Molecular-epidemiological study of an emerging pathogen enteroaggregative Eschelichia coli and development of rapid diagnosis using immunochromatography.

• Project/Area Number: 20591282
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Kagoshima University
• Principal Investigator: NISHI Junichiro Kagoshima University, 医学部・歯学部附属病院, 講師 (40295241)
• Co-Investigator(Kenkyū-buntansha): NOMURA Yuichi 鹿児島大学, 医歯学総合研究科, 准教授 (90237884)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 小児 / 下痢症 / 下痢原性大腸菌 / 腸管凝集性大腸菌 / EAEC / Aap / バイオフィルム / 付着線毛 / 迅速診断 / 乳幼児 / AggR / 酸耐性

Research Abstract

We demonstrated that the incidence of typical enteroaggregative Esherichia coli (EAEC) among E.coli strains isolated from diarrheal children was 3.1% (76/2,417). The incidence of suface-layer protein Aap was 81.6% among typical EAEC strains, and its amino-acid sequences were well conserved. We successfully prepared anti-Aap antibodies using recombinant Aap protein, which can be applied for a rapid immunochromatographic detection system of EAEC.

Research Products

• [Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms. (2011)
  • Author(s): Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T.
  • Journal Title: FEMS Microbiol Lett 317(2) Pages: 109-116
  • Peer Reviewed
• [Journal Article] レンサ球菌感染症 (2011)
  • Author(s): 西順一郎
  • Journal Title: 小児科臨床ピクシス Volume: 25 Pages: 158-163
• [Journal Article] Characterization of typical and atypical enteroaggregative Escherichia coli in Kagoshima, Japan : biofilm formation and acid resistance. (2010)
  • Author(s): Tokuda K, Nishi J, Imuta N, Fujiyama R, Kamenosono A, Manago K, Yoshifumi K.
  • Journal Title: Microb Immunol 54(6) Pages: 320-329
  • NAID: 10029545048
  • Peer Reviewed
• [Journal Article] Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae type b (Hib) strains in Japan before introduction of the Hib conjugate vaccine. (2010)
  • Author(s): Ueno K, Nishi J, Imuta N, Tokuda K, Kawano Y.
  • Journal Title: Microb Immunol 54(3) Pages: 160-163
  • NAID: 10029544305
  • Peer Reviewed
• [Journal Article] Characterization of typical and atypical enteroaggregative Escherichia coli in Kagoshima, Japan : biofilm formation and acid resistance (2010)
  • Author(s): Tokuda K, Nishi J, Imuta N, Fujiyama R, Kamenosono A, Manago K, Yoshifumi K.
  • Journal Title: Microb Immunol Volume: 54(6) Pages: 320-329
  • NAID: 10029545048
  • Peer Reviewed
• [Journal Article] Environmental mutagens may be implicated in the emergence of drug-resistant microorganisms (2010)
  • Author(s): Miyahara E, Nishie M, Takumi S, Miyanohara H, Nishi J, Yoshiie K, Oda H, Takeuchi M, Komatsu M, Aoyama K, Horiuchi M, Takeuchi T.
