types of abnormal transcripts of DCC gene in multiple myeloma. Further analysis of these transcripts will reveal the unknown mechanism in tumorigenesis of various types of malignancies.
白血病

Research Products

• [Journal Article] Identification of IGHCδ-BACH2 fusion transcripts resulting from cryptic chromosomal rearrangements of 14q32 with 6q15 in aggressive B-cell lymphoma/leukemia. (2011)
  • Author(s): Kobayashi S, Taki T, Chinen Y, Tsutsumi Y, Ohshiro M, Kobayashi T, Matsumoto Y, Kuroda J, Horiike S, Nishida K, Taniwaki M.
  • Journal Title: Genes Chromosomes Cancer 50 Pages: 207-216
• [Journal Article] Spinal cord compression by epidural involvement over 21 vertebral levels in acute lymphoblastic leukemia. (2011)
  • Author(s): some K, Matsubara K, Taki T, Nigami H, Yura K, Iwata A, Wada T, Taniwaki M, Fukaya T.
  • Journal Title: J Pediatr Hemtol Oncol 33 Pages: 153-157
• [Journal Article] Identification of IGHC δ -BACH2 fusion transcripts resulting from cryptic chromosomal rearrangements of 14q32 with 6q15 in aggressive B-cell lymphoma/leukemia. (2011)
  • Author(s): Kobayashi S, et al.
  • Journal Title: Genes Chromosomes Cancer Volume: 50 Pages: 207-216
  • Peer Reviewed
• [Journal Article] Spinal cord compression by epidural involvement over 21 vertebral levels in acute lymphoblastic leukemia. (2011)
  • Author(s): Isome K, et al.
  • Journal Title: J Pediatr Hemtol Oncol Volume: 33 Pages: 153-157
  • Peer Reviewed
• [Journal Article] FLT3-internal tandem duplication in a pediatric patient with t(8;21) acute myeloid leukemia. (2010)
  • Author(s): Kawamura M, Kaku H, Ito C, Funada N, Taki T, Shimada A, Hayashi Y.
  • Journal Title: Cancer Genet Cytogenet 203 Pages: 292-296
• [Journal Article] High frequencies of simultaneous FLT3-ITD and WT1 mutations in myeloid leukemia with NUP98-HOX fusion genes. (2010)
  • Author(s): Taketani T, Taki T, Nakamura T, Ohyashiki K, Kobayashi Y, Fukuda S, Yamaguchi S, Hayashi Y.
  • Journal Title: Leukemia 24 Pages: 1975-1977
• [Journal Article] Prognostic significance of the BAALC isoform pattern and CEBPA mutations in pediatric acute myeloid leukemia with normal karyotype: a study by the Japanese Childhood AML Cooperative Study Group. (2010)
  • Author(s): Mizushima Y, Taki T, Shimada A, Yui M, Hiraumi Y, Matsubara H, Watanabe M, Watanabe K, Kamitsuji Y, Hayashi Y, Tsukimoto I, Kobayashi R, Horibe K, Tawa A, Nakahata T, Adachi S.
  • Journal Title: Int J Hematol 91 Pages: 831-837
  • NAID: 10026511260
• [Journal Article] Down-regulation of CDKN1A in adult T cell leukemia/lymphoma despite overexpression of CDKN1A in HTLV-1-infected cell lines. (2010)
  • Author(s): Watanabe M, Nakahata S, Hamasaki M, Saito Y, Kawano Y, Hidaka T, Yamashita K, Umeki K, Taki T, Taniwaki M, Okayama A, Morishita K.
  • Journal Title: J Virol 84 Pages: 6966-6977
• [Journal Article] FLT3-internal tandem duplication in a pediatric patient with t(8;21) acuto myeloid leukemia. (2010)
  • Author(s): Kawamura M, et al.
  • Journal Title: Cancer Genet Cytogenet Volume: 292 Pages: 292-296
  • Peer Reviewed
• [Journal Article] High frequencies of simultaneous FLT3-ITD and WT1 mutations in myeloid leukemia with NUP98-HOX fusion genes. (2010)
  • Author(s): Taketani T, et al.
  • Journal Title: Leukemia Volume: 24 Pages: 1975-1977
  • Peer Reviewed
• [Journal Article] Prognostic significance of the BAALC isoform pattern and CEBPA mutations in pediatric acute myeloid leukemia with normal karyotype : a study by the Japanese Childhood AML Cooperative Study Group. (2010)
  • Author(s): Mizushima Y, et al.
