Discovery of oxygen-sensitive protein -Proteomics approach-

Research Project

Project/Area Number	20591288
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Pediatrics
Research Institution	Nippon Medical School
Principal Investigator	KATSUBE Yasuhiro Nippon Medical School, 医学部, 准教授 (20246523)
Co-Investigator(Kenkyū-buntansha)	OGAWA Syunichi 日本医科大学, 医学部, 教授 (50194436) ASANO Takeshi 日本医科大学, 医学部, 准教授 (70277490)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000) Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000) Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords	酸素感受性 / 蛋白質 / プロテオミクス / プロテインチップ / 動脈管
Research Abstract	The aims of this study is to discover oxygen sensitive-protein using ductus arteriosus (DA) and peripheral pulmonary arteries (PPA) by proteomics analysis. Fetus rabbit DA and newborn rabbit PPA are used. Protein analysis is performed by SELDI TOF-MS analyzer and QSTAR Elite Hybrid LC-MS/MS system. Proteins highly expressed in DA compared to PPA are observed by SELDI TOF-MS system. Similarly, proteins highly expressed in mature fetus DA compared to immature fetus DA are observed by QSTAR Elite Hybrid LC-MS/MS system.