  • Journal Title: FEMS Microbiol Lett. Volume: 317(2) Pages: 109-116
  • Peer Reviewed
• [Journal Article] 整形外科患者より分離された鼻腔および感染創由来MRSA株のagr遺伝子型・バイオフィルム形成能の検討 (2010)
  • Author(s): 川村英樹, 西順一郎, 藺牟田直子, 徳田浩一, 宮之原弘晃, 河野嘉文, 小宮節郎
  • Journal Title: Bacterial Adherence & Biofilm Volume: 23 Pages: 19-21
  • Peer Reviewed
• [Journal Article] 鹿児島県におけるHibワクチンの前方視的安全性調査(中間報告) (2010)
  • Author(s): 西順一郎
  • Journal Title: 小児科 Volume: 51(10) Pages: 1307-1310
  • Peer Reviewed
• [Journal Article] 溶血性レンサ球菌感染症 (2010)
  • Author(s): 西順一郎
  • Journal Title: 小児内科 Volume: 42(増刊号) Pages: 352-355
• [Journal Article] Presence of multiple copies of capsulation loci in invasive Haemophilus influenzae typeb (Hib) strains in Japan before introduction of the Hib conjugate vaccine (2010)
  • Author(s): Ueno K, Nishi J, Imuta N, Tokuda K, Kawano Y
  • Journal Title: Microb Immunol 54(3) Pages: 160-163
  • NAID: 10029544305
  • Peer Reviewed
• [Journal Article] Correlation between meropenem and doripenem use density and the incidence of carbapenem-resistant Pseudomonas aeruginosa. (2009)
  • Author(s): Shigemi A, Matsumoto K, Yaji K, Shimodozono Y, Takeda Y, Miyanohara H, Kawamura H, Orita M, Tokuda K, Nishi J, Yamada K.
  • Journal Title: Int J Antimicrob Agents 34(6) Pages: 589-91
  • Peer Reviewed
• [Journal Article] An elevated value of high mobility group box 1 is a potential marker for poor response to high-dose of intravenous immunoglobulin treatment in patients with Kawasaki syndrome. (2009)
  • Author(s): Eguchi T, Nomura Y, Hashiguchi T, Masuda K, Arata M, Hazaki D, Ueno K, Nishi J, Kawano Y, Maruyama I.
  • Journal Title: Pediatr Infect Dis J 28(4) Pages: 339-341
  • Peer Reviewed
• [Journal Article] Correlation between meropenem and doripenem use density and the incidence of carbapenem-resistant Pseudomonas aeruginosa. 2009 (2009)
  • Author(s): Shigemi A, Matsumoto K, Yaji K, Shimodozono Y, Takeda Y, Miyanohara H, Kawamura H, Orita M, Tokuda K, Nishi J, Yamada K
  • Journal Title: Int J Antimicrob Agents 34(6) Pages: 589-591
  • Peer Reviewed
• [Journal Article] An elevated value of high mobility group box 1 is a potential marker for poor response to high-dose of intravenous immunoglobulin treatment in patients with Kawasaki syndrome (2009)
  • Author(s): Eguchi T, Nomura Y, Hashiguchi T, Masuda K, Arata M, Hazaki D, Ueno K, Nishi J, Kawano Y, Maruyama I
  • Journal Title: Pediatr Infect Dis J. 28(4) Pages: 339-341
  • Peer Reviewed
• [Journal Article] Descending necrotizing mediastinitis secondary to retropharyngeal abscess in a child. (2008)
  • Author(s): Yamasaki Y, Nishi J, Nishikawa T, Tatsumoto C, Kasano F, Sano N, Kawano Y, Kawakami K.
  • Journal Title: J Infect Chemother 14(3) Pages: 255-257
  • NAID: 10022595290
  • Peer Reviewed
• [Journal Article] The shf gene of Shigella flexneri homolog on the virulent plasmid pAA2 of enteroaggregative Escherichia coli 042 is required for firm biofilm formation. (2008)
  • Author(s): Fujiyama R, Nishi J, Imuta N, Tokuda K, Manago K, Kawano Y.
  • Journal Title: Current Microb 56(5) Pages: 474-480
  • Peer Reviewed
• [Journal Article] Proinflammatory cytokines in cerebrospinal fluid from patients with nontyphoidal Salmonella encephalopathy. (2008)
  • Author(s): Imamura M, Nishi J, Tamada I, Tenokuchi Y, Toyoshima M, Kawano Y.
  • Journal Title: Pediatr Infect Dis J 27(6) Pages: 558-559
  • Peer Reviewed
• [Journal Article] Proinflammatory cytokines in cerebrospinal fluid from patients with nontyphoidal Salmonella encephalopathy (2008)
  • Author(s): Imamura M, Nishi I, Tamada I., et al.
