Discovery of oxygen-sensitive protein -Proteomics approach-
| Project/Area Number |
20591288
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Nippon Medical School |
|---|
Principal Investigator |
KATSUBE Yasuhiro Nippon Medical School, 医学部, 准教授 (20246523)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
OGAWA Syunichi 日本医科大学, 医学部, 教授 (50194436)
ASANO Takeshi 日本医科大学, 医学部, 准教授 (70277490)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Keywords | 酸素感受性 / 蛋白質 / プロテオミクス / プロテインチップ / 動脈管 |
|---|
| Research Abstract |
The aims of this study is to discover oxygen sensitive-protein using ductus arteriosus (DA) and peripheral pulmonary arteries (PPA) by proteomics analysis. Fetus rabbit DA and newborn rabbit PPA are used. Protein analysis is performed by SELDI TOF-MS analyzer and QSTAR Elite Hybrid LC-MS/MS system. Proteins highly expressed in DA compared to PPA are observed by SELDI TOF-MS system. Similarly, proteins highly expressed in mature fetus DA compared to immature fetus DA are observed by QSTAR Elite Hybrid LC-MS/MS system.
|
Report
(4 results)
Research Products
(2 results)
