Novel therapeutic approach to skin diseases with protein-transduction technology

• Project/Area Number: 20591316
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Dermatology
• Research Institution: University of Yamanashi
• Principal Investigator: SHIBAGAKI Naotaka University of Yamanashi, 大学院・医学工学総合研究部, 准教授 (40262662)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: タンパク質細胞内導入 / 分子標的薬 / 癌免疫療法 / STAT3 / 阻害薬 / 癌ワクチン / STAT3阻害薬 / 皮膚腫瘍 / タンパク導入 / 分子標的療法 / 分指標的療法

Research Abstract

Protein-transduction domains (PTDs) are short stretches of cationic amino acids that enable peptides, proteins, oligonucleotides, and other reagents to efficiently enter multiple cell types. Therefore, PTDs offer unique therapeutic opportunities for the treatment of many diseases, including skin cancer. Novel STAT3 inhibitor ; R9-GRIM19 significantly reduced STAT3-dependent transcription in vitro, and tumor growth in vivo.

Research Products

• [Journal Article] Protein-transduction domainを用いた抗原性の増強 (2011)
  • Author(s): 柴垣直孝、島田眞路
  • Journal Title: 臨床免疫アレルギー科 55巻 Pages: 255-261
• [Journal Article] Novel immunotherapeutic approaches to skin cancer treatments using protein transduction technology (2011)
  • Author(s): N Shibagaki, T Okamoto, H Mitsui, T Inozume, M kanzaki, S Shimada
  • Journal Title: Journal of Dermatological Science 61 Pages: 153-161
  • NAID: 10030890246
  • Peer Reviewed
• [Journal Article] Novel Immunotherapeutic Approaches to Skin Cancer Treatments Using Protein T ransduction Technology. (Review) (2011)
  • Author(s): N Shibagaki, et al.
  • Journal Title: Journal of Dermatological Science Volume: 61(3) Pages: 153-161
  • Peer Reviewed
• [Journal Article] Protein-transduction domainを用いた抗原性の増強 (2011)
  • Author(s): 柴垣直孝, ら
  • Journal Title: 臨床免疫アレルギー科 Volume: 55(3) Pages: 255-261
• [Journal Article] A novel immunotherapeutic approach to melanoma-bearing mice hosts with protein-transduction domain containing immunogenic foreign antigens (2010)
  • Author(s): M Kanzaki, T Okamoto, H Mitsui, N Shibagaki, S Shimada
  • Journal Title: Journal of Dermatological Science 60 Pages: 84-94
  • Peer Reviewed
• [Journal Article] Overexpression of GRIM-19 in cancer cells suppresses STAT3-mediated signal transduction and cancer growth (2010)
  • Author(s): T Okamoto, T Inozume, H Mitsui, M kanzaki, K Harada, N Shibagaki, S Shimada
  • Journal Title: Molecular Cancer Therapeutics 9 Pages: 2333-2343
• [Journal Article] Intradermal injiections of polyarginin-containing immunogenic antigens preferentially elicit Tc1 and Th1 activation and antitumor immunity (2010)
  • Author(s): H Mitsui, T Okamoto, M Kanzaki, T Inozume, N Shibagaki, S Shimada
  • Journal Title: British Journal of Dermatology 162 Pages: 29-41
• [Journal Article] Overexpression of GRIM-19 in cancer cells suppresses STAT3-mediated signal transduction and cancer growth. (2010)
  • Author(s): T Okamoto, et al.
  • Journal Title: Molecular Cancer Therapeutics Volume: 9 Pages: 2333-2343
  • Peer Reviewed
• [Journal Article] A Novel Immunotherapeutic Approach to Melanoma-bearing Hosts with Protein-transduction Domain-containing Immunogenic Foreign Antigens. (2010)
  • Author(s): M Kanzaki, et al.
  • Journal Title: Journal of Dermatological Science Volume: 60 Pages: 84-94
  • Peer Reviewed
• [Journal Article] Intradermal injections of polyarginine-containing immunogenic antigens preferentially elicit Tcl and Thl activation and antitumor immunity (2010)
  • Author(s): H Mitsui, et al.
  • Journal Title: British Journal of Dermatology Volume: 162 Pages: 29-41
