The role of Hic-5 in intractable glomerulonephritis in children

• Project/Area Number: 20591277
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: The University of Tokushima
• Principal Investigator: KONDO Shuji The University of Tokushima, ヘルスバイオサイエンス研究部 (00380080)
• Co-Investigator(Kenkyū-buntansha): KAGAMI Shoji 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (00224337)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 小児腎 / 泌尿器学 / 細胞・組織 / シグナル伝達 / 細胞接着分子 / 糸球体腎炎 / 病理学

Research Abstract

The purpose of this research is to clarify the expression of Hic-5 in nephritic glomeruli and how Hic-5 affects mesangial cell (MC) function. Immunohistochemistry showed that the expression of Hic-5 was increased in MC in human and rat mesangial proliferative glomerulonephritis (GN). Hic-5 expression was significantly correlated with extracellular matrix (ECM) accumulation and glomerular cell number in patients and GN rats. TGF- up-regulated Hic-5 protein levels by human cultured MCs. Knockdown of Hic-5 by siRNA reduced ECM remodeling in collagen gels. Thus, our findings suggest that Hic-5 is involved in the development of glomerulonephritis. Controlling Hic-5 activity might be a therapy strategy for GN.

Research Products

• [Journal Article] Angiotensin II type I receptor blockade suppresses glomerular RAS activation, oxidative stress, and glomerular injury in crescentic glomerulonephritis. (2011)
  • Author(s): Kinoshita Y, Kondo S, Urushihara M, Suga K, Matsuura S, Takamatsu M, Shimizu M, Nishiyama A, Kawachi H, Kagami S.
  • Journal Title: Translational Res (in press)
  • Peer Reviewed
• [Journal Article] A case of a 6-year-old girl with anti-neutrophil cytoplasmic autoantibody-negative pauci-immune crescentic glomerulonephritis. (2011)
  • Author(s): Shimizu M, Sekiguchi T, Kishi N, Goji A, Takahashi T, Kozan H, Sakaguchi Z, Kinoshita Y, Matsuura S, Suga K, Urushihara M, Kondo S, Kagami S, Ohara K.
  • Journal Title: Clin Exp Nephrol. (in press)
  • NAID: 10029572064
  • Peer Reviewed
• [Journal Article] Progress of pediatric nephrology. (2011)
  • Author(s): Kondo S, Urushihara M, Kagami S.
  • Journal Title: Nippon Jinzo Gakkai Shi. 53(1) Pages: 10-4
  • NAID: 10029735752
  • Peer Reviewed
• [Journal Article] ERK5 activation enhances mesangial cell viability and collagen matrix accumulation in rat progressive glomerulonephritis. (2010)
  • Author(s): Urushihara M, Takamatsu M, Shimizu M, Kondo S, Kinoshita Y, Suga K, Kitamura A, Matsuura S, Yoshizumi M, Tamaki T, Kawachi H, Kagami S.
  • Journal Title: Am J Physiol Renal Physiol. 298
  • Peer Reviewed
• [Journal Article] Urinary angiotensinogen accurately reflects intrarenal Renin-Angiotensin system activity. (2010)
  • Author(s): Urushihara M, Kondo S, Kagami S, Kobori H.
  • Journal Title: Am J Nephrol. 31 Pages: 318-325
  • Peer Reviewed
• [Journal Article] A case of dense deposit disease associated with a group A streptococcal infection without the involvement of C3NeF or complement factor H deficiency. (2010)
  • Author(s): Suga K, Kondo S, Matsuura S, Kinoshita Y, Kitano E, Hatanaka M, Kitamura H, Hidaka Y, Oda T, Kagami S.
  • Journal Title: Pediatr Nephrol. 25 Pages: 1547-1550
  • Peer Reviewed
• [Journal Article] A case of dense deposit disease associated with a group A streptococcal infection without the involvement of C3NeF or complement factor H deficiency. (2010)
  • Author(s): Suga K, Kondo S, et al
  • Journal Title: Pediatric Nephrology Volume: 25 Pages: 1547-1550
  • Peer Reviewed
• [Journal Article] Urinary Angiotensinogen Accurately Reflects Intrarenal Renin-Angiotensin System Activity. (2010)
  • Author(s): Urushihara M, Kondo S, et al
  • Journal Title: American Journal of Nephrology Volume: 31 Pages: 318-325
  • Peer Reviewed
• [Journal Article] ERK5 activation enhances mesangial cell viability and collagen matrix accumulation in rat progressive glomerulonephritis (2010)
  • Author(s): Urushihara M, Takamatsu M, et al
  • Journal Title: American Journal of Physiology Renal Physiology Volume: 298
  • Peer Reviewed
• [Journal Article] ACE阻害薬、ARB(アンジオテンシンII拮抗薬) (2010)
  • Author(s): 近藤秀治, 香美祥二
  • Journal Title: 小児科臨床ピクシス Volume: 22 Pages: 192-194
• [Journal Article] 腎発生におけるbc1-2の役割 (2010)
  • Author(s): 近藤秀治, 他
  • Journal Title: 発達腎研究会誌 Volume: 18 Pages: 6-9
  • Peer Reviewed
• [Journal Article] Low-density lipoprotein apheresis in a pediatric patient with refractory nephrotic syndrome due to focal segmental glomerulosclerosis. (2009)
  • Author(s): Oto J, Suga K, Matsuura S, Kondo S, Ohnishi Y, Inui D, Imanaka H, Kagami S, Nishimura M.
