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Development of PCR-based whole-genome analysis method and the application for the prenatal diagnosis from maternal blood

Research Project

Project/Area Number 20591308
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionShowa University

Principal Investigator

MATSUOKA Ryu  Showa University, 医学部, 講師 (20349111)

Co-Investigator(Kenkyū-buntansha) SEKIZAWA Akihiko  昭和大学, 医学部, 准教授 (10245839)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords母体血 / 有核赤血球 / 無侵襲 / 出生前診断 / ダウン症候群 / 胎児遺伝子診断 / 単一細胞 / PCR / 全遺伝子増幅 / DNA抽出 / FISH / 遺伝子診断 / real-time PCR
Research Abstract

Non-invasive prenatal diagnosis through examination of intact fetal cells circulating within maternal blood can be used to diagnose a full range of genetic disorders. Since only a limited number of fetal cells circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. Recently, we have developed the separation methods, including a lectin-based method and autoimage analyzing, leading to the improved sensitivity of genetic analysis. We recently proved the accuracy of the prenatal diagnosis of trisomy-21 and -18 by the nucleated erythrocytes analysis in maternal blood. Thus, this progress has supported the possibility of non-invasive prenatal diagnosis of genetic disorders.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (14 results)

All 2011 2010 2009 2008 Other

All Journal Article (1 results) Presentation (12 results) Remarks (1 results)

  • [Journal Article] Detection and quantification of fetal DNA in maternal plasma by using LightCycler technology.2008

    • Author(s)
      Purwosunu Y, Sekizawa A, Okai T
    • Journal Title

      Methods Mol Biol. 444

      Pages: 231-8

    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血漿を用いた無侵襲的胎児遺伝子診断(1):胎児X連鎖性疾患への応用2011

    • Author(s)
      四元淳子、関沢明彦、福田麻美、市塚清健、小出馨子、宮上哲、仲村将光、松岡隆、斎藤裕、岡井崇
    • Organizer
      第14回胎児遺伝子診断研究会学術集会
    • Place of Presentation
      金沢
    • Year and Date
      2011-02-26
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] 母体血漿を用いた無侵襲的胎児遺伝子診断(2):胎児単一遺伝子病(Achondroplasia)への応用2011

    • Author(s)
      福田麻美、四元淳子、関沢明彦、市塚清健、小出馨子、宮上哲、仲村将光、松岡隆、斎藤裕、岡井崇
    • Organizer
      第14回胎児遺伝子診断研究会学術集会
    • Place of Presentation
      金沢
    • Year and Date
      2011-02-26
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Fetal DNA Diagnosis from Maternal Blood 2.0 System2010

    • Author(s)
      Takabayashi H, Kita M, Purwosunu Y, Ikawa K, Sekizawa A, Chiba H, Kitagawa M
    • Organizer
      6th World Congress of Perinatal Medicine in developing countries.
    • Place of Presentation
      Jakarta
    • Year and Date
      2010-03-09
    • Related Report
      2010 Final Research Report
  • [Presentation] Recent Advances in Prenatal diagnosis (Special Invited Lecture)2010

    • Author(s)
      Sekizawa A
    • Organizer
      6th World Congress of Perinatal Medicine in developing countries.
    • Place of Presentation
      Jakarta
    • Year and Date
      2010-03-08
    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血中有核赤血球探索の効率化:リンパ球分離用液(Ficoll)を用いた白血球除去法の検討2010

    • Author(s)
      中村豊美、井出上公太郎、北美紀子、松本由佳、Yuditiya Purwosunu、北川道弘、関沢明彦、高村禅、高林晴夫
    • Organizer
      第13回胎児遺伝子診断研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-13
    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血における胎児遺伝子診断法:有核赤血球の濃縮と高感度FISH法の検討2010

    • Author(s)
      小木美恵子、新村卓也、眞田由親、原田俊、松本由佳、市川秀隆、中村豊美、北美紀子、Yuditiya Purwosunu、北川道弘、関沢明彦、井出上公太郎、高村禅、高林晴夫
    • Organizer
      第13回胎児遺伝子診断研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-13
    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血中有核赤血球の標本作製から回収の検討:樹脂プレートの有用性2010

    • Author(s)
      松本由佳、北美紀子、中村豊美、Yuditiya Purwosunu、北川道弘、関沢明彦、森田敏樹、末岡宗廣、高林晴夫
    • Organizer
      第13回胎児遺伝子診断研究会
    • Place of Presentation
      東京
    • Year and Date
      2010-02-13
    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血による胎児DNA診断のための有核赤血球分離法の基礎的検討2009

    • Author(s)
      北美紀子、高林晴夫、小木美恵子、関沢明彦、千葉博、北川道弘、伊川和美、松本由佳、末岡宗廣
    • Organizer
      第12回胎児遺伝子診断研究会
    • Place of Presentation
      兵庫県
    • Year and Date
      2009-02-14
    • Related Report
      2010 Final Research Report
  • [Presentation] FDD-MB (Fetal DNA Diagnosis from Maternal Blood) 2.0 System2009

    • Author(s)
      Purwosunu Y, Takabayashi H, Kita M, Ikawa K, Sekizawa A, Chiba H, Kitagawa M
    • Organizer
      6th International Conference on Circulating Nucleic Acids in Plasma and Serum (CNAPS-VI)
    • Place of Presentation
      Hong Kong
    • Related Report
      2010 Final Research Report
  • [Presentation] 母体血による胎児DNA診断(FDD-MB2.0)第一報2008

    • Author(s)
      高林晴夫、北川道弘、関沢明彦、Yuditiya Purwosunu、千葉博、伊川和美、末岡宗広
    • Organizer
      第60日本産科婦人科学会学術集会
    • Place of Presentation
      横浜
    • Year and Date
      2008-04-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Accuracy of prenatal DNA diagnosis from NRBCs in maternal blood2008

    • Author(s)
      Sekizawa A, Purwosunu Y, Chiba H, Okai T, Takabayashi H, Kita M, Kitagawa M
    • Organizer
      15th Congress of Federation of Asia-Oceania Perinatal Societies
    • Place of Presentation
      Nagoya
    • Related Report
      2010 Final Research Report
  • [Presentation] Accuracy of prenatal DNA diagnosis from NRBCs in maternal blood2008

    • Author(s)
      Sekizawa A, Purwosunu Y, Chiba H, Okai T, Takabayashi H, Kita M, Kitagawa M
    • Organizer
      14th International conference on Prenatal diagnosis and Therapy
    • Place of Presentation
      Vancouver
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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