  • Journal Title: Int J Hematol Volume: 91 Pages: 831-837
  • Peer Reviewed
• [Journal Article] Down-regulation of CDKN1A in adult T cell leukemia/lymphoma despite overexpression of CDKN1A in HTLV 1-infected cell lines. (2010)
  • Author(s): Watanabe M, et al.
  • Journal Title: J Virol Volume: 84 Pages: 6966-6977
  • Peer Reviewed
• [Journal Article] MLL fusion protein collaborates with Raf to induce acute leukemia through aberrant Hox expression and Raf activation. (2009)
  • Author(s): Ono R, Kumagai H, Nakajima H, Hishiya A, Taki T, Horikawa K, Takatsu K, Satoh T, Hayashi Y, Kitamura T, Nosaka T.
  • Journal Title: Leukemia 23 Pages: 2197-2209
• [Journal Article] Immunologically silent cancer clone transmission from mother to offspring. (2009)
  • Author(s): Isoda T, Ford AM, Tomizawa D, van Delft FW, Gonzalez De Castro D, Mitsuiki N, Score J, Taki T, Morio T, Takagi M, Saji H, Greaves M, Mizutani S.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 17882-17885
• [Journal Article] Alteration of enhancer of polycomb 1 at 10p11.2 is one of the genetic events for developing adult T-cell leukemia/lymphoma. (2009)
  • Author(s): Nakahata S, Saito Y, Hamasaki M, Hidaka T, Arai Y, Taki T, Taniwaki M, Morishita K.
  • Journal Title: Genes Chromosomes Cancer 48 Pages: 768-776
• [Journal Article] Juvenile myelomonocytic leukaemia with t(7;11)(p15;p15) and NUP98-HOXA11 fusion. (2009)
  • Author(s): Mizoguchi Y, Fujita N, Taki T, Hayashi Y, Hamamoto K.
  • Journal Title: Am J Hematol 84 Pages: 295-297
• [Journal Article] FBXW7 and NOTCH1 mutations in childhood T-cell acute lymphoblastic leukemia and T cell non-Hodgkin's lymphoma. (2009)
  • Author(s): Park M, Taki T, Oda M, Watanabe T, Yumura-Yagi K, Kobayashi R, Suzuki N, Hara J, Horibe K, Hayashi Y.
  • Journal Title: Br J Haematol 145 Pages: 198-206
• [Journal Article] Monosomies 7p and 12p and FLT3 internal tandem duplication : possible markers for diagnosis of T/myeloid biphenotypic acute leukemia and its clonal evolution. (2009)
  • Author(s): Matsumoto Y, Taki T, Fujimoto Y, Taniguchi K, Shimizu D, Shimura K, Uchiyama H, Kuroda J, Nomura K, Inaba T, Shimazaki C, Horiike S, Taniwaki M.
  • Journal Title: Int J Hematol 89 Pages: 352-358
  • NAID: 10025160884
• [Journal Article] NUP98-NSD3 fusion gene in radiation-associated myelodysplastic syndrome with t(8;11)(p11;p15) and expression pattern of the NSD family genes. (2009)
  • Author(s): Taketani T, Taki T, Nakamura H, Taniwaki M, Masuda J, Hayashi Y.
  • Journal Title: Cancer Genet Cytogenet 190 Pages: 108-112
• [Journal Article] Transient abnormal myelopoiesis in a newborn with Down syndrome followed by an acute myeloid leukemia : identification of the same structural chromosomal abnormality at both stage of TAM and leukemia. (2009)
  • Author(s): Kitoh T, Taki T, Hayashi Y, Nakamura K, Irino T, Osaka M.
  • Journal Title: Cancer Genet Cytogenet 188 Pages: 99-102
• [Journal Article] MLL fusion protein collaborates with Raf to induce acute leukemia through aberrant Hox expression and Raf activation. (2009)
  • Author(s): Ono R, et al.
  • Journal Title: Leukemia 23 Pages: 2197-2209
  • Peer Reviewed
• [Journal Article] Immunologically silent cancer clone transmission from mother to offspring. (2009)
  • Author(s): Isoda T, et al.
  • Journal Title: Proc Natl Acad Sci USA 106 Pages: 17882-17885
  • Peer Reviewed
• [Journal Article] Alteration of enhancer of polycomb 1 at 10p11.2 is one of the geneticevents for developing adult T-cell leukemia/lymphoma. (2009)
  • Author(s): Nakahata S, et al.