  • Journal Title: J Anesth. 23 Pages: 284-287
  • NAID: 10025382391
  • Peer Reviewed
• [Journal Article] Low-density lipoprotein apheresis in a pediatric patient with refractory nephritic syndrome due to focal segmental glomerulosclerosis. (2009)
  • Author(s): Oto J, Suga K, et al
  • Journal Title: Journal of Anesthesia 23 Pages: 284-287
  • Peer Reviewed
• [Journal Article] ラット腎発生でのHic-5、focal adhesion kinase、パキシリンの発現 (2009)
  • Author(s): 近藤秀治, 他
  • Journal Title: 発達腎研究会誌 17 Pages: 30-33
• [Journal Article] bcl-2欠損マウスでみられる腎病変は尿管芽由来上皮のbcl-2の発現により改善する (2009)
  • Author(s): 近藤秀治, 他
  • Journal Title: 日本小児腎臓病学会雑誌 22 Pages: 136-140
  • NAID: 10026411802
  • Peer Reviewed
• [Journal Article] Rescue of renal hypoplasia and cystic dysplasia in Bcl-2-/- mice expressing Bcl-2 in ureteric bud derived epithelia. (2008)
  • Author(s): Kondo S, Oakes MG, Sorenson CM.
  • Journal Title: Dev Dyn. 237 Pages: 2450-2459
  • Peer Reviewed
• [Journal Article] Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2. (2008)
  • Author(s): Kondo S, Tang Y, Scheef EA, Sheibani N, Sorenson CM.
  • Journal Title: Am J Physiol Cell Physiol. 294
  • Peer Reviewed
• [Journal Article] Dietary doses of nitrite restore circulating nitric oxide level and improve renal injury in L-NAME-induced hypertensive rats. (2008)
  • Author(s): Kanematsu Y, Yamaguchi K, Ohnishi H, Motobayashi Y, Ishizawa K, Izawa Y, Kawazoe K, Kondo S, Kagami S, Tomita S, Tsuchiya K, Tamaki T.
  • Journal Title: Am J Physiol Renal Physiol. 295
  • Peer Reviewed
• [Journal Article] Glomerular angiotensinogen protein is enhanced in pediatric IgA nephropathy. (2008)
  • Author(s): Takamatsu M, Urushihara M, Kondo S, Shimizu M, Morioka T, Oite T, Kobori H, Kagami S.
  • Journal Title: Pediatr Nephrol. 23 Pages: 1257-1267
  • Peer Reviewed
• [Journal Article] Glomerular Angiotensinogen Protein is Enhanced in Pediatric IgA Nephropathy. (2008)
  • Author(s): Takamatsu M, et al.
  • Journal Title: Pediatric Nephrology 23 Pages: 1257-1267
  • Peer Reviewed
• [Journal Article] Dietary doses of nitrite restore the circulating nitric oxide level and improve renal injury in L-NAME-induced hypertensive rats. (2008)
  • Author(s): Kanematsu Y, et al.
  • Journal Title: Am J Physiol renal physiol 295
  • Peer Reviewed
• [Journal Article] マウス腎血管形成におけるPECAM-1の役割 (2008)
  • Author(s): 近藤秀治, 他
  • Journal Title: 発達腎研究会誌 16 Pages: 43-46
• [Presentation] 路感染症について (2010)
  • Author(s): 近藤秀治
  • Organizer: 第66回はぐくみ支援セミナー
  • Place of Presentation: 徳島市、徳島県総合福祉センター
  • Year and Date: 2010-12-16
• [Presentation] 慢性甲状腺炎と開放偶角緑内障を合併した膜性腎症の一例 (2010)
  • Author(s): 松浦里, 他
  • Organizer: 第27回中国四国小児腎臓病学会
  • Place of Presentation: 広島市、広島国際会議場
  • Year and Date: 2010-10-10
• [Presentation] 乳児期に発症した全身性エリテマトーデス(SLE)の一例 (2010)
  • Author(s): 岸夏子, 他
  • Organizer: 第40回日本腎臓学会西部学術大会
  • Place of Presentation: 広島市、広島国際会議場
  • Year and Date: 2010-10-08
• [Presentation] NADPH oxidase由来の活性酸素は尿管芽分岐とネフロン形成に関与する (2010)
  • Author(s): 松浦里, 他
  • Organizer: 第19回発達腎研究会
  • Place of Presentation: 東京、慶応大学医学部
  • Year and Date: 2010-09-18
• [Presentation] 学校検尿(蛋白・潜血)システムについて-21年度検診結果および22年度検診について- (2010)
  • Author(s): 近藤秀治
  • Organizer: 平成21年度 学校検尿検診・小児肥満の健康管理システム二次協力医療機関研修会
  • Place of Presentation: 徳島市、徳島県医師会館
  • Year and Date: 2010-04-02
• [Presentation] Expression and localization of focal adhesion proteins in the developing rat kidney. (2010)
  • Author(s): Matsuura S, Kondo S, Suga K, Kinoshita Y, Tamaki T, Kagami S
  • Organizer: The 15th Congress of the International Pediatric Nephrology Association (IPNA)
• [Presentation] Candesartan Suppresses Glomerular Renin-Angiotensin System (RAS) Activation, Oxidative Stress and Progressive Glomerular Injury in Rat Anti-GBM GN (2010)
  • Author(s): Kinoshita Y, Kondo S, Suga K, Matsuura S, Urushihara M, Kagami S
  • Organizer: The 15th Congress of the International Pediatric Nephrology Association (IPNA)
• [Presentation] Enhanced expression of Hic-5 is involved in the development of human and rat mesangioproliferative glomerulonephritis. (2010)
  • Author(s): Kondo S, Suga K, Matsuura S, Kinoshita Y, Urushihara M, Tamaki T, Kagami S
  • Organizer: ISN-Nexus Kyoto Symposium
  • Place of Presentation: Kyoto
• [Presentation] Expression and localization of focal adhesion proteins in the developing rat kidney. (2010)
  • Author(s): Matsuura S, et al
  • Organizer: The 15th Congress of the International Pediatric Nephrology Association(IPNA)
  • Place of Presentation: New York, USA
• [Presentation] Activation, Oxidative Stress and Progressive Glomerular Injury in Rat Anti-GBM GN (2010)
  • Author(s): Kinoshita Y, et al
  • Organizer: The 15th Congress of the International Pediatric Nephrology Association(IPNA)
  • Place of Presentation: New York, USA
• [Presentation] 後腹膜鏡下リンパ管遮断術を行った特発性乳び尿の1例 (2010)
  • Author(s): 松浦里, 他
  • Organizer: 第113回日本小児科学会学術集会
  • Place of Presentation: 岩手市、盛岡市民文化ホール
• [Presentation] 乳児期に発症した全身性エリテマトーデス(SLE)の一例 (2010)
  • Author(s): 岸夏子, 他
  • Organizer: 113回日本小児科学会学術集会
  • Place of Presentation: 岩手市、盛岡市民文化ホール
• [Presentation] ラット腎発生における細胞接着斑蛋白の発現 (2010)
  • Author(s): 松浦里, 他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸市、神戸国際会議場
• [Presentation] 半月体形成性糸球体腎炎におけるアンジオテンシンII(AngII)の役割 (2010)
  • Author(s): 木下ゆき子, 他
  • Organizer: 第53回日本腎臓学会学術総会
  • Place of Presentation: 神戸市、神戸国際会議場
• [Presentation] ラット腎発生における細胞接着斑蛋白の発現 (2010)
  • Author(s): 松浦里, 他
  • Organizer: 第45回日本小児腎臓病学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
• [Presentation] 半月体形成性糸球体腎炎におけるアンジオテンシンII(AngII)の役割 (2010)
  • Author(s): 木下ゆき子, 他
  • Organizer: 第45回日本小児腎臓病学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
• [Presentation] NPHP4遺伝子異常を認めた若年性ネフロン癆の母子例 (2010)
  • Author(s): 清水真樹, 他
  • Organizer: 第45回日本小児腎臓病学会学術集会
  • Place of Presentation: 大阪市、大阪国際会議場
• [Presentation] Expression and localization of proteins in focal adhesion in the developing rat kidney (2009)
  • Author(s): Shuji Kondo, et al
  • Organizer: American Society of Nephrology
  • Place of Presentation: サンディエゴ
  • Year and Date: 2009-10-29
• [Presentation] 後腹膜鏡下リンパ遮断術を行った特発性乳び尿の1例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第26回中国四国小児腎臓病学会
  • Place of Presentation: 高知市
  • Year and Date: 2009-10-25
• [Presentation] 学校検尿を契機に発見されたMPO-ANCA関連半月体形成性腎炎の2例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第39回日本腎臓学会西部学術大会
