Molecular-epidemiological study of an emerging pathogen enteroaggregative Eschelichia coli and development of rapid diagnosis using immunochromatography.
| Project/Area Number |
20591282
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
NISHI Junichiro Kagoshima University, 医学部・歯学部附属病院, 講師 (40295241)
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| Co-Investigator(Kenkyū-buntansha) |
NOMURA Yuichi 鹿児島大学, 医歯学総合研究科, 准教授 (90237884)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 小児 / 下痢症 / 下痢原性大腸菌 / 腸管凝集性大腸菌 / EAEC / Aap / バイオフィルム / 付着線毛 / 迅速診断 / 乳幼児 / AggR / 酸耐性 |
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| Research Abstract |
We demonstrated that the incidence of typical enteroaggregative Esherichia coli (EAEC) among E.coli strains isolated from diarrheal children was 3.1% (76/2,417). The incidence of suface-layer protein Aap was 81.6% among typical EAEC strains, and its amino-acid sequences were well conserved. We successfully prepared anti-Aap antibodies using recombinant Aap protein, which can be applied for a rapid immunochromatographic detection system of EAEC.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms.2011
Author(s)
Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T.
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Journal Title
FEMS Microbiol Lett 317(2)
Pages: 109-116
Related Report
Peer Reviewed
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[Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms2010
Author(s)
Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T.
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Journal Title
FEMS Microbiol Lett.
Volume: 317(2)
Pages: 109-116
Related Report
Peer Reviewed
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[Journal Article] Correlation between meropenem and doripenem use density and the incidence of carbapenem-resistant Pseudomonas aeruginosa.2009
Author(s)
Shigemi A, Matsumoto K, Yaji K, Shimodozono Y, Takeda Y, Miyanohara H, Kawamura H, Orita M, Tokuda K, Nishi J, Yamada K.
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Journal Title
Int J Antimicrob Agents 34(6)
Pages: 589-91
Related Report
Peer Reviewed
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[Journal Article] Correlation between meropenem and doripenem use density and the incidence of carbapenem-resistant Pseudomonas aeruginosa. 20092009
Author(s)
Shigemi A, Matsumoto K, Yaji K, Shimodozono Y, Takeda Y, Miyanohara H, Kawamura H, Orita M, Tokuda K, Nishi J, Yamada K
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Journal Title
Int J Antimicrob Agents 34(6)
Pages: 589-591
Related Report
Peer Reviewed
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