  • Journal Title: Genes Chromosomes Cancer 48 Pages: 768-776
  • Peer Reviewed
• [Journal Article] Acute megakaryoblastic leukemia in a child with the MLL-AF4 fusiongene. (2009)
  • Author(s): Takita J, et al.
  • Journal Title: Eur J Haematol 83 Pages: 149-153
  • Peer Reviewed
• [Journal Article] Juvenile myelomonocytic leukaemia with t(7 ; 11)(pl5 ; p15) and NUP98-HOXA11 fusion. (2009)
  • Author(s): Mizoguchi Y, et al.
  • Journal Title: Am J Hematol 84 Pages: 295-297
  • Peer Reviewed
• [Journal Article] FBXW7 and NOTCH1 mutations in childhood T-cell acute lymphoblastic leukemia and T cell non-Hodgkin's lymphoma. (2009)
  • Author(s): Park M, et al.
  • Journal Title: Br J Haematol 145 Pages: 198-206
  • Peer Reviewed
• [Journal Article] Monosomies 7p and 12p and FLT3 internal tandem duplication : possible markers for diagnosis of T/myeloid biphenotypic acute leukemia and its clonal evolution. (2009)
  • Author(s): Matsumoto Y, et al.
  • Journal Title: Int J Hematol 89 Pages: 352-358
  • Peer Reviewed
• [Journal Article] Complex t(1;22;11)(q44;q13;q23) translocation causing MLL-p300 fusion gene in therapy-related acute myeloid leukemia. (2008)
  • Author(s): Ohnishi H, Taki T, Yoshino H, Takita J, Ida K, Ishii M, Nishida K, Hayashi Y, Taniwaki M, Bessho F, Watanabe T.
  • Journal Title: Eur J Haematol 81 Pages: 475-480
• [Journal Article] Fusion of OTT to BSAC results in aberrant up-regulation of transcriptional activity. (2008)
  • Author(s): Sawada T, Nishiyama C, Kishi T, Sasazuki T, Komazawa-Sakon S, Xue X, Piao JH, Ogata H, Nakayama J, Taki T, Hayashi Y, Watanabe M, Yagita H, Okumura K, Nakano H.
  • Journal Title: J Biol Chem 283 Pages: 26820-26828
• [Journal Article] HLXB9-ETV6 fusion gene in an acute megakaryoblastic leukemia patient and expression of the HLXB9 gene in leukemia and normal B cell lines. (2008)
  • Author(s): Taketani T, Taki T, Sako M, Ishii T, Hayashi Y.
  • Journal Title: Cancer Genet Cytogenet 186 Pages: 115-119
• [Journal Article] Novel gain-of-function mutation in the extracellular domalular domaar domar domaar domaa domar doma domar domar doma doma doma doma doma domaadomaadomaa domaaof the PDGFRA gene in infant acute lymphoblastic leukemia with t(4;11)(q21;q23). (2008)
  • Author(s): Hiwatari M, Ono R, Taki T, Hishiya A, Ishii E, Kitamura T, Hayashi Y, Nosaka T.
  • Journal Title: Leukemia 22 Pages: 2279-2280
• [Journal Article] Down-regulation of TCF8 is involved in the leukemogenesis of adult-T cell leukemia/lymphoma. (2008)
  • Author(s): Hidaka T, Nakahata S, Hatakeyama K, Hamasaki M, Yamashita K, Kohno T, Arai Y, Taki T, Nishida K, Okayama A, Asada Y, Yamaguchi R, Tsubouchi H, Yokota J, Taniwaki M, Higashi Y, Morishita K.
  • Journal Title: Blood 112 Pages: 383-393
• [Journal Article] Fully automated and super-rapid system for the detection of JAK2V617F mutation. (2008)
  • Author(s): Tanaka R, Kuroda J, Stevenson W, Ashihara E, Ishikawa T, Taki T, Kobayashi Y, Kamitsuji Y, Kawata E, Takeuchi M, Murotani Y, Yokota A, Hirai M, Majima S, Taniwaki M, Maekawa T, Kimura S.
  • Journal Title: Leuk Res 32 Pages: 1462-1467
• [Journal Article] Identification of the novel AML1 fusion partner gene, LAF4, a fusion partner of MLL, in childhood T-cell acute lymphoblastic leukemia with t(2;21)(q11;q22) by bubble PCR method for cDNA. (2008)
  • Author(s): Chinen Y, Taki T, Nishida K, Shimizu D, Okuda T, Yoshida N, Kobayashi C, Koike K, Tsuchida M, Hayashi Y, Taniwaki M.