  • Peer Reviewed
• [Journal Article] Intradermal injections of polyarginine-containing immunogenic Ags preferentially elicit Tcl and Th1 activation and antitumor immunity (2010)
  • Author(s): H.Mitsui
  • Journal Title: British Journal of Dermatology 162 Pages: 29-41
  • Peer Reviewed
• [Journal Article] 抗原ペプチドの樹状細胞への遺伝子導入法と免疫原性 (2009)
  • Author(s): 柴垣直孝
  • Journal Title: 臨床免疫アレルギー科 51 Pages: 586-594
• [Journal Article] 抗原ペプチドの樹状細胞への遺伝子導入法と免疫原性 (2009)
  • Author(s): 柴垣直孝
  • Journal Title: 臨床免疫 アレルギー科 51 Pages: 586-594
• [Journal Article] Intradermal Injection of Polyarginine-containing Immunogenic Antigens Preferentially Elicit Tc1 and Th1 Activation and Antitumor Immunity
  • Author(s): Mitsui H., Okamoto T., Kanzaki M., Inozume T., Shibagaki N., Shimada S.
  • Journal Title: British Journal of Dermatology (In press)
  • Peer Reviewed
• [Presentation] 癌免疫療法のトピックスと展望 (2011)
  • Author(s): 柴垣直孝
  • Organizer: 第74回日本皮膚科学会東京支部学会
  • Place of Presentation: 東京
  • Year and Date: 2011-02-12
• [Presentation] 癌免疫療法のトピックスと展望 (2011)
  • Author(s): 柴垣直孝
  • Organizer: 第74回日本皮膚科学会東京支部学術大会
  • Place of Presentation: 東京(お台場)
  • Year and Date: 2011-02-12
• [Presentation] Intratumoral injections of a novel STAT3 inhibitor (R9-GRIM19)enhance antitumor effects combined with cancer immunotherapy in melanoma-bearing mice (2010)
  • Author(s): 岡本崇、柴垣直孝、島田眞路
  • Organizer: 第35回に本研究皮膚科学会
  • Place of Presentation: 和歌山
  • Year and Date: 2010-12-04
• [Presentation] Intratumoral injections of a novel STAT3 inhibitor (R9-GRIM19) enhance antitumor effects combined with cancer immunotherapy (2010)
  • Author(s): 岡本崇
  • Organizer: 第35回日本研究皮膚科学会学術大会
  • Place of Presentation: 和歌山
  • Year and Date: 2010-12-04
• [Presentation] Intratumoral injections of a novel STAT3 inhibitor (R9-GRIM19) elicited strong antitumor effects by provoking innate and acquired immunity (2010)
  • Author(s): T Okamoto, et al.
  • Organizer: 70th Society for Investigative Dermatology
  • Place of Presentation: Atlanta GA. USA
  • Year and Date: 2010-05-07
• [Presentation] 腫瘍免疫療法の新たな展望 (2010)
  • Author(s): 柴垣直孝
  • Organizer: 第109回日本皮膚科学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-04-17
• [Presentation] 腫瘍免疫療法の新たな展望(教育講演) (2010)
  • Author(s): 柴垣直孝
  • Organizer: 第109回日本皮膚科学会総会学術大会
  • Place of Presentation: 大阪(中之島)
  • Year and Date: 2010-04-17
• [Presentation] Overexpression of GRIM19 in cancer cells suppresses STAT3-mediated signal transduction and cancer growth (2009)
  • Author(s): 柴垣直孝
  • Organizer: World Congress of Inflammation 2009
  • Place of Presentation: 東京
  • Year and Date: 2009-07-08
• [Presentation] Overexpression of GRIM19 in cancer cells suppresses STAT3-mediated signal transduction and cancer growth (2009)
  • Author(s): Naotaka Shibagaki
  • Organizer: World Congress of Inflammation 2009
  • Place of Presentation: 東京都新宿
  • Year and Date: 2009-07-08
• [Presentation] Over expression of GRIM19 in Cancer Cells Suppressed STAT3-mediated Signal Transduct (2009)
  • Author(s): Okamoto T., et al.
  • Organizer: International Society for Investigative Dermatology
  • Place of Presentation: Kyoto
  • Year and Date: 2009-05-17
• [Presentation] A novel immunotherapeutic approach to cancer-bearing host with protein-transduction domain-containing immunogenic foreign antigens (2008)
  • Author(s): 柴垣直孝
  • Organizer: International Investigative Dermatology 2008
  • Place of Presentation: 京都
  • Year and Date: 2008-05-17
• [Remarks] ホームページ等
• [Remarks]
  • URL: http://erdb.yamanashi.ac.jp/rdb/A_DispInfo.Scholar/0/7FB51F55041ACE1A.html