Development of PCR-based whole-genome analysis method and the application for the prenatal diagnosis from maternal blood
| Project/Area Number |
20591308
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Showa University |
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Principal Investigator |
MATSUOKA Ryu Showa University, 医学部, 講師 (20349111)
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| Co-Investigator(Kenkyū-buntansha) |
SEKIZAWA Akihiko 昭和大学, 医学部, 准教授 (10245839)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 母体血 / 有核赤血球 / 無侵襲 / 出生前診断 / ダウン症候群 / 胎児遺伝子診断 / 単一細胞 / PCR / 全遺伝子増幅 / DNA抽出 / FISH / 遺伝子診断 / real-time PCR |
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| Research Abstract |
Non-invasive prenatal diagnosis through examination of intact fetal cells circulating within maternal blood can be used to diagnose a full range of genetic disorders. Since only a limited number of fetal cells circulate within maternal blood, procedures to enrich the cells and enable single cell analysis with high sensitivity are required. Recently, we have developed the separation methods, including a lectin-based method and autoimage analyzing, leading to the improved sensitivity of genetic analysis. We recently proved the accuracy of the prenatal diagnosis of trisomy-21 and -18 by the nucleated erythrocytes analysis in maternal blood. Thus, this progress has supported the possibility of non-invasive prenatal diagnosis of genetic disorders.
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Report
(4 results)
Research Products
(14 results)