  • Journal Title: Oncogene 27 Pages: 2249-2256
• [Journal Article] Tandem duplications of MLL and FLT3 are correlated with poor prognoses in pediatric acute myeloid leukemia : a study of the Japanese childhood AML Cooperative Study Group. (2008)
  • Author(s): Shimada A, Taki T, Tabuchi K, Taketani T, Hanada R, Tawa A, Tsuchida M, Horibe K, Tsukimoto I, Hayashi Y.
  • Journal Title: Pediatr Blood Cancer 50 Pages: 264-269
• [Journal Article] Good prognosis for 7-year-old Down syndrome patient with acute myeloid leukemia (FAB-M2), lacking mutations in GATA1, FLT3, MLL, NRAS and AML1 genes. (2008)
  • Author(s): Kawamura M, Kaku H, Taketani T, Taki T, Shimada A, Hayashi Y.
  • Journal Title: Cancer Genet Cytogenet 180 Pages: 74-78
• [Journal Article] Identification of the novel AML1 fusion partner gene, LAF4, a fusion partner of MLL, in childhood T-cell acute lymphoblastic leukemia with t(2 ; 21)(q11 ; q22)by bubble PCR method for cDNA. (2008)
  • Author(s): Chinen Y
  • Journal Title: Oncogene 27 Pages: 2249-2256
  • Peer Reviewed
• [Journal Article] Down-regulation of TCF8 is involved in the leukemogenesis of adult-T cell leukemia/iymphoma. (2008)
  • Author(s): Hidaka T
  • Journal Title: Blood 112 Pages: 383-393
  • Peer Reviewed
• [Journal Article] Novel gain-of-function mutation in the extracellular domain of the PDGFRA gene in infant acute lymphoblastic leukemia with t(4 ; 11)(q21 ; q23). (2008)
  • Author(s): Hiwatari M
  • Journal Title: Leukemia 22 Pages: 2279-2280
  • Peer Reviewed
• [Presentation] 染色体異常・遺伝子異常からみた小児白血病-固形腫瘍、先天異常とのつながりまで- (2010)
  • Author(s): 滝智彦
  • Organizer: 第121回日本小児科学会 静岡地方会
  • Place of Presentation: 静岡商工会議所会館
  • Year and Date: 2010-03-06
• [Presentation] Identification of a novel IGHC-BACH2 fusion transcript in B-cell lymphoma/leukemia and the expression analysis of BACH2 related with other B-cell differentiation-associated genes in B-cell malignancies. (2010)
  • Author(s): Kobayashi S, Taki T, Chinen Y, Tsutsumi Y, Ohshiro M, Kobayashi T, Matsumoto Y, Kuroda J, Horiike S, Nishida K, Taniwaki M
  • Organizer: 52nd Annual Meeting of the American Society of Hematology
  • Place of Presentation: Orlando
• [Presentation] Identification and functional significance of novel type of structurally aberrant transcripts of DCC in B-cell malignancies. (2010)
  • Author(s): 名越久朗, 滝智彦, 黒田純也, 西田一弘, 後藤美奈子, 奥田恵子, 小林覚, 山本未央, 〓康彦, 古林勉, 松本洋典, 堀池重夫, 大槻剛巳, 谷脇雅史
  • Organizer: 52nd Annual Meeting of the American Society of Hematology
  • Place of Presentation: Orlando
• [Presentation] 多発性骨髄腫と悪性リンパ腫における癌関連遺伝子DCC の新しいタイプの異常転写産物の同定とその意義 (2010)
  • Author(s): 名越久朗, 滝智彦, 黒田純也, 西田一弘, 後藤美奈子, 奥田恵子, 小林覚, 山本未央, 〓康彦, 古林勉, 松本洋典, 堀池重夫, 大槻剛巳, 谷脇雅史
  • Organizer: 日本人類遺伝学会第55回大会
  • Place of Presentation: さいたま
• [Presentation] Expression of altered DCC gene in multiple myeloma and non-Hodgkin's lymphoma. (2010)
  • Author(s): 名越久朗, 滝智彦, 黒田純也, 西田一弘, 後藤美奈子, 奥田恵子, 小林覚, 山本未央, 〓康彦, 古林勉, 松本洋典, 堀池重夫, 大槻剛巳, 谷脇雅史
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Novel PAX5-FOXP2 fusion gene in childhood B-cell precursor acute lymphoblastic leukemia. (2010)
  • Author(s): Tomohiko Taki, Natsumi Sakamoto, Junko Yamada, Yasuhiko Tsutsumi, Satoru Kobayashi, Hisao Nagoshi, Kousaku Matsubara, Kazuhiro Nishida, Masafumi Taniwaki
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜
• [Presentation] Identification of novel chimeric gene PVT1-NBEA(BCL8B) in a multiple myeloma cell line with t(8;13)(q24;q13) (2010)
  • Author(s): Hisao Nagoshi, Tomohiko Taki, Ichiro Hanamura, Junya Kuroda, Kazuhiro Nishida, Satoru Kobayashi, Yasuhiko Tsutsumi, Tsutomu Kobayashi, Yosuke Matsumoto, Shigeo Horiike, Takemi Otsuki, Masakazu Nitta, Msafumi Taniwaki
  • Organizer: 第69回日本癌学会総会
  • Place of Presentation: 大阪
• [Presentation] Alterations of DCC gene in B-cell malignancies. (2009)
  • Author(s): 名越久朗, 滝智彦, 西田一弘, 後藤美奈子, 小林覚, 〓康彦, 松本洋典, 黒田純也, 堀池重夫, 大槻剛巳, 谷脇雅史
  • Organizer: 51st Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] High frequencies of simultaneous FLT3-ITD, WT1, and KIT mutations in NUP98-related hematologic malignancies. (2009)
  • Author(s): Taketani T, Taki T, Fukuda S, Onishi C, Abe M, Yamaguchi S, Hayashi Y.
  • Organizer: 51st Annual Meeting of the American Society of Hematology
  • Place of Presentation: New Orleans
• [Presentation] Alteration of DCC gene in multiple myeloma cell lines. (2009)
  • Author(s): Hisao Nagoshi, Tomohiko Taki, Kazuhiro Nishida, Minako Goto, Satoru Kobayashi, Yasuhiko Tsutsumi, Yosuke Matsumoto, Junya Kuroda, Shigeo Horiike, Takemi Otsuki, Masafumi Taniwaki
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] Clinical significance of somatic mutations in hematological malignancies with NUP98-fusion genes. (2009)
  • Author(s): Takeshi Taketani, Tomohiko Taki, Seiji Fukuda, Seiji Yamaguchi, Yasuhide Hayashi
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] Novel fusion of the immunoglobulin heavy chain with BACH2 gene in diffuse large B-cell lymphoma. (2009)
  • Author(s): Satoru Kobayashi, Tomohiko Taki, Kazuhiro Nishida, Muneo Ohshiro, Kayoko Kurita, Natsumi Sakamoto, Yoshiaki Chinen, Hisao Nagoshi, Yasuhiko Tsutsumi, Yosuke Matsumoto, Junya Kuroda, Shigeo Horiike, Masafumi Taniwaki
  • Organizer: 第71回日本血液学会学術集会
  • Place of Presentation: 京都
• [Presentation] 未知のキメラ転写産物の同定を目的としたcDNA バブルPCR 法の確立 (2009)
  • Author(s): 滝智彦, 知念良顕, 小林覚, 名越久朗, 〓康彦, 栗田佳代子, 坂元奈津美, 西田一弘, 谷脇雅史
  • Organizer: 日本人類遺伝学会第54回大会
  • Place of Presentation: 東京
• [Presentation] AML97登録症例における染色体異常と臨床像 (2008)
  • Author(s): 滝 智彦
  • Organizer: 第70回日本血液学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-10-10
• [Presentation] Cloning of novel fusion partner gene of MOZ in therapy-related AML with t(8;19)(p11;p13)by bubble PCR method for cDNA (2008)
  • Author(s): Yoshiaki Chinen, Tomohiko Taki, Mihiko Yamashita, Yasuhiko Tsutsumi, Tsutomu Kobayashi, Satoru Kobayashi, Yosuke Matsumoto, Junya Kuroda, Sigeo Horiike, Kazuhiro Nishida, Hirofumi Ohno, Naokuni Uike, Masafumi Taniwaki
  • Organizer: 第67回日本癌学会総会
  • Place of Presentation: 名古屋
• [Book] 造血器腫瘍アトラス (2009)
  • Author(s): 滝 智彦
  • Publisher: 日本医事新報社