  • Journal Title: Pediatr Infect Dis J 27(6) Pages: 558-559
  • Peer Reviewed
• [Journal Article] Descending necrotizing mediastinitis secondary to retropharyngeal abscess in a child. (2008)
  • Author(s): Yamasaki Y, Nishi J, Nishikawa T, et al.
  • Journal Title: J Infect Chemother 14(3) Pages: 255-257
  • NAID: 10022595290
  • Peer Reviewed
• [Journal Article] Escherichia coli efflux pump TolC promotes aggregation of enteroaggregative E. coli 042 (2008)
  • Author(s): Imuta, N., Nishi J, et al.
  • Journal Title: Infect Immun 76 Pages: 1247-1256
  • Peer Reviewed
• [Journal Article] The shf gene of a Shigella flexneri homolog on the virulent plasmid pAA2 of enteroaggregative Escherichia coli 042 is required for firm biofilm formation (2008)
  • Author(s): Fujiyama R, Nishi J, et al.
  • Journal Title: Current Microb 56 Pages: 474-480
  • Peer Reviewed
• [Presentation] 小児下痢症患者から分離された大腸菌におけるcytolethal distending toxin (CDT)遺伝子保有状況 (2011)
  • Author(s): 藺牟田直子, 西順一郎
  • Organizer: 第7回日本小児消化管感染症研究会
  • Place of Presentation: 大阪
  • Year and Date: 2011-02-12
• [Presentation] 小児下痢症患者から分離された大腸菌におけるcytolethal distending toxin(CDT)遺伝子保有状況 (2011)
  • Author(s): 藺牟田直子, 西順一郎
  • Organizer: 第7回日本小児消化管感染症研究会
  • Place of Presentation: 大阪市
  • Year and Date: 2011-02-12
• [Presentation] 鹿児島県における小児細菌性髄膜炎の前方視的調査Hibワクチン安全性調査 (2010)
  • Author(s): 西順一郎, 徳田浩一, 藺牟田直子, 亀之園明, 河野嘉文
  • Organizer: 第42回日本小児感染症学会総会・学術集会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-11-28
• [Presentation] 腸管出血性大腸菌感染症の報告促進における課題-医師側要因に関するKAP調査- (2010)
  • Author(s): 徳田浩一, 西順一郎, 藺牟田直子, 河野嘉文
  • Organizer: 第42回日本小児感染症学会総会・学術集会
  • Place of Presentation: 仙台市
  • Year and Date: 2010-11-28
• [Presentation] 腸管出血性大腸菌感染症の報告促進における課題-医師側要因に関するKAP調査- (2010)
  • Author(s): 徳田浩一, 西順一郎, 藺牟田直子, 河野嘉文
  • Organizer: 第42回日本小児感染症学会総会・学術集会
  • Place of Presentation: 仙台
  • Year and Date: 2010-11-27
• [Presentation] 鹿児島県で分離された腸管凝集性大腸菌の病原遺伝子の系統解析 (2010)
  • Author(s): 藺牟田直子, 徳田浩一, 西順一郎
  • Organizer: 第80回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 松山
  • Year and Date: 2010-11-19
• [Presentation] 鹿児島県で分離された腸管凝集性大腸菌の病原遺伝子の系統解析 (2010)
  • Author(s): 藺牟田直子, 徳田浩一, 西順一郎
  • Organizer: 第80回日本感染症学会西日本地方会学術集会
  • Place of Presentation: 松山市
  • Year and Date: 2010-11-19
• [Presentation] 鹿児島市のヒブワクチン公費助成に伴う接種状況とその効果 (2010)
  • Author(s): 西順一郎
  • Organizer: 日本外来小児科学会第6回予防接種システム検討会
  • Place of Presentation: 京都市
  • Year and Date: 2010-09-23
• [Presentation] ワクチンによるHib髄膜炎予防効果 (2010)
  • Author(s): 西順一郎
  • Organizer: 第20回日本外来小児科学会年次集会
  • Place of Presentation: 福岡市
  • Year and Date: 2010-08-29
• [Presentation] 小児下痢症由来大腸菌における腸管凝集性大腸菌dispersin(Aap)の分布 (2010)
  • Author(s): 西順一郎, 藺牟田直子, 徳田浩一, 河野嘉文.
