Analysis of phosphorylation cascade of molecules expressed in podocyte as the pathophysiological basis of kidney diseases
| Project/Area Number |
20591271
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Toho University (2009-2010)
The University of Tokyo (2008) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
IGARASHI Takashi 東京大学, 医学部附属病院, 教授 (70151256)
MIURA Kenichiro 東京大学, 医学部附属病院, 助教 (70408483)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ネフローゼ症候群 / 巣状糸球体硬化症 / ポドサイト / リン酸化 / nepnrin / neph1 / TRPC6 / MYH9 / nephrin / phospholipase C(PLC)-γ1 / 細胞内Ca濃度 / チロシンリン酸化 / シグナル伝達 / NMMHC-IIA / podocyte / FSGS / 細胞内シグナル伝達 / PLC / 細胞内Ca |
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| Research Abstract |
In this study, we demonstrated and reported the following findings.
1) Phosphorylation of adhesion molecules of podocyte, such as neph1 is related to the development of proteinuria.
2) Nephrin binds to PLC-γ1 through its phosphorylation and it evokes the increase of intracellular calcium concentration([Ca2^+] i).
3) In adiition, we demonstrated that nephrin regulated the activity of TRPC6 channel on podocyte cytoplasmic membrane, which also regulate([Ca2^<+]> i).
4) Finally, we analyzed inherited renal disease, i. e. Epstein syndrome, and revealed that its pathogenesis is FSGS, and myosin right chain IIA was decreased in this disease.
These findings are published in, JBC 2008, JBC 2009, KidneyInter 2010, and Mol Cell Biol 2011.
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Report
(4 results)
Research Products
(86 results)
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[Journal Article] Defective membrane expression of the Na^<+-> HCO_3-cotransporter NBCe1 is associated with familial migraine2010
Author(s)
Suzuki M, Paesschen WV, Stalmans I, Horita S, Yamada H, Bergmans BA, Legius E, Riant F, Jonghe PD, Li Y, Sekine T, Igarashi T, Fujimoto I, Mikoshiba K, Shimadzu M, Shiohara M, Braverman N, Al-Gazali L, Fujita T, and Seki G
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Journal Title
PNAS
Volume: 107(36)
Pages: 15963-15968
Related Report
Peer Reviewed
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[Journal Article] Four cases of postrenal renal failure induced by renal stone associated with rotavirus infection2010
Author(s)
Morita T, Ashida A, Fujieda M, Hayashi A, Maeda A, Ohta K, Shimizu M, Sekine T, Igarashi T, Tamai H, Wakiguchi H
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Journal Title
Clin Nephrol
Volume: 73(5)
Pages: 398-402
Related Report
Peer Reviewed
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[Journal Article] A Case Report : Prenatal Intervention for Severe Anterior Urethral Valve2010
Author(s)
Mizuno Y, Tsuchida S, Kakiuchi S, Ishiguro A, Goishi K, Kamei Y, Kanamori Y, Yamazaki Y, Sekine T, Igarashi T.
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Journal Title
Pediatrics International
Volume: Vol.52, No.6
Related Report
Peer Reviewed
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[Journal Article] Patients with Epstein-Fechtner syndromes owing to MYH9 R702 mutations develop progressive proteinuric renal disease2010
Author(s)
Sekine T, Konno M, Sasaki S, Moritani S, Miura T, Wong WS, Nishio H, Nishiguchi T, Ohuchi MY, Tsuchiya S, Matsuyama T, Kanegane H, Ida K, Miura K, Harita Y, Hattori M, Horita S, Igarashi T, Saito H, Kunishima S.
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Journal Title
Kidney Int
Volume: 78(2)
Pages: 207-214
Related Report
Peer Reviewed
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[Presentation] 小児腎臓病学とは2010
Author(s)
関根孝司
Organizer
日本小児腎臓病学会主催若手小児腎臓医セミナー
Place of Presentation
筑波大学(特別講演)
Year and Date
2010-10-31
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