Investigation of Molecular Mechanisms of Apoptosis using gene knock-out leukemic cell lines
Project/Area Number |
20591261
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | Kitasato University |
Principal Investigator |
MIYASHITA Toshiyuki Kitasato University, 医学部, 教授 (60174182)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | アポトーシス / グルココルチコイド / RCAN1 / 白血病 / 相同組換え / カスパーゼ |
Research Abstract |
In order to investigate the molecular mechanism of how glucocorticoid (GC) induces apoptosis in certain leukemic cells, we established RCAN1 gene knock-out Nalm6 cells. RCAN1 is one of the target genes of GC and Nalm6 is a human leukemic cell line that undergoes apoptosis upon the treatment with GC. RCAN1 -/- cells were markedly resistant to GC compared with wild-type cells. On the contrary, overexpression of RCAN1 resulted in enhanced apoptosis by GC. These resultsdemonstrate that RCAN1 is a mediator for GC-induced apoptosis in leukemic cells.
|
Report
(4 results)
Research Products
(43 results)
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] EYA4, Deleted in a case with middle interhemispheric variant of holoprosencephaly, interacts with SIX3 both physically and functionally.2009
Author(s)
Abe Y., Oka A., Mizuguchi M., Igarashi T., Ishikawa S., Aburatani H., Yokoyama S., Asahara H., Nagao K., Yamada M., Miyashita T.
-
Journal Title
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Patched1 haploinsufficiency increases adult bone mass and modulates Gli3 repressor activity.2008
Author(s)
Ohba S., Kawaguchi H., Kugimiya F., Ogasawara T., Kawamura N., Saito T., Ikeda T., Fujii K., Miyajima T., Kuramochi A., Miyashita T., Oda H., Nakamura K., Takato T., Chung, U.I.
-
Journal Title
Dev.Cell 14
Pages: 689-699
Related Report
Peer Reviewed
-
-
-
-
-
-
-
[Presentation]2010
Author(s)
宮下俊之,遠藤真美子,齋藤加代子,杉田克生,藤井克則
Organizer
第16回日本家族性腫瘍学会学術集会
Place of Presentation
新潟
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-