Hepatocyte nuclear factor 4α promotes protein synthesis through the sodium-coupled neutral amino acid transporter 4 in liver development
| Project/Area Number |
20591294
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Research Institute, Osaka Medical Center for Maternal and Child Health |
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Principal Investigator |
KONDOU Hiroki Research Institute, Osaka Medical Center for Maternal and Child Health, 大学院・医学系研究科, 助教 (10373515)
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| Co-Investigator(Kenkyū-buntansha) |
MICHIGAMI Toshimi 大阪府立母子保健総合医療センター研究所, 環境影響部門, 部長 (00301804)
虫明 聡太郎 大阪大学, 医学(系)研究科(研究院), 准教授 (90291947)
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| Co-Investigator(Renkei-kenkyūsha) |
MUSHIAKE Sotaro 大阪大学, 医学系研究科, 准教授 (90291947)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 肝細胞 / アミノ酸トランスポーター / 肝発生・分化 / HNF4α / 栄養学 |
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| Research Abstract |
We screened the downstream molecules of HNF4a during liver development, sodium-coupled neutral amino acid transporter (SNAT) 4 was identified. The aim of this study is to investigate the regulation of SNAT4 by HNF4a and to clarify its roles in differentiating hepatocytes. SNAT4 mRNA showed a marked perinatal increase in mRNA levels, and was predominant among system A amino acid transporters. It was first detected in E18.5 liver in immunohistochemistry, and found in most hepatocytes after birth. Three alternative first exons were founding SNAT4 gene. Finally, over-expression experiments demonstrated that SNAT4 facilitated amino acid uptake and de novo protein synthesis in primary hepatoblasts.
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Report
(4 results)
Research Products
(6 results)