  • Place of Presentation: 和歌山市
  • Year and Date: 2009-10-16
• [Presentation] 結節性多発動脈炎の14歳女児例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第19回日本小児リウマチ学会総会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-04
• [Presentation] 頚部リンパ節炎で発症し診断に苦慮した全身性エリテマトーデスの1例 (2009)
  • Author(s): 須賀健一, 他
  • Organizer: 第19回日本小児リウマチ学会総会学術集会
  • Place of Presentation: 京都市
  • Year and Date: 2009-10-03
• [Presentation] bcl-2-/-マウスの腎低形成や嚢胞腎は尿管芽由来上皮へのbcl-2の発現により改善する (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第18回発達腎研究会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-09-06
• [Presentation] 徳島県の学校検尿検診システムの現状と徳島大学病院小児科での腎生検診断と治療の評価 (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第239回徳島医学会
  • Place of Presentation: 徳島市
  • Year and Date: 2009-08-02
• [Presentation] ヒト糸球体腎炎におけるHydrogen peroxide-inducible clone 5 (Hic-5)の発現に関する検討 (2009)
  • Author(s): 須賀健一, 他
  • Organizer: 第44回日本小児腎臓病学会
  • Place of Presentation: 東京都
  • Year and Date: 2009-06-27
• [Presentation] Bcl-2-/-でみられる腎病変は尿管芽由来上皮のbcl-2の発現により改善する (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第44回日本小児腎臓病学会
  • Place of Presentation: 東京都
  • Year and Date: 2009-06-27
• [Presentation] ラット進行性糸球体腎炎進展におけるHic-5の発現 (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第44回日本小児腎臓病学会
  • Place of Presentation: 東京都
  • Year and Date: 2009-06-27
• [Presentation] ヒト糸球体腎炎におけHydrogen peroxide-inducible clone 5 (Hic-5)の発現に関する検討 (2009)
  • Author(s): 須賀健一, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-06-04
• [Presentation] Bcl-2の尿管芽由来上皮への発現はbcl-2-/-マウスでみられる腎病変を改善する (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-06-04
• [Presentation] ラット進行性糸球体腎炎におけるHic-5の発現の検討 (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第52回日本腎臓学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-06-04
• [Presentation] 同一患者に紫斑病腎炎とIgA腎症がみとめられた3例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第112回日本小児科学会学術集会
  • Place of Presentation: 奈良市
  • Year and Date: 2009-04-19
• [Presentation] Bcl-2は未熟児網膜症における血管内皮細胞の遊走や管腔形成に関与する (2009)
  • Author(s): 近藤秀治, 他
  • Organizer: 第112回日本小児科学会学術集会
  • Place of Presentation: 奈良市
  • Year and Date: 2009-04-18
• [Presentation] 紫斑病性腎炎とIgA腎症を同一患者に発症した3症例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第3回四国腎疾患談話会
  • Place of Presentation: 高松市
  • Year and Date: 2009-03-07
• [Presentation] 移植腎に再発した巣状糸球体硬化症の1例 (2009)
  • Author(s): 松浦里, 他
  • Organizer: 第10回四国小児腎疾患研究会
  • Place of Presentation: 高知市
  • Year and Date: 2009-02-07
• [Presentation] Expression and localization of proteins in focal adhesion in the developing rat kidney. (2009)
  • Author(s): Kondo S, Suga K, Matuura S, Kinoshita Y, Tamaki T, Kagami S
  • Organizer: The American Society of Nephrology Renal Week 2009
  • Place of Presentation: San Diego, CA, USA
• [Presentation] Enhanced expression of Hydrogen peroxide-inducible clone-5 (Hic-5) on activated mesangial cells in human glomerulonephritis. (2008)
  • Author(s): Suka K, Kondo S, Matsuura S, Kinoshita Y, Urushihara M, Tamaki T, Kagami S
  • Organizer: The American Society of Nephrology Renal Week 2008
  • Place of Presentation: Philadelphia, Pennsylvania, USA
• [Presentation] The Expression of Hic-5 Is Involved in the Development of Progressive Glomerulonephritis in the Rat. (2008)
  • Author(s): Kondo S, Suka K, Matsuura S, Kinoshita Y, Urushihara M, Tamaki T, Kawachi H, Kagami S
  • Organizer: The American Society of Nephrology Renal Week 2008
  • Place of Presentation: Philadelphia, Pennsylvania, USA
• [Presentation] The Expression of Hic-5 Is Involved in the Development of Progressive Glomerulonephritis in the Rat. (2008)
  • Author(s): Kondo S, et al.
  • Organizer: The American Society of Nephrology Renal Week
  • Place of Presentation: Philadelphia
• [Presentation] Enhanced expression of Hydrogen peroxide-indueible clone-5 (Hic-5) on activated mesangial cells in human glomerulone phritis. (2008)
  • Author(s): Suka K, et al.
  • Organizer: The American Society of Nephrology Renal Week
  • Place of Presentation: Philadelphia
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