  • Organizer: 第14回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-07-22
• [Presentation] Genotyping and clinical background oftype IV SCCmec methicillin-resistant Staphylococcus aureus(MRSA)Strains isolated from orthopaedic patients in a university hospital in Japan. (2010)
  • Author(s): Kawamura H, Nishi J, Imuta N, Tokuda K, Kawano Y, Komiya S
  • Organizer: 110th General Meeting of American Society for Microbiology
  • Place of Presentation: San Diego, USA
  • Year and Date: 2010-05-24
• [Presentation] 鹿児島県におけるヒブワクチン安全性の前方視的調査(第2報) (2010)
  • Author(s): 西順一郎, 徳田浩一, 亀之園明, 河野嘉文
  • Organizer: 第113回日本小児科学会学術集会
  • Place of Presentation: 盛岡市
  • Year and Date: 2010-04-25
• [Presentation] 当院で分離された皮膚関連MRSA株のcollagen adhesin gene(cna)保有状況とbiofilm形成能 (2010)
  • Author(s): 藺牟田直子, 川村英樹, 徳田浩一, 宮之原弘晃, 西順一郎
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-06
• [Presentation] インフルエンザ菌b型(Hib)ワクチン (2010)
  • Author(s): 西順一郎
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-06
• [Presentation] 整形外科領域における手術部位検出菌と抗菌薬使用状況の関連 (2010)
  • Author(s): 川村英樹, 徳田浩一, 松元一明, 宮之原弘晃, 西順一郎
  • Organizer: 第84回日本感染症学会総会
  • Place of Presentation: 京都市
  • Year and Date: 2010-04-05
• [Presentation] 小児下痢症由来大腸菌における腸管凝集性大腸菌dispersin(Aap)の分布 (2010)
  • Author(s): 西順一郎, 藺牟田直子, 徳田浩一, 河野嘉文
  • Organizer: 第14回腸管出血性大腸菌感染症研究会
  • Place of Presentation: 宮崎
• [Presentation] 小児市中感染症-呼吸器感染症・髄膜炎・腸管感染症- (2009)
  • Author(s): 西順一郎
  • Organizer: 第19回南九州臨床微生物研究会
  • Place of Presentation: 人吉
  • Year and Date: 2009-10-25
• [Presentation] Genotyping and quantitative analysis of biofilm formation of methicillin-resistant Staphylococcus aureus(MRSA)strains from patients with orthopaedic infections (2009)
  • Author(s): Kawamura H, Nishi J, Imuta N, Tokuda K, Kawano Y, Komiya S.
  • Organizer: 109th General Meeting of American Society for Microbiology
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2009-05-26
• [Presentation] シンポジウム「小児消化器感染症の合併症」サルモネラ感染症の合併症 (2008)
  • Author(s): 西順一郎
  • Organizer: 第35回日本小児栄養消化器肝臓学会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-12
• [Presentation] ランチョンセミナー「微生物検査、臨床は何を求めているか?」医師が求める微生物検査」 (2008)
  • Author(s): 西順一郎
  • Organizer: 第43回九州医学検査学会
  • Place of Presentation: 鹿児島
  • Year and Date: 2008-10-11
• [Remarks] ホームページ等
• [Remarks] 鹿児島大学小児科HP 研究グループ 感染症
  • URL: http://www.kufm.kagoshima-u.ac.jp/~ped/
• [Remarks]
  • URL: http://www.kufm.kagoshima-u.ac.jp/~ped/
• [Remarks]
  • URL: http://www.kufm.kagoshima-u.ac.jp/~ped/group/kansen/kansenshou%